Restriction map for
pFN21AE6231
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 618 GCGG CCGC(-3/-1)
AfaI 655 GTAC GT^AC
AvaII 271 GGACC G^G[AT]CC
BamHI 550 GGATCC G^GATCC
BfaI 595 CTAG C^TAG
BglI 538 GCCAGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 155 AGATCT A^GATCT
BsaI 25 GAGACC GGTCTC(1/5)
BsmAI 25 GAGAC GTCTC(1/5)
BsmFI 145 GGGAC GGGAC(10/14)
BsmI 362 GCATTC GAATGC(1/-1)
BsrI 539 CCAGT ACTGG(1/-1)
BstXI 329 CCAAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 271 GGACC G^G[ACGT]CC
DdeI 367 CTGAG C^T[ACGT]AG
DraII 270 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 515 CCACGG C^C[GA][CT]GG
EcoO109I 270 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 586 GAATTC G^AATTC
HaeIII 417 GGCC GG^CC
HincII 324 GTTGAC GT[CT]^[GA]AC
HindII 324 GTTGAC GT[CT]^[GA]AC
HindIII 331 AAGCTT A^AGCTT
MaeI 595 CTAG C^TAG
MseI 424 TTAA T^TAA
NspI 692 GCATGT [GA]CATG^[CT]
PleI 708 GACTC GAGTC(4/5)
PpuMI 270 AGGACCC [GA]G^G[AT]CC[CT]
PstI 439 CTGCAG CTGCA^G
RsaI 655 GTAC GT^AC
Sau96I 271 GGACC G^G[ACGT]CC
SfcI 439 CTGCAG C^T[GA][CT]AG
TfiI 502 GATTC G^A[AT]TC
Tru9I 424 TTAA T^TAA
Tsp45I 232 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 219 AAATTT [GA]^AATT[CT]
586 GAATTC
ApoI 219 AAATTT [GA]^AATT[CT]
586 GAATTC
BbvI 347 GCAGC GCAGC(8/12)
615 GCTGC
BspHI 498 TCATGA T^CATGA
711 TCATGA
BstYI 155 AGATCT [GA]^GATC[CT]
550 GGATCC
Fnu4HI 347 GCAGC GC^[ACGT]GC
615 GCTGC
HinfI 502 GATTC G^A[ACGT]TC
708 GACTC
ItaI 347 GCAGC GC^[ACGT]GC
615 GCTGC
MaeIII 232 GTGAC ^GT[ACGT]AC
674 GTTAC
MboII 111 GAAGA GAAGA(8/7)
727 GAAGA
MflI 155 AGATCT [GA]^GATC[CT]
550 GGATCC
NlaIV 271 GGACCC GG[ACGT]^[ACGT]CC
550 GGATCC
RcaI 498 TCATGA T^CATGA
711 TCATGA
SfaNI 564 GATGC GCATC(5/9)
199 GCATC
TseI 347 GCAGC G^C[AT]GC
615 GCTGC
XcmI 153 CCAGATCTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
533 CCAAAGCCAGTCTGG
XhoII 155 AGATCT [GA]^GATC[CT]
550 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AlwI AspHI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstNI DpnII EcoRII MboI MnlI
MslI MvaI MwoI NdeII NlaIII
Sau3AI ScrFI Tsp509I TspRI