Restriction map for
pFN21AE1562
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI MamI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 903 CGCG CG^CG
AcsI 1306 AAATTT [GA]^AATT[CT]
AocI 58 CCTCAGG CC^T[ACGT]AGG
ApaI 510 GGGCCC GGGCC^C
ApoI 1306 AAATTT [GA]^AATT[CT]
AspHI 961 GAGCTC G[AT]GC[AT]^C
BanI 298 GGCACC G^G[CT][GA]CC
BbsI 1378 GAAGAC GAAGAC(2/6)
BbvCI 922 CCTCAGC CCTCAGC(-5/-2)
BcnI 879 CCGGG CC^[GC]GG
BlpI 958 GCTGAGC GC^T[ACGT]AGC
BpmI 862 CTCCAG CTGGAG(16/14)
Bpu1102I 958 GCTGAGC GC^T[ACGT]AGC
BpuAI 1378 GAAGAC GAAGAC(2/6)
BsgI 240 CTGCAC GTGCAG(16/14)
BsiHKAI 961 GAGCTC G[AT]GC[AT]^C
BsrFI 602 GCCGGC [GA]^CCGG[CT]
BsrI 1194 CCAGT ACTGG(1/-1)
BstAPI 1140 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 903 CGCG CG^CG
BstXI 455 CCAATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 58 CCTCAGG CC^T[ACGT]AGG
CelII 958 GCTGAGC GC^T[ACGT]AGC
CfoI 896 GCGC GCG^C
Cfr10I 602 GCCGGC [GA]^CCGG[CT]
DsaI 902 CCGCGG C^C[GA][CT]GG
Eco81I 58 CCTCAGG CC^T[ACGT]AGG
EcoNI 406 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 896 GCGC GCG^C
HinP1I 896 GCGC G^CGC
HincII 352 GTCAAC GT[CT]^[GA]AC
HindII 352 GTCAAC GT[CT]^[GA]AC
KspI 902 CCGCGG CCGC^GG
MvnI 903 CGCG CG^CG
NaeI 602 GCCGGC GCC^GGC
NciI 879 CCGGG CC^[GC]GG
NgoMIV 602 GCCGGC G^CCGGC
NheI 429 GCTAGC G^CTAGC
PflMI 385 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 234 CAGCTG CAG^CTG
SacI 961 GAGCTC GAGCT^C
SacII 902 CCGCGG CCGC^GG
ScaI 1154 AGTACT AGT^ACT
StuI 1095 AGGCCT AGG^CCT
Van91I 385 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1218 CCACTGCCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 546 GTAC GT^AC
1155 GTAC
AlwI 63 GGATC GGATC(4/5)
680 GGATC
AvaI 766 CTCGAG C^[CT]CG[GA]G
909 CTCGAG
AvaII 416 GGACC G^G[AT]CC
1014 GGACC
BanII 510 GGGCCC G[GA]GC[CT]^C
961 GAGCTC
BmyI 510 GGGCCC G[AGT]GC[ACT]^C
961 GAGCTC
BsmBI 8 CGTCTC CGTCTC(1/5)
1435 GAGACG
BsoBI 766 CTCGAG C^[CT]CG[GA]G
909 CTCGAG
Bsp1286I 510 GGGCCC G[AGT]GC[ACT]^C
961 GAGCTC
BsrBI 204 CCGCTC CCGCTC(-3/-3)
1239 CCGCTC
BsrDI 288 CATTGC GCAATG(2/0)
995 GCAATG
BstYI 63 GGATCT [GA]^GATC[CT]
680 GGATCT
EarI 777 GAAGAG CTCTTC(1/4)
1162 GAAGAG
FauI 203 CCCGC CCCGC(4/6)
1238 CCCGC
HapII 603 CCGG C^CGG
879 CCGG
HpaII 603 CCGG C^CGG
879 CCGG
Ksp632I 777 GAAGAG CTCTTC(1/4)
1162 GAAGAG
MflI 63 GGATCT [GA]^GATC[CT]
680 GGATCT
MspI 603 CCGG C^CGG
879 CCGG
PaeR7I 766 CTCGAG C^TCGAG
909 CTCGAG
PpuMI 415 GGGACCC [GA]G^G[AT]CC[CT]
1013 AGGACCT
PstI 528 CTGCAG CTGCA^G
613 CTGCAG
RsaI 546 GTAC GT^AC
1155 GTAC
TfiI 168 GAATC G^A[AT]TC
1406 GAATC
XhoI 766 CTCGAG C^TCGAG
909 CTCGAG
XhoII 63 GGATCT [GA]^GATC[CT]
680 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwNI BbvI BfaI
BsaJI BsiYI BslI BsmAI BsmFI
BspMI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I