Restriction map for
pFN21AE3673
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BalI BamHI
BanII BbrPI BbsI BbvCI BciVI
BclI BglI BpmI BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I Cfr10I
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT22I FauI FbaI FseI FspI
HgaI HindIII HpaI KpnI KspI
MamI MluI MluNI MroI MscI
MslI MspA1I MvaI MvnI MwoI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI Tsp45I Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 49 GGCGCC G[GA]^CG[CT]C
AflII 645 CTTAAG C^TTAAG
AlwI 795 GGATC GGATC(4/5)
AspHI 106 GAGCAC G[AT]GC[AT]^C
AvaI 1103 CTCGGG C^[CT]CG[GA]G
AvrII 220 CCTAGG C^CTAGG
BanI 49 GGCGCC G^G[CT][GA]CC
BbeI 49 GGCGCC GGCGC^C
BcgI 900 CGACCTTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 828 CCGGG CC^[GC]GG
BfrI 645 CTTAAG C^TTAAG
BglII 255 AGATCT A^GATCT
BlnI 220 CCTAGG C^CTAGG
BlpI 248 GCTTAGC GC^T[ACGT]AGC
BmyI 106 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 248 GCTTAGC GC^T[ACGT]AGC
BsaHI 49 GGCGCC G[GA]^CG[CT]C
BsaWI 96 TCCGGT [AT]^CCGG[AT]
BsgI 675 GTGCAG GTGCAG(16/14)
BsiHKAI 106 GAGCAC G[AT]GC[AT]^C
BsoBI 1103 CTCGGG C^[CT]CG[GA]G
Bsp1286I 106 GAGCAC G[AGT]GC[ACT]^C
BspDI 806 ATCGAT AT^CGAT
BspHI 972 TCATGA T^CATGA
BspMI 139 ACCTGC ACCTGC(4/8)
BssKI 828 CCGGG ^CC[ACGT]GG
BstYI 255 AGATCT [GA]^GATC[CT]
CelII 248 GCTTAGC GC^T[ACGT]AGC
CfoI 50 GCGC GCG^C
ClaI 806 ATCGAT AT^CGAT
DraI 784 TTTAAA TTT^AAA
DsaI 44 CCATGG C^C[GA][CT]GG
EcoRV 1057 GATATC GAT^ATC
HaeII 49 GGCGCC [GA]GCGC^[CT]
HaeIII 946 GGCC GG^CC
HhaI 50 GCGC GCG^C
Hin1I 49 GGCGCC G[GA]^CG[CT]C
HinP1I 50 GCGC G^CGC
HphI 871 GGTGA GGTGA(8/7)
KasI 49 GGCGCC G^GCGCC
MaeIII 1082 GTTAC ^GT[ACGT]AC
MfeI 404 CAATTG C^AATTG
MflI 255 AGATCT [GA]^GATC[CT]
MunI 404 CAATTG C^AATTG
NarI 49 GGCGCC GG^CGCC
NciI 828 CCGGG CC^[GC]GG
NcoI 44 CCATGG C^CATGG
RcaI 972 TCATGA T^CATGA
ScrFI 828 CCGGG CC^[ACGT]GG
SfoI 49 GGCGCC GGC^GCC
SmlI 645 CTTAAG C^T[CT][GA]AG
SspI 469 AATATT AAT^ATT
SwaI 783 ATTTAAAT ATTT^AAAT
TfiI 759 GATTC G^A[AT]TC
XhoII 255 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AfaI 41 GTAC GT^AC
897 GTAC
Asp700I 88 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
193 GAAAAAGTTC
AvaII 82 GGTCC G^G[AT]CC
934 GGTCC
BbvI 578 GCAGC GCAGC(8/12)
710 GCAGC
BfaI 221 CTAG C^TAG
763 CTAG
BsaJI 44 CCATGG C^C[ACGT][ACGT]GG
220 CCTAGG
BsiYI 504 CCCAACGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
937 CCCAATCGAGG
BslI 504 CCCAACGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
937 CCCAATCGAGG
BsmAI 19 GTCTC GTCTC(1/5)
1101 GTCTC
BsmFI 1106 GGGAC GGGAC(10/14)
935 GTCCC
BstF5I 1086 CATCC GGATG(2/0)
734 GGATG
Cfr13I 82 GGTCC G^G[ACGT]CC
934 GGTCC
DpnII 256 GATC ^GATC
796 GATC
EcoRI 379 GAATTC G^AATTC
1036 GAATTC
EcoT14I 44 CCATGG C^C[AT][AT]GG
220 CCTAGG
Fnu4HI 578 GCAGC GC^[ACGT]GC
710 GCAGC
FokI 1086 CATCC GGATG(9/13)
734 GGATG
HapII 97 CCGG C^CGG
828 CCGG
HpaII 97 CCGG C^CGG
828 CCGG
ItaI 578 GCAGC GC^[ACGT]GC
710 GCAGC
MaeI 221 CTAG C^TAG
763 CTAG
MaeII 508 ACGT A^CGT
614 ACGT
MboI 256 GATC ^GATC
796 GATC
MnlI 32 CCTC CCTC(7/6)
944 GAGG
MspI 97 CCGG C^CGG
828 CCGG
NdeII 256 GATC ^GATC
796 GATC
NlaIV 49 GGCGCC GG[ACGT]^[ACGT]CC
934 GGTCCC
NspI 244 ACATGC [GA]CATG^[CT]
359 ACATGC
RsaI 41 GTAC GT^AC
897 GTAC
Sau3AI 256 GATC ^GATC
796 GATC
Sau96I 82 GGTCC G^G[ACGT]CC
934 GGTCC
StyI 44 CCATGG C^C[AT][AT]GG
220 CCTAGG
TaqI 807 TCGA T^CGA
942 TCGA
TseI 578 GCAGC G^C[AT]GC
710 GCAGC
TthHB8I 807 TCGA T^CGA
942 TCGA
XcmI 35 CCATCTGTACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
499 CCAGACCCAACGTGG
XmnI 88 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
193 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AcsI AluI ApoI DdeI EarI
HincII HindII HinfI Ksp632I MboII
MseI NlaIII PleI SfaNI Tru9I
Tsp509I TspRI