Restriction map for
pFN21AE1494
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AlwI 123 GGATC GGATC(4/5)
AocI 378 CCTCAGG CC^T[ACGT]AGG
ApaI 224 GGGCCC GGGCC^C
BanII 224 GGGCCC G[GA]GC[CT]^C
BcgI 501 GCAGGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 389 CTAG C^TAG
BglI 375 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 224 GGGCCC G[AGT]GC[ACT]^C
Bsp1286I 224 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 708 TGTACA T^GTACA
BsrFI 433 GCCGGC [GA]^CCGG[CT]
BsrGI 708 TGTACA T^GTACA
BsrI 713 ACTGG ACTGG(1/-1)
BssSI 680 CACGAG CACGAG(-5/-1)
Bsu36I 378 CCTCAGG CC^T[ACGT]AGG
Cfr10I 433 GCCGGC [GA]^CCGG[CT]
EaeI 431 TGGCCG [CT]^GGCC[GA]
EarI 138 GAAGAG CTCTTC(1/4)
Eco81I 378 CCTCAGG CC^T[ACGT]AGG
EcoT14I 218 CCTTGG C^C[AT][AT]GG
FauI 92 CCCGC CCCGC(4/6)
HapII 434 CCGG C^CGG
HindIII 702 AAGCTT A^AGCTT
HpaII 434 CCGG C^CGG
Ksp632I 138 GAAGAG CTCTTC(1/4)
MaeI 389 CTAG C^TAG
MspI 434 CCGG C^CGG
NaeI 433 GCCGGC GCC^GGC
NgoMIV 433 GCCGGC G^CCGGC
PleI 604 GACTC GAGTC(4/5)
PpuMI 569 GGGACCC [GA]G^G[AT]CC[CT]
PstI 334 CTGCAG CTGCA^G
SfaNI 49 GATGC GCATC(5/9)
SfcI 334 CTGCAG C^T[GA][CT]AG
SmlI 699 CTCAAG C^T[CT][GA]AG
SphI 671 GCATGC GCATG^C
SspBI 708 TGTACA T^GTACA
StyI 218 CCTTGG C^C[AT][AT]GG
TaqI 510 TCGA T^CGA
TfiI 520 GAATC G^A[AT]TC
Tsp45I 214 GTCAC ^GT[GC]AC
TthHB8I 510 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 548 GTAC GT^AC
709 GTAC
AvaII 359 GGACC G^G[AT]CC
570 GGACC
BpmI 340 CTGGAG CTGGAG(16/14)
608 CTGGAG
BstF5I 282 CATCC GGATG(2/0)
289 CATCC
DdeI 305 CTAAG C^T[ACGT]AG
379 CTCAG
FokI 282 CATCC GGATG(9/13)
289 CATCC
HinfI 520 GAATC G^A[ACGT]TC
604 GACTC
MaeIII 214 GTCAC ^GT[ACGT]AC
626 GTAAC
MseI 581 TTAA T^TAA
596 TTAA
MspA1I 182 CAGCTG C[AC]G^C[GT]G
337 CAGCTG
NspI 421 ACATGC [GA]CATG^[CT]
671 GCATGC
PvuII 182 CAGCTG CAG^CTG
337 CAGCTG
RsaI 548 GTAC GT^AC
709 GTAC
Tru9I 581 TTAA T^TAA
596 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BssKI
BstNI Cac8I Cfr13I DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HphI
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI TspRI