Restriction map for
pFN21AE6727
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflIII AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII BamHI
BanI BbeI BbrPI BbvCI BcgI
BcnI BglI BglII BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstUI
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoO109I
EcoRI EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 1591 TCCGGA T^CCGGA
AciI 1472 GCGG CCGC(-3/-1)
AflII 1750 CTTAAG C^TTAAG
AhdI 1361 GACTAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1601 CCTCAGG CC^T[ACGT]AGG
AspEI 1361 GACTAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 703 GGACC G^G[AT]CC
AvrII 1698 CCTAGG C^CTAGG
BalI 2113 TGGCCA TGG^CCA
BciVI 1714 GGATAC GTATCC(6/5)
BfrI 1750 CTTAAG C^TTAAG
BlnI 1698 CCTAGG C^CTAGG
BsaWI 1591 TCCGGA [AT]^CCGG[AT]
BseAI 1591 TCCGGA T^CCGGA
Bsp1407I 1078 TGTACA T^GTACA
BspEI 1591 TCCGGA T^CCGGA
BspHI 1401 TCATGA T^CATGA
BsrGI 1078 TGTACA T^GTACA
Bst1107I 1060 GTATAC GTA^TAC
BstEII 1303 GGTTACC G^GT[ACGT]ACC
BstPI 1303 GGTTACC G^GT[ACGT]ACC
BstXI 164 CCAAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1060 GTATAC GTA^TAC
Bsu36I 1601 CCTCAGG CC^T[ACGT]AGG
EaeI 2113 TGGCCA [CT]^GGCC[GA]
Eam1105I 1361 GACTAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1601 CCTCAGG CC^T[ACGT]AGG
EcoNI 1523 CCTCCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1303 GGTTACC G^GT[ACGT]ACC
EcoRV 223 GATATC GAT^ATC
HapII 1592 CCGG C^CGG
HincII 751 GTTGAC GT[CT]^[GA]AC
HindII 751 GTTGAC GT[CT]^[GA]AC
HpaII 1592 CCGG C^CGG
MaeII 1120 ACGT A^CGT
MluNI 2113 TGGCCA TGG^CCA
MroI 1591 TCCGGA T^CCGGA
MscI 2113 TGGCCA TGG^CCA
MslI 1563 CACTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 863 CAGCTG C[AC]G^C[GT]G
MspI 1592 CCGG C^CGG
MwoI 1501 GCTTGTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 1490 CATATG CA^TATG
PleI 469 GAGTC GAGTC(4/5)
PvuII 863 CAGCTG CAG^CTG
RcaI 1401 TCATGA T^CATGA
SacI 1409 GAGCTC GAGCT^C
ScaI 1977 AGTACT AGT^ACT
SspBI 1078 TGTACA T^GTACA
StuI 64 AGGCCT AGG^CCT
Tsp45I 1117 GTGAC ^GT[GC]AC
XcmI 608 CCATTCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 1060 GTATAC GT^[AC][GT]AC
2052 GTCTAC
Asp700I 62 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1242 GAAAAAATTC
AspHI 192 GAGCAC G[AT]GC[AT]^C
1409 GAGCTC
BanII 1186 GGGCTC G[GA]GC[CT]^C
1409 GAGCTC
BclI 960 TGATCA T^GATCA
2088 TGATCA
BpmI 1164 CTCCAG CTGGAG(16/14)
1811 CTCCAG
BsgI 1560 CTGCAC GTGCAG(16/14)
682 GTGCAG
BsiHKAI 192 GAGCAC G[AT]GC[AT]^C
1409 GAGCTC
BsmFI 1929 GGGAC GGGAC(10/14)
1640 GTCCC
BstYI 798 AGATCC [GA]^GATC[CT]
901 AGATCC
EarI 549 CTCTTC CTCTTC(1/4)
189 GAAGAG
FbaI 960 TGATCA T^GATCA
2088 TGATCA
Ksp632I 549 CTCTTC CTCTTC(1/4)
189 GAAGAG
MflI 798 AGATCC [GA]^GATC[CT]
901 AGATCC
SapI 189 GAAGAGC GCTCTTC(1/4)
548 GCTCTTC
SfaNI 1436 GATGC GCATC(5/9)
449 GCATC
SmlI 1431 CTCAAG C^T[CT][GA]AG
1750 CTTAAG
SspI 1218 AATATT AAT^ATT
1543 AATATT
XhoII 798 AGATCC [GA]^GATC[CT]
901 AGATCC
XmnI 62 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1242 GAAAAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BmyI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI StyI TfiI Tru9I
TseI Tsp509I TspRI Van91I