Restriction map for
pFN21AE3925
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KasI KspI MamI
MboI MfeI MflI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI Sau3AI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 172 GGTACC G^GTACC
AcyI 181 GACGCC G[GA]^CG[CT]C
AocI 167 CCTCAGG CC^T[ACGT]AGG
Asp718I 172 GGTACC G^GTACC
AvaII 240 GGACC G^G[AT]CC
BanI 172 GGTACC G^G[CT][GA]CC
BbrPI 558 CACGTG CAC^GTG
BcnI 540 CCGGG CC^[GC]GG
BfaI 377 CTAG C^TAG
BsaAI 558 CACGTG [CT]AC^GT[GA]
BsaHI 181 GACGCC G[GA]^CG[CT]C
BsgI 528 GTGCAG GTGCAG(16/14)
Bsp1407I 2 TGTACA T^GTACA
BsrGI 2 TGTACA T^GTACA
BstAPI 22 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 167 CCTCAGG CC^T[ACGT]AGG
CfoI 394 GCGC GCG^C
EarI 129 CTCTTC CTCTTC(1/4)
Eco81I 167 CCTCAGG CC^T[ACGT]AGG
EcoNI 449 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 393 GGCGCT [GA]GCGC^[CT]
HapII 540 CCGG C^CGG
HhaI 394 GCGC GCG^C
Hin1I 181 GACGCC G[GA]^CG[CT]C
HinP1I 394 GCGC G^CGC
HpaII 540 CCGG C^CGG
KpnI 172 GGTACC GGTAC^C
Ksp632I 129 CTCTTC CTCTTC(1/4)
MaeI 377 CTAG C^TAG
MaeII 559 ACGT A^CGT
MboII 130 TCTTC GAAGA(8/7)
MslI 570 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 540 CCGG C^CGG
NciI 540 CCGGG CC^[GC]GG
PmaCI 558 CACGTG CAC^GTG
PmlI 558 CACGTG CAC^GTG
PshAI 181 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 128 GCTCTTC GCTCTTC(1/4)
SspBI 2 TGTACA T^GTACA
StuI 164 AGGCCT AGG^CCT
TaqI 39 TCGA T^CGA
TthHB8I 39 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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AfaI 3 GTAC GT^AC
173 GTAC
AspHI 286 GTGCTC G[AT]GC[AT]^C
385 GAGCAC
BbvCI 111 CCTCAGC CCTCAGC(-5/-2)
303 GCTGAGG
BsiHKAI 286 GTGCTC G[AT]GC[AT]^C
385 GAGCAC
BsmFI 315 GTCCC GGGAC(10/14)
630 GTCCC
HgaI 181 GACGC GACGC(5/10)
590 GACGC
HinfI 399 GAGTC G^A[ACGT]TC
413 GACTC
NlaIII 43 CATG CATG^
576 CATG
PflMI 66 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
443 CCAGCTCCTGG
PleI 413 GACTC GAGTC(4/5)
399 GAGTC
RsaI 3 GTAC GT^AC
173 GTAC
SbfI 19 CCTGCAGG CCTGCA^GG
228 CCTGCAGG
Sse8387I 19 CCTGCAGG CCTGCA^GG
228 CCTGCAGG
Van91I 66 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
443 CCAGCTCCTGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
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AciI AluI AlwNI ApaI BalI
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII ItaI
MaeIII MluNI MnlI MscI MspA1I
MvaI MwoI NlaIV PstI PvuII
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI