Restriction map for
pFN21AE3928
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# NO CUT ENZYMES ( 148 enzymes) #
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Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsmFI
Bsp1407I BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 231 GACGTC GACGT^C
AcyI 231 GACGTC G[GA]^CG[CT]C
AlwI 601 GGATC GGATC(4/5)
AlwNI 1018 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1014 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 905 GAGCAC G[AT]GC[AT]^C
BanII 961 GAGCCC G[GA]GC[CT]^C
BfaI 890 CTAG C^TAG
BsaHI 231 GACGTC G[GA]^CG[CT]C
BsiHKAI 905 GAGCAC G[AT]GC[AT]^C
BsmI 417 GCATTC GAATGC(1/-1)
BspDI 1063 ATCGAT AT^CGAT
BspMI 665 ACCTGC ACCTGC(4/8)
BsrBI 999 CCGCTC CCGCTC(-3/-3)
BsrDI 429 CATTGC GCAATG(2/0)
BstAPI 907 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 48 TTCGAA TT^CGAA
BstXI 622 CCAATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 549 GCGC GCG^C
ClaI 1063 ATCGAT AT^CGAT
DraII 892 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 892 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 597 CCATGG C^C[AT][AT]GG
EcoT22I 255 ATGCAT ATGCA^T
HhaI 549 GCGC GCG^C
Hin1I 231 GACGTC G[GA]^CG[CT]C
HinP1I 549 GCGC G^CGC
HincII 1070 GTCGAC GT[CT]^[GA]AC
HindII 1070 GTCGAC GT[CT]^[GA]AC
HindIII 295 AAGCTT A^AGCTT
MaeI 890 CTAG C^TAG
MslI 645 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 597 CCATGG C^CATGG
NlaIV 32 GGCCCC GG[ACGT]^[ACGT]CC
NsiI 255 ATGCAT ATGCA^T
NspI 512 ACATGC [GA]CATG^[CT]
NspV 48 TTCGAA TT^CGAA
PaeR7I 564 CTCGAG C^TCGAG
PleI 449 GACTC GAGTC(4/5)
PshAI 227 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1070 GTCGAC G^TCGAC
SapI 610 GCTCTTC GCTCTTC(1/4)
SexAI 954 ACCTGGT A^CC[AT]GGT
SfuI 48 TTCGAA TT^CGAA
SspI 485 AATATT AAT^ATT
StyI 597 CCATGG C^C[AT][AT]GG
Tsp45I 803 GTGAC ^GT[GC]AC
XhoI 564 CTCGAG C^TCGAG
XmnI 1014 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 864 GTCTAC GT^[AC][GT]AC
1070 GTCGAC
AciI 999 CCGC CCGC(-3/-1)
4 GCGG
AcsI 70 AAATTC [GA]^AATT[CT]
405 AAATTT
AfaI 184 GTAC GT^AC
382 GTAC
ApoI 70 AAATTC [GA]^AATT[CT]
405 AAATTT
AvaI 463 CCCGAG C^[CT]CG[GA]G
564 CTCGAG
BciVI 925 GGATAC GTATCC(6/5)
593 GTATCC
BpmI 714 CTCCAG CTGGAG(16/14)
1042 CTCCAG
BsaI 89 GAGACC GGTCTC(1/5)
831 GGTCTC
BseRI 19 GAGGAG GAGGAG(10/8)
979 GAGGAG
BsoBI 463 CCCGAG C^[CT]CG[GA]G
564 CTCGAG
Cfr13I 32 GGCCC G^G[ACGT]CC
893 GGGCC
EarI 611 CTCTTC CTCTTC(1/4)
1120 GAAGAG
Ksp632I 611 CTCTTC CTCTTC(1/4)
1120 GAAGAG
MaeII 137 ACGT A^CGT
232 ACGT
MspA1I 520 CAGCTG C[AC]G^C[GT]G
605 CAGCTG
PstI 222 CTGCAG CTGCA^G
517 CTGCAG
PvuII 520 CAGCTG CAG^CTG
605 CAGCTG
RsaI 184 GTAC GT^AC
382 GTAC
Sau96I 32 GGCCC G^G[ACGT]CC
893 GGGCC
SmlI 564 CTCGAG C^T[CT][GA]AG
1002 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI BbvI BmyI BsaJI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI DpnII DsaI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII Sau3AI
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I