Restriction map for
pFN21AE9655
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspDI
BspHI BspLU11I BspMI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MboI MflI MluI
MluNI MscI NarI NciI NcoI
NdeI NdeII NheI NlaIV NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 144 CGCG CG^CG
AccIII 28 TCCGGA T^CCGGA
AflIII 135 ACGTGT A^C[GA][CT]GT
AhdI 120 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 120 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 375 GAAGAC GAAGAC(2/6)
BbvI 166 GCAGC GCAGC(8/12)
BfaI 116 CTAG C^TAG
BpmI 175 CTGGAG CTGGAG(16/14)
BpuAI 375 GAAGAC GAAGAC(2/6)
BsaWI 28 TCCGGA [AT]^CCGG[AT]
BseAI 28 TCCGGA T^CCGGA
Bsp1407I 158 TGTACA T^GTACA
BspEI 28 TCCGGA T^CCGGA
BsrBI 57 CCGCTC CCGCTC(-3/-3)
BsrFI 70 GCCGGC [GA]^CCGG[CT]
BsrGI 158 TGTACA T^GTACA
BssKI 174 CCTGG ^CC[ACGT]GG
BstAPI 208 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 174 CCTGG CC^[AT]GG
BstUI 144 CGCG CG^CG
Cfr10I 70 GCCGGC [GA]^CCGG[CT]
Cfr13I 90 GGGCC G^G[ACGT]CC
Eam1105I 120 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 174 CCTGG ^CC[AT]GG
EcoT22I 215 ATGCAT ATGCA^T
FauI 56 CCCGC CCCGC(4/6)
HphI 185 TCACC GGTGA(8/7)
MaeI 116 CTAG C^TAG
MaeII 135 ACGT A^CGT
MaeIII 254 GTCAC ^GT[ACGT]AC
MfeI 311 CAATTG C^AATTG
MroI 28 TCCGGA T^CCGGA
MseI 229 TTAA T^TAA
MslI 130 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 152 CCGCTG C[AC]G^C[GT]G
MunI 311 CAATTG C^AATTG
MvaI 174 CCTGG CC^[AT]GG
MvnI 144 CGCG CG^CG
NaeI 70 GCCGGC GCC^GGC
NgoMIV 70 GCCGGC G^CCGGC
NlaIII 130 CATG CATG^
NsiI 215 ATGCAT ATGCA^T
PleI 25 GACTC GAGTC(4/5)
Sau96I 90 GGGCC G^G[ACGT]CC
ScrFI 174 CCTGG CC^[ACGT]GG
SfaNI 217 GCATC GCATC(5/9)
SspBI 158 TGTACA T^GTACA
TaqI 102 TCGA T^CGA
TfiI 292 GATTC G^A[AT]TC
Tru9I 229 TTAA T^TAA
TseI 166 GCAGC G^C[AT]GC
Tsp45I 254 GTCAC ^GT[GC]AC
TspRI 86 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 102 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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BstF5I 171 CATCC GGATG(2/0)
339 CATCC
CfoI 143 GCGC GCG^C
164 GCGC
Fnu4HI 151 GCCGC GC^[ACGT]GC
166 GCAGC
FokI 171 CATCC GGATG(9/13)
339 CATCC
HaeIII 36 GGCC GG^CC
91 GGCC
HapII 29 CCGG C^CGG
71 CCGG
HhaI 143 GCGC GCG^C
164 GCGC
HinP1I 143 GCGC G^CGC
164 GCGC
HinfI 25 GACTC G^A[ACGT]TC
292 GATTC
HpaII 29 CCGG C^CGG
71 CCGG
ItaI 151 GCCGC GC^[ACGT]GC
166 GCAGC
MspI 29 CCGG C^CGG
71 CCGG
Tsp509I 287 AATT ^AATT
312 AATT
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AciI AfaI BsrI Cac8I MboII
MnlI MwoI RsaI