Restriction map for
pFN21AB8537
##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BciVI
BclI BfrI BlnI BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I ClaI CpoI DraIII DrdI
Eam1105I EcoRI EcoRV FbaI FseI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NspV
PacI PaeR7I PmeI PshBI PvuI
RcaI RsrII SacI SacII SapI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 2605 GTCGAC GT^[AC][GT]AC
AclI 408 AACGTT AA^CGTT
AgeI 698 ACCGGT A^CCGGT
Aor51HI 2674 AGCGCT AGC^GCT
AvaI 2333 CCCGGG C^[CT]CG[GA]G
AviII 2780 TGCGCA TGC^GCA
BamHI 731 GGATCC G^GATCC
BbrPI 769 CACGTG CAC^GTG
BbvCI 2061 GCTGAGG CCTCAGC(-5/-2)
BcgI 15 GCACAGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 769 CACGTG [CT]AC^GT[GA]
BsiEI 77 CGGCCG CG[GA][CT]^CG
BsmI 232 GCATTC GAATGC(1/-1)
BsoBI 2333 CCCGGG C^[CT]CG[GA]G
BsrFI 698 ACCGGT [GA]^CCGG[CT]
BssHII 1964 GCGCGC G^CGCGC
BstAPI 1467 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 698 ACCGGT [GA]^CCGG[CT]
DraI 958 TTTAAA TTT^AAA
EagI 77 CGGCCG C^GGCCG
EclXI 77 CGGCCG C^GGCCG
Eco47III 2674 AGCGCT AGC^GCT
Eco52I 77 CGGCCG C^GGCCG
EcoT22I 2837 ATGCAT ATGCA^T
FspI 2780 TGCGCA TGC^GCA
HincII 2605 GTCGAC GT[CT]^[GA]AC
HindII 2605 GTCGAC GT[CT]^[GA]AC
NruI 2277 TCGCGA TCG^CGA
NsiI 2837 ATGCAT ATGCA^T
PflMI 2763 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 698 ACCGGT A^CCGGT
PmaCI 769 CACGTG CAC^GTG
PmlI 769 CACGTG CAC^GTG
PpuMI 2297 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 336 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 408 AACGTT AA^CGTT
SalI 2605 GTCGAC G^TCGAC
SbfI 1536 CCTGCAGG CCTGCA^GG
SfiI 1926 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2333 CCCGGG CCC^GGG
Sse8387I 1536 CCTGCAGG CCTGCA^GG
TfiI 620 GAATC G^A[AT]TC
Van91I 2763 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2333 CCCGGG C^CCGGG
XmaI 2333 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1965 CGCG CG^CG
2278 CGCG
AocI 226 CCTCAGG CC^T[ACGT]AGG
2509 CCTGAGG
Asp700I 994 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2859 GAAGGCTTTC
BalI 1385 TGGCCA TGG^CCA
1907 TGGCCA
BbeI 872 GGCGCC GGCGC^C
1083 GGCGCC
BlpI 146 GCTTAGC GC^T[ACGT]AGC
2139 GCTGAGC
Bpu1102I 146 GCTTAGC GC^T[ACGT]AGC
2139 GCTGAGC
BsaWI 698 ACCGGT [AT]^CCGG[AT]
2654 TCCGGT
BsrBI 1273 GAGCGG CCGCTC(-3/-3)
1639 GAGCGG
BssSI 1552 CACGAG CACGAG(-5/-1)
2731 CACGAG
BstEII 718 GGTAACC G^GT[ACGT]ACC
1705 GGTAACC
BstPI 718 GGTAACC G^GT[ACGT]ACC
1705 GGTAACC
BstUI 1965 CGCG CG^CG
2278 CGCG
BstXI 65 CCATCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1852 CCAGGTGAATGG
Bsu36I 226 CCTCAGG CC^T[ACGT]AGG
2509 CCTGAGG
CelII 146 GCTTAGC GC^T[ACGT]AGC
2139 GCTGAGC
Eco81I 226 CCTCAGG CC^T[ACGT]AGG
2509 CCTGAGG
EcoNI 795 CCTACTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2884 CCTCCCGAAGG
EcoO65I 718 GGTAACC G^GT[ACGT]ACC
1705 GGTAACC
HgaI 2025 GACGC GACGC(5/10)
2323 GACGC
KasI 872 GGCGCC G^GCGCC
1083 GGCGCC
MluNI 1385 TGGCCA TGG^CCA
1907 TGGCCA
MscI 1385 TGGCCA TGG^CCA
1907 TGGCCA
MvnI 1965 CGCG CG^CG
2278 CGCG
NarI 872 GGCGCC GG^CGCC
1083 GGCGCC
PleI 316 GACTC GAGTC(4/5)
1843 GAGTC
SfoI 872 GGCGCC GGC^GCC
1083 GGCGCC
SmlI 1315 CTTGAG C^T[CT][GA]AG
2199 CTTGAG
StuI 1439 AGGCCT AGG^CCT
2881 AGGCCT
XmnI 994 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2859 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AspI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII