Restriction map for
pFN21AE1331
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglII BlnI BpmI BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DpnII DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeII MboI MfeI MflI
MluI MroI MseI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 823 ACATGT A^C[GA][CT]GT
AlwNI 838 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 25 GAGCTC G[AT]GC[AT]^C
BalI 557 TGGCCA TGG^CCA
BciVI 215 GTATCC GTATCC(6/5)
BglI 832 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 413 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 413 GCTCAGC GC^T[ACGT]AGC
BsaBI 726 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 907 CTCCTC GAGGAG(10/8)
BsiHKAI 25 GAGCTC G[AT]GC[AT]^C
BsmAI 116 GAGAC GTCTC(1/5)
BsmFI 484 GTCCC GGGAC(10/14)
BspLU11I 823 ACATGT A^CATGT
BstBI 64 TTCGAA TT^CGAA
CelII 413 GCTCAGC GC^T[ACGT]AGC
DraIII 886 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 557 TGGCCA [CT]^GGCC[GA]
EcoRI 78 GAATTC G^AATTC
FauI 935 CCCGC CCCGC(4/6)
HincII 819 GTCAAC GT[CT]^[GA]AC
HindII 819 GTCAAC GT[CT]^[GA]AC
MamI 726 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
MslI 330 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 223 GCTAGC G^CTAGC
NspI 823 ACATGT [GA]CATG^[CT]
NspV 64 TTCGAA TT^CGAA
PflMI 462 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 482 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 25 GAGCTC GAGCT^C
SfcI 632 CTGTAG C^T[GA][CT]AG
SfuI 64 TTCGAA TT^CGAA
SmlI 48 CTCAAG C^T[CT][GA]AG
StuI 489 AGGCCT AGG^CCT
TaqI 65 TCGA T^CGA
TfiI 100 GATTC G^A[AT]TC
Tsp45I 550 GTCAC ^GT[GC]AC
TthHB8I 65 TCGA T^CGA
Van91I 462 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 78 GAATTC [GA]^AATT[CT]
515 GAATTT
ApoI 78 GAATTC [GA]^AATT[CT]
515 GAATTT
BanII 25 GAGCTC G[GA]GC[CT]^C
502 GGGCTC
BspMI 164 ACCTGC ACCTGC(4/8)
848 ACCTGC
BstXI 203 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
715 CCAGGCCCCTGG
DdeI 41 CTGAG C^T[ACGT]AG
414 CTCAG
DraII 482 AGGTCCC [GA]G^G[ACGT]CC[CT]
717 AGGCCCC
EcoO109I 482 AGGTCCC [GA]G^G[ACGT]CC[CT]
717 AGGCCCC
EcoT14I 683 CCAAGG C^C[AT][AT]GG
993 CCTTGG
MaeIII 161 GTAAC ^GT[ACGT]AC
550 GTCAC
PleI 175 GACTC GAGTC(4/5)
578 GACTC
StyI 683 CCAAGG C^C[AT][AT]GG
993 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BfaI BmyI BsaJI BsgI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MboII MnlI
MspA1I MvaI MwoI NlaIII NlaIV
PvuII Sau96I ScrFI TseI Tsp509I
TspRI