Restriction map for
pFN21AE4540
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BfrI BlnI BsaHI BsiWI
BsmBI BsmI Bsp1407I BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I Eco47III EcoO109I EcoO65I EcoRV
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MunI NarI NcoI NdeI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1307 GTAGAC GT^[AC][GT]AC
AccII 521 CGCG CG^CG
BanI 590 GGTGCC G^G[CT][GA]CC
BclI 120 TGATCA T^GATCA
BglI 1390 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1529 AGATCT A^GATCT
BsaAI 493 TACGTG [CT]AC^GT[GA]
BsaBI 465 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1084 GAGACC GGTCTC(1/5)
BsgI 1218 CTGCAC GTGCAG(16/14)
BspDI 1630 ATCGAT AT^CGAT
BspHI 515 TCATGA T^CATGA
BsrDI 933 CATTGC GCAATG(2/0)
BsrFI 755 GCCGGC [GA]^CCGG[CT]
BstUI 521 CGCG CG^CG
CfoI 857 GCGC GCG^C
Cfr10I 755 GCCGGC [GA]^CCGG[CT]
ClaI 1630 ATCGAT AT^CGAT
EagI 842 CGGCCG C^GGCCG
EarI 184 GAAGAG CTCTTC(1/4)
EclXI 842 CGGCCG C^GGCCG
Eco52I 842 CGGCCG C^GGCCG
EcoNI 66 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1747 GAATTC G^AATTC
EcoT22I 1426 ATGCAT ATGCA^T
FbaI 120 TGATCA T^GATCA
HaeII 856 AGCGCC [GA]GCGC^[CT]
HhaI 857 GCGC GCG^C
HinP1I 857 GCGC G^CGC
Ksp632I 184 GAAGAG CTCTTC(1/4)
MamI 465 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 967 TTAA T^TAA
MvnI 521 CGCG CG^CG
NaeI 755 GCCGGC GCC^GGC
NgoMIV 755 GCCGGC G^CCGGC
NheI 1128 GCTAGC G^CTAGC
NotI 841 GCGGCCGC GC^GGCCGC
NsiI 1426 ATGCAT ATGCA^T
PstI 1400 CTGCAG CTGCA^G
RcaI 515 TCATGA T^CATGA
SacI 1173 GAGCTC GAGCT^C
SfcI 1400 CTGCAG C^T[GA][CT]AG
SmaI 901 CCCGGG CCC^GGG
SphI 506 GCATGC GCATG^C
TfiI 484 GATTC G^A[AT]TC
Tru9I 967 TTAA T^TAA
XmaCI 901 CCCGGG C^CCGGG
XmaI 901 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1266 TCCGGA T^CCGGA
1550 TCCGGA
AcsI 1570 AAATTC [GA]^AATT[CT]
1747 GAATTC
AlwNI 855 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1394 CAGCGGCTG
AocI 195 CCTCAGG CC^T[ACGT]AGG
817 CCTGAGG
ApoI 1570 AAATTC [GA]^AATT[CT]
1747 GAATTC
Asp700I 369 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1270 GAAGGTGTTC
AspHI 787 GAGCAC G[AT]GC[AT]^C
1173 GAGCTC
BanII 447 GGGCTC G[GA]GC[CT]^C
1173 GAGCTC
BbvCI 7 CCTCAGC CCTCAGC(-5/-2)
327 CCTCAGC
BfaI 1129 CTAG C^TAG
1327 CTAG
BlpI 103 GCTGAGC GC^T[ACGT]AGC
1491 GCTTAGC
Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC
1491 GCTTAGC
BseAI 1266 TCCGGA T^CCGGA
1550 TCCGGA
BsiEI 339 CGGTCG CG[GA][CT]^CG
842 CGGCCG
BsiHKAI 787 GAGCAC G[AT]GC[AT]^C
1173 GAGCTC
BsmFI 1012 GGGAC GGGAC(10/14)
1321 GTCCC
BspEI 1266 TCCGGA T^CCGGA
1550 TCCGGA
BspMI 662 ACCTGC ACCTGC(4/8)
1311 ACCTGC
BstYI 1529 AGATCT [GA]^GATC[CT]
1832 AGATCC
Bsu36I 195 CCTCAGG CC^T[ACGT]AGG
817 CCTGAGG
CelII 103 GCTGAGC GC^T[ACGT]AGC
1491 GCTTAGC
Eco81I 195 CCTCAGG CC^T[ACGT]AGG
817 CCTGAGG
FauI 707 GCGGG CCCGC(4/6)
863 GCGGG
HincII 619 GTCAAC GT[CT]^[GA]AC
1540 GTCAAC
HindII 619 GTCAAC GT[CT]^[GA]AC
1540 GTCAAC
MaeI 1129 CTAG C^TAG
1327 CTAG
MflI 1529 AGATCT [GA]^GATC[CT]
1832 AGATCC
MroI 1266 TCCGGA T^CCGGA
1550 TCCGGA
MslI 130 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1713 CATCAACATG
NspI 506 GCATGC [GA]CATG^[CT]
791 ACATGC
PaeR7I 91 CTCGAG C^TCGAG
1209 CTCGAG
PleI 1261 GAGTC GAGTC(4/5)
1358 GAGTC
PvuII 10 CAGCTG CAG^CTG
1344 CAGCTG
SmlI 91 CTCGAG C^T[CT][GA]AG
1209 CTCGAG
StuI 324 AGGCCT AGG^CCT
1701 AGGCCT
Tsp509I 1571 AATT ^AATT
1748 AATT
XhoI 91 CTCGAG C^TCGAG
1209 CTCGAG
XhoII 1529 AGATCT [GA]^GATC[CT]
1832 AGATCC
XmnI 369 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1270 GAAGGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BalI BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I