##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AgeI        AhdI        AluI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeIII      HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TfiI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AfaI          188  GTAC                 GT^AC
AflIII        164  ACATGT               A^C[GA][CT]GT
AlwI           63  GATCC                GGATC(4/5)
AvaII         244  GGACC                G^G[AT]CC
BsmFI         131  GTCCC                GGGAC(10/14)
BspLU11I      164  ACATGT               A^CATGT
BstBI         236  TTCGAA               TT^CGAA
BstF5I        127  GGATG                GGATG(2/0)
Cfr13I        244  GGACC                G^G[ACGT]CC
DdeI          110  CTCAG                C^T[ACGT]AG
DpnII          63  GATC                 ^GATC
FokI          127  GGATG                GGATG(9/13)
MboI           63  GATC                 ^GATC
NdeII          63  GATC                 ^GATC
NlaIV         244  GGACCC               GG[ACGT]^[ACGT]CC
NspI          164  ACATGT               [GA]CATG^[CT]
NspV          236  TTCGAA               TT^CGAA
RsaI          188  GTAC                 GT^AC
Sau3AI         63  GATC                 ^GATC
Sau96I        244  GGACC                G^G[ACGT]CC
SfuI          236  TTCGAA               TT^CGAA
SspI          223  AATATT               AAT^ATT
TaqI          237  TCGA                 T^CGA
Tsp45I        161  GTGAC                ^GT[GC]AC
Tsp509I        71  AATT                 ^AATT
TspRI         159  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       237  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BsiYI          67  CCTCAATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              148  CCTGTGTGTGG         
BslI           67  CCTCAATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              148  CCTGTGTGTGG         
BssKI          36  CCTGG                ^CC[ACGT]GG
              124  CCAGG               
BstNI          36  CCTGG                CC^[AT]GG
              124  CCAGG               
EcoRII         36  CCTGG                ^CC[AT]GG
              124  CCAGG               
HaeII           3  GGCGCT               [GA]GCGC^[CT]
               24  GGCGCT              
MaeIII         43  GTTAC                ^GT[ACGT]AC
              161  GTGAC               
MnlI           56  CCTC                 CCTC(7/6)
               67  CCTC                
MseI          143  TTAA                 T^TAA
              227  TTAA                
MvaI           36  CCTGG                CC^[AT]GG
              124  CCAGG               
MwoI            6  GCTGTCGGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               18  GCGCTTGGCGC         
NlaIII        165  CATG                 CATG^
              198  CATG                
ScrFI          36  CCTGG                CC^[ACGT]GG
              124  CCAGG               
Tru9I         143  TTAA                 T^TAA
              227  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BbvI        CfoI        Fnu4HI      HhaI        HinP1I      
ItaI        TseI