##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NarI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SfcI        SfiI        SfoI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccI          616  GTAGAC               GT^[AC][GT]AC
AccII         223  CGCG                 CG^CG
AflIII         94  ACATGT               A^C[GA][CT]GT
BcgI          843  CGACTGTCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmBI         308  GAGACG               CGTCTC(1/5)
BspLU11I       94  ACATGT               A^CATGT
BsrBI         384  GAGCGG               CCGCTC(-3/-3)
BsrFI         416  GCCGGC               [GA]^CCGG[CT]
BstBI         801  TTCGAA               TT^CGAA
BstUI         223  CGCG                 CG^CG
Cfr10I        416  GCCGGC               [GA]^CCGG[CT]
DsaI           20  CCATGG               C^C[GA][CT]GG
EcoT14I        20  CCATGG               C^C[AT][AT]GG
HgaI          310  GACGC                GACGC(5/10)
MaeIII        437  GTGAC                ^GT[ACGT]AC
MvnI          223  CGCG                 CG^CG
NaeI          416  GCCGGC               GCC^GGC
NcoI           20  CCATGG               C^CATGG
NgoMIV        416  GCCGGC               G^CCGGC
NspI           94  ACATGT               [GA]CATG^[CT]
NspV          801  TTCGAA               TT^CGAA
PshAI          91  GACACATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         544  CAGCTG               CAG^CTG
SacI          607  GAGCTC               GAGCT^C
SexAI         620  ACCTGGT              A^CC[AT]GGT
SfaNI         291  GATGC                GCATC(5/9)
SfuI          801  TTCGAA               TT^CGAA
StyI           20  CCATGG               C^C[AT][AT]GG
TaqI          802  TCGA                 T^CGA
Tsp45I        437  GTGAC                ^GT[GC]AC
TthHB8I       802  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

ApaI          173  GGGCCC               GGGCC^C
              262  GGGCCC              
AspHI         430  GTGCTC               G[AT]GC[AT]^C
              607  GAGCTC              
BfaI           46  CTAG                 C^TAG
              364  CTAG                
BglI           16  GCCACCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              119  GCCTGGAGGGC         
BseRI         215  GAGGAG               GAGGAG(10/8)
              278  GAGGAG              
BsiHKAI       430  GTGCTC               G[AT]GC[AT]^C
              607  GAGCTC              
BsmFI         509  GGGAC                GGGAC(10/14)
              882  GGGAC               
FauI          973  CCCGC                CCCGC(4/6)
              730  GCGGG               
HphI          105  GGTGA                GGTGA(8/7)
              140  TCACC               
MaeI           46  CTAG                 C^TAG
              364  CTAG                
MboII         764  TCTTC                GAAGA(8/7)
              799  TCTTC               
MspA1I        475  CAGCGG               C[AC]G^C[GT]G
              544  CAGCTG              
PpuMI         356  AGGACCT              [GA]G^G[AT]CC[CT]
              882  GGGACCC             
Tsp509I       736  AATT                 ^AATT
              805  AATT                
TspRI         257  CAGTG                CA[GC]TG[ACGT][ACGT]^
              726  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaI        BsaJI       BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DraII       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HpaII       ItaI        MnlI        
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       SmaI        
TseI        XmaCI       XmaI