Restriction map for
pFN21AE6353
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbvCI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI MamI MfeI MluI
MroI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI ScaI SexAI
SfiI SgrAI SmlI SnaBI SpeI
SspBI SspI StuI SwaI Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 69 enzymes) #
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Acc65I 883 GGTACC G^GTACC
AccII 61 CGCG CG^CG
AcyI 67 GGCGCC G[GA]^CG[CT]C
Alw44I 709 GTGCAC G^TGCAC
AocI 124 CCTGAGG CC^T[ACGT]AGG
ApaLI 709 GTGCAC G^TGCAC
Asp718I 883 GGTACC G^GTACC
AspHI 709 GTGCAC G[AT]GC[AT]^C
AvaI 53 CCCGGG C^[CT]CG[GA]G
BamHI 835 GGATCC G^GATCC
BbeI 67 GGCGCC GGCGC^C
BbrPI 712 CACGTG CAC^GTG
BbsI 253 GTCTTC GAAGAC(2/6)
BcgI 525 CGAGGGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 918 GGATAC GTATCC(6/5)
BfaI 626 CTAG C^TAG
BglI 1039 GCCGTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 253 GTCTTC GAAGAC(2/6)
BsaAI 712 CACGTG [CT]AC^GT[GA]
BsaHI 67 GGCGCC G[GA]^CG[CT]C
BsiHKAI 709 GTGCAC G[AT]GC[AT]^C
BsmBI 857 GAGACG CGTCTC(1/5)
BsoBI 53 CCCGGG C^[CT]CG[GA]G
BspHI 305 TCATGA T^CATGA
BspMI 1101 GCAGGT ACCTGC(4/8)
BsrBI 842 CCGCTC CCGCTC(-3/-3)
BsrDI 893 GCAATG GCAATG(2/0)
BstUI 61 CGCG CG^CG
BstYI 835 GGATCC [GA]^GATC[CT]
Bsu36I 124 CCTGAGG CC^T[ACGT]AGG
DpnII 836 GATC ^GATC
Eco81I 124 CCTGAGG CC^T[ACGT]AGG
EcoNI 120 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 831 CCTTGG C^C[AT][AT]GG
HaeII 67 GGCGCC [GA]GCGC^[CT]
HgaI 692 GCGTC GACGC(5/10)
Hin1I 67 GGCGCC G[GA]^CG[CT]C
HphI 45 GGTGA GGTGA(8/7)
KasI 67 GGCGCC G^GCGCC
KpnI 883 GGTACC GGTAC^C
KspI 60 CCGCGG CCGC^GG
MaeI 626 CTAG C^TAG
MaeIII 466 GTCAC ^GT[ACGT]AC
MboI 836 GATC ^GATC
MflI 835 GGATCC [GA]^GATC[CT]
MseI 577 TTAA T^TAA
MvnI 61 CGCG CG^CG
NarI 67 GGCGCC GG^CGCC
NdeII 836 GATC ^GATC
NspI 210 GCATGC [GA]CATG^[CT]
PmaCI 712 CACGTG CAC^GTG
PmlI 712 CACGTG CAC^GTG
PvuII 1089 CAGCTG CAG^CTG
RcaI 305 TCATGA T^CATGA
SacII 60 CCGCGG CCGC^GG
SapI 664 GAAGAGC GCTCTTC(1/4)
Sau3AI 836 GATC ^GATC
SbfI 1098 CCTGCAGG CCTGCA^GG
SfoI 67 GGCGCC GGC^GCC
SmaI 53 CCCGGG CCC^GGG
SphI 210 GCATGC GCATG^C
Sse8387I 1098 CCTGCAGG CCTGCA^GG
StyI 831 CCTTGG C^C[AT][AT]GG
Tru9I 577 TTAA T^TAA
Tsp45I 466 GTCAC ^GT[GC]AC
XcmI 569 CCAACCTGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 835 GGATCC [GA]^GATC[CT]
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwI 836 GATCC GGATC(4/5)
835 GGATC
AlwNI 14 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1132 CAGTGCCTG
ApaI 604 GGGCCC GGGCC^C
971 GGGCCC
BalI 299 TGGCCA TGG^CCA
1010 TGGCCA
BsaI 31 GAGACC GGTCTC(1/5)
450 GGTCTC
BseRI 127 GAGGAG GAGGAG(10/8)
652 GAGGAG
BstAPI 559 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
634 GCAGAGTATGC
BstBI 866 TTCGAA TT^CGAA
904 TTCGAA
BstXI 35 CCACTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
134 CCAAACCTGTGG
CfoI 68 GCGC GCG^C
1140 GCGC
DsaI 60 CCGCGG C^C[GA][CT]GG
618 CCACGG
EaeI 299 TGGCCA [CT]^GGCC[GA]
1010 TGGCCA
HhaI 68 GCGC GCG^C
1140 GCGC
HinP1I 68 GCGC G^CGC
1140 GCGC
MaeII 713 ACGT A^CGT
860 ACGT
MluNI 299 TGGCCA TGG^CCA
1010 TGGCCA
MscI 299 TGGCCA TGG^CCA
1010 TGGCCA
NspV 866 TTCGAA TT^CGAA
904 TTCGAA
PflMI 13 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
321 CCACTTGCTGG
PpuMI 170 GGGACCC [GA]G^G[AT]CC[CT]
722 GGGACCT
PstI 730 CTGCAG CTGCA^G
1099 CTGCAG
SfcI 730 CTGCAG C^T[GA][CT]AG
1099 CTGCAG
SfuI 866 TTCGAA TT^CGAA
904 TTCGAA
TfiI 869 GAATC G^A[AT]TC
1082 GATTC
Van91I 13 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
321 CCACTTGCTGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I