Restriction map for
pFN21AE6726
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccIII AclI AcyI AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BamHI BbeI BbrPI
BciVI BclI BglI BlnI BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1166 GGTACC G^GTACC
AccI 820 GTCGAC GT^[AC][GT]AC
AflII 814 CTTAAG C^TTAAG
AocI 438 CCTGAGG CC^T[ACGT]AGG
Asp718I 1166 GGTACC G^GTACC
AspI 388 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 842 CCCGAG C^[CT]CG[GA]G
BalI 1753 TGGCCA TGG^CCA
BanI 1166 GGTACC G^G[CT][GA]CC
BbvCI 925 GCTGAGG CCTCAGC(-5/-2)
BcgI 822 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 235 CTAG C^TAG
BfrI 814 CTTAAG C^TTAAG
BsaI 25 GAGACC GGTCTC(1/5)
BsaWI 1748 TCCGGT [AT]^CCGG[AT]
BsgI 858 GTGCAG GTGCAG(16/14)
BsmBI 1723 CGTCTC CGTCTC(1/5)
BsoBI 842 CCCGAG C^[CT]CG[GA]G
BspHI 1898 TCATGA T^CATGA
BsrBI 700 GAGCGG CCGCTC(-3/-3)
BstAPI 271 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1654 GGTTACC G^GT[ACGT]ACC
BstPI 1654 GGTTACC G^GT[ACGT]ACC
Bsu36I 438 CCTGAGG CC^T[ACGT]AGG
DrdI 2047 GACATTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1753 TGGCCA [CT]^GGCC[GA]
Eco81I 438 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1654 GGTTACC G^GT[ACGT]ACC
EcoRI 547 GAATTC G^AATTC
KpnI 1166 GGTACC GGTAC^C
MaeI 235 CTAG C^TAG
MluNI 1753 TGGCCA TGG^CCA
MscI 1753 TGGCCA TGG^CCA
NspI 1706 GCATGT [GA]CATG^[CT]
PflFI 388 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 777 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 52 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1898 TCATGA T^CATGA
SalI 820 GTCGAC G^TCGAC
SapI 576 GAAGAGC GCTCTTC(1/4)
SexAI 1910 ACCAGGT A^CC[AT]GGT
TaqI 821 TCGA T^CGA
Tth111I 388 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 821 TCGA T^CGA
Van91I 777 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 547 GAATTC [GA]^AATT[CT]
1447 AAATTT
ApaI 737 GGGCCC GGGCC^C
1104 GGGCCC
ApoI 547 GAATTC [GA]^AATT[CT]
1447 AAATTT
BcnI 882 CCGGG CC^[GC]GG
1520 CCGGG
BglII 1328 AGATCT A^GATCT
1820 AGATCT
BsmI 1890 GAATGC GAATGC(1/-1)
1495 GCATTC
BsrDI 1305 CATTGC GCAATG(2/0)
1503 CATTGC
EcoNI 810 CCTGCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1263 CCTCCAGAAGG
HgaI 268 GACGC GACGC(5/10)
392 GCGTC
HincII 820 GTCGAC GT[CT]^[GA]AC
1203 GTTGAC
HindII 820 GTCGAC GT[CT]^[GA]AC
1203 GTTGAC
MaeII 164 ACGT A^CGT
220 ACGT
NciI 882 CCGGG CC^[GC]GG
1520 CCGGG
NdeI 175 CATATG CA^TATG
1757 CATATG
PleI 331 GAGTC GAGTC(4/5)
918 GAGTC
PpuMI 956 GGGACCT [GA]G^G[AT]CC[CT]
1550 GGGACCT
SmlI 814 CTTAAG C^T[CT][GA]AG
2011 CTCAAG
XcmI 584 CCAGAGAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1502 CCATTGCAGTTGTGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbsI
BbvI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII