##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MwoI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tru9I       Tsp45I      Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccII          91  CGCG                 CG^CG
AcyI          304  GACGCC               G[GA]^CG[CT]C
AlwI           66  GATCC                GGATC(4/5)
Asp700I       111  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          148  GGCACC               G^G[CT][GA]CC
BmyI          231  GGGCAC               G[AGT]GC[ACT]^C
BsaHI         304  GACGCC               G[GA]^CG[CT]C
BsaI          167  GAGACC               GGTCTC(1/5)
BseRI         127  CTCCTC               GAGGAG(10/8)
BsmAI         167  GAGAC                GTCTC(1/5)
Bsp1286I      231  GGGCAC               G[AGT]GC[ACT]^C
BssSI         217  CTCGTG               CACGAG(-5/-1)
BstNI          81  CCAGG                CC^[AT]GG
BstUI          91  CGCG                 CG^CG
Cac8I         254  GCCCGC               GC[ACGT]^[ACGT]GC
Cfr13I        213  GGGCC                G^G[ACGT]CC
DpnII          66  GATC                 ^GATC
DsaI          239  CCGTGG               C^C[GA][CT]GG
EcoRII         81  CCAGG                ^CC[AT]GG
FauI          255  CCCGC                CCCGC(4/6)
HgaI          304  GACGC                GACGC(5/10)
Hin1I         304  GACGCC               G[GA]^CG[CT]C
HinfI         104  GAATC                G^A[ACGT]TC
MaeII         235  ACGT                 A^CGT
MboI           66  GATC                 ^GATC
MslI          234  CACGTCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        208  CAGCTG               C[AC]G^C[GT]G
MvaI           81  CCAGG                CC^[AT]GG
MvnI           91  CGCG                 CG^CG
NdeII          66  GATC                 ^GATC
NlaIV         148  GGCACC               GG[ACGT]^[ACGT]CC
NspI          161  ACATGC               [GA]CATG^[CT]
PvuII         208  CAGCTG               CAG^CTG
Sau3AI         66  GATC                 ^GATC
Sau96I        213  GGGCC                G^G[ACGT]CC
SfaNI          32  GCATC                GCATC(5/9)
TfiI          104  GAATC                G^A[AT]TC
TspRI          99  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmnI          111  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI          188  AGCT                 AG^CT
              209  AGCT                
BbvI          207  GCAGC                GCAGC(8/12)
              297  GCAGC               
BsrI           98  CCAGT                ACTGG(1/-1)
              180  CCAGT               
BstF5I        276  CATCC                GGATG(2/0)
              291  CATCC               
FokI          276  CATCC                GGATG(9/13)
              291  CATCC               
MboII          15  GAAGA                GAAGA(8/7)
               18  GAAGA               
NlaIII        162  CATG                 CATG^
              267  CATG                
TaqI           56  TCGA                 T^CGA
              119  TCGA                
TseI          207  GCAGC                G^C[AT]GC
              297  GCAGC               
TthHB8I        56  TCGA                 T^CGA
              119  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AciI        AfaI        BcnI        BsaJI       BsiYI       
BslI        BssKI       Fnu4HI      HaeIII      HapII       
HpaII       ItaI        MnlI        MspI        NciI        
RsaI        ScrFI