Restriction map for
pFN21AE4443
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BciVI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1240 GGTACC G^GTACC
AccI 1028 GTCGAC GT^[AC][GT]AC
AcsI 760 GAATTC [GA]^AATT[CT]
Alw44I 700 GTGCAC G^TGCAC
ApaLI 700 GTGCAC G^TGCAC
ApoI 760 GAATTC [GA]^AATT[CT]
Asp700I 57 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1240 GGTACC G^GTACC
AspHI 700 GTGCAC G[AT]GC[AT]^C
AvaI 1229 CTCGGG C^[CT]CG[GA]G
BanI 1240 GGTACC G^G[CT][GA]CC
BanII 739 GGGCTC G[GA]GC[CT]^C
BbvCI 1089 GCTGAGG CCTCAGC(-5/-2)
BglI 263 GCCCATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 890 AGATCT A^GATCT
BsaAI 1106 TACGTA [CT]AC^GT[GA]
BsaWI 458 ACCGGA [AT]^CCGG[AT]
BsiHKAI 700 GTGCAC G[AT]GC[AT]^C
BsmBI 656 GAGACG CGTCTC(1/5)
BsoBI 1229 CTCGGG C^[CT]CG[GA]G
BspHI 764 TCATGA T^CATGA
BsrI 195 CCAGT ACTGG(1/-1)
BstAPI 1332 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 597 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 804 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 760 GAATTC G^AATTC
HgaI 1137 GACGC GACGC(5/10)
KpnI 1240 GGTACC GGTAC^C
MaeII 1107 ACGT A^CGT
MslI 555 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 781 CAGCGG C[AC]G^C[GT]G
PflMI 571 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1121 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 764 TCATGA T^CATGA
SalI 1028 GTCGAC G^TCGAC
ScaI 215 AGTACT AGT^ACT
SexAI 570 ACCAGGT A^CC[AT]GGT
SfcI 995 CTGTAG C^T[GA][CT]AG
SnaBI 1106 TACGTA TAC^GTA
SphI 513 GCATGC GCATG^C
TaqI 1029 TCGA T^CGA
TthHB8I 1029 TCGA T^CGA
Van91I 571 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 57 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 188 CGCG CG^CG
1139 CGCG
AlwNI 1120 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1283 CAGAAACTG
BclI 2 TGATCA T^GATCA
630 TGATCA
BpmI 16 CTGGAG CTGGAG(16/14)
169 CTGGAG
BseRI 865 GAGGAG GAGGAG(10/8)
1315 GAGGAG
BsmAI 656 GAGAC GTCTC(1/5)
1144 GAGAC
BstUI 188 CGCG CG^CG
1139 CGCG
BstYI 890 AGATCT [GA]^GATC[CT]
1278 AGATCC
EaeI 732 CGGCCA [CT]^GGCC[GA]
745 CGGCCA
FbaI 2 TGATCA T^GATCA
630 TGATCA
HindIII 274 AAGCTT A^AGCTT
1294 AAGCTT
MaeIII 911 GTGAC ^GT[ACGT]AC
1135 GTGAC
MflI 890 AGATCT [GA]^GATC[CT]
1278 AGATCC
MvnI 188 CGCG CG^CG
1139 CGCG
NcoI 265 CCATGG C^CATGG
735 CCATGG
NspI 513 GCATGC [GA]CATG^[CT]
1115 ACATGC
Tsp45I 911 GTGAC ^GT[GC]AC
1135 GTGAC
XhoII 890 AGATCT [GA]^GATC[CT]
1278 AGATCC
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI Tsp509I TspRI