Restriction map for
pFN21AE1339
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
Ksp632I KspI MaeII MamI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI ScaI SfiI SgrAI
SnaBI SpeI SspI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1321 GGTACC G^GTACC
AccI 400 GTAGAC GT^[AC][GT]AC
AcyI 451 GGCGCC G[GA]^CG[CT]C
AhdI 268 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1106 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1321 GGTACC G^GTACC
AspEI 268 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1179 TGGCCA TGG^CCA
BbeI 451 GGCGCC GGCGC^C
BbvCI 262 GCTGAGG CCTCAGC(-5/-2)
BfaI 136 CTAG C^TAG
BglI 535 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1439 AGATCT A^GATCT
BsaHI 451 GGCGCC G[GA]^CG[CT]C
Bsp1407I 1412 TGTACA T^GTACA
BspHI 1515 TCATGA T^CATGA
BsrDI 73 GCAATG GCAATG(2/0)
BsrGI 1412 TGTACA T^GTACA
BssSI 1528 CACGAG CACGAG(-5/-1)
BstBI 85 TTCGAA TT^CGAA
BstF5I 425 GGATG GGATG(2/0)
DsaI 482 CCATGG C^C[GA][CT]GG
EaeI 1179 TGGCCA [CT]^GGCC[GA]
Eam1105I 268 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 315 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 425 GGATG GGATG(9/13)
Hin1I 451 GGCGCC G[GA]^CG[CT]C
KasI 451 GGCGCC G^GCGCC
KpnI 1321 GGTACC GGTAC^C
MaeI 136 CTAG C^TAG
MluNI 1179 TGGCCA TGG^CCA
MscI 1179 TGGCCA TGG^CCA
NarI 451 GGCGCC GG^CGCC
NcoI 482 CCATGG C^CATGG
NspV 85 TTCGAA TT^CGAA
PshAI 751 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1515 TCATGA T^CATGA
SacI 31 GAGCTC GAGCT^C
SexAI 861 ACCAGGT A^CC[AT]GGT
SfoI 451 GGCGCC GGC^GCC
SfuI 85 TTCGAA TT^CGAA
SmaI 1015 CCCGGG CCC^GGG
SspBI 1412 TGTACA T^GTACA
StuI 1035 AGGCCT AGG^CCT
Tsp45I 839 GTGAC ^GT[GC]AC
XcmI 536 CCAAGGGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1015 CCCGGG C^CCGGG
XmaI 1015 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 215 CGCG CG^CG
370 CGCG
AcsI 14 AAATTC [GA]^AATT[CT]
1356 AAATTC
AlwI 159 GATCC GGATC(4/5)
1207 GGATC
ApaI 927 GGGCCC GGGCC^C
1406 GGGCCC
ApoI 14 AAATTC [GA]^AATT[CT]
1356 AAATTC
AvaI 573 CTCGGG C^[CT]CG[GA]G
1015 CCCGGG
BbsI 461 GTCTTC GAAGAC(2/6)
1372 GTCTTC
BpuAI 461 GTCTTC GAAGAC(2/6)
1372 GTCTTC
BseRI 111 GAGGAG GAGGAG(10/8)
1531 GAGGAG
BsoBI 573 CTCGGG C^[CT]CG[GA]G
1015 CCCGGG
BstAPI 339 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
384 GCACTTGCTGC
BstUI 215 CGCG CG^CG
370 CGCG
BstYI 1207 GGATCT [GA]^GATC[CT]
1439 AGATCT
HaeII 175 GGCGCT [GA]GCGC^[CT]
451 GGCGCC
MflI 1207 GGATCT [GA]^GATC[CT]
1439 AGATCT
MslI 483 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
949 CACCCAGGTG
MspA1I 170 CCGCTG C[AC]G^C[GT]G
394 CAGCGG
MvnI 215 CGCG CG^CG
370 CGCG
NspI 488 GCATGC [GA]CATG^[CT]
1382 GCATGC
PleI 596 GAGTC GAGTC(4/5)
1020 GAGTC
SbfI 345 CCTGCAGG CCTGCA^GG
1111 CCTGCAGG
SmlI 19 CTCAAG C^T[CT][GA]AG
36 CTTGAG
SphI 488 GCATGC GCATG^C
1382 GCATGC
Sse8387I 345 CCTGCAGG CCTGCA^GG
1111 CCTGCAGG
TaqI 86 TCGA T^CGA
331 TCGA
TthHB8I 86 TCGA T^CGA
331 TCGA
XhoII 1207 GGATCT [GA]^GATC[CT]
1439 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp509I TspRI