Restriction map for
pFN21AE4442
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbsI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 1218 GAATTC [GA]^AATT[CT]
AcyI 800 GGCGCC G[GA]^CG[CT]C
AfaI 657 GTAC GT^AC
ApaI 843 GGGCCC GGGCC^C
ApoI 1218 GAATTC [GA]^AATT[CT]
AspHI 712 GAGCAC G[AT]GC[AT]^C
AviII 584 TGCGCA TGC^GCA
BalI 594 TGGCCA TGG^CCA
BamHI 225 GGATCC G^GATCC
BanI 800 GGCGCC G^G[CT][GA]CC
BbeI 800 GGCGCC GGCGC^C
BbrPI 751 CACGTG CAC^GTG
BsaAI 751 CACGTG [CT]AC^GT[GA]
BsaHI 800 GGCGCC G[GA]^CG[CT]C
BsaI 986 GGTCTC GGTCTC(1/5)
BsiHKAI 712 GAGCAC G[AT]GC[AT]^C
BspMI 694 GCAGGT ACCTGC(4/8)
BsrBI 565 CCGCTC CCGCTC(-3/-3)
BstAPI 69 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoNI 49 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1218 GAATTC G^AATTC
EcoRV 493 GATATC GAT^ATC
FspI 584 TGCGCA TGC^GCA
Hin1I 800 GGCGCC G[GA]^CG[CT]C
HincII 784 GTTGAC GT[CT]^[GA]AC
HindII 784 GTTGAC GT[CT]^[GA]AC
KasI 800 GGCGCC G^GCGCC
MaeII 752 ACGT A^CGT
MluNI 594 TGGCCA TGG^CCA
MscI 594 TGGCCA TGG^CCA
NarI 800 GGCGCC GG^CGCC
PflMI 17 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 751 CACGTG CAC^GTG
PmlI 751 CACGTG CAC^GTG
RsaI 657 GTAC GT^AC
SapI 155 GCTCTTC GCTCTTC(1/4)
SexAI 1095 ACCTGGT A^CC[AT]GGT
SfiI 816 GGCCGTGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 800 GGCGCC GGC^GCC
SmlI 365 CTCAAG C^T[CT][GA]AG
Tsp509I 1219 AATT ^AATT
Van91I 17 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 468 CGCG CG^CG
1287 CGCG
AlwNI 18 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
871 CAGTTGCTG
BbvCI 762 CCTCAGC CCTCAGC(-5/-2)
898 GCTGAGG
BfaI 628 CTAG C^TAG
1246 CTAG
BlpI 1057 GCTAAGC GC^T[ACGT]AGC
1201 GCTGAGC
Bpu1102I 1057 GCTAAGC GC^T[ACGT]AGC
1201 GCTGAGC
BstUI 468 CGCG CG^CG
1287 CGCG
CelII 1057 GCTAAGC GC^T[ACGT]AGC
1201 GCTGAGC
EcoT14I 143 CCTTGG C^C[AT][AT]GG
794 CCTTGG
HaeII 422 GGCGCT [GA]GCGC^[CT]
800 GGCGCC
HgaI 1131 GACGC GACGC(5/10)
1283 GCGTC
MaeI 628 CTAG C^TAG
1246 CTAG
MseI 293 TTAA T^TAA
1034 TTAA
MvnI 468 CGCG CG^CG
1287 CGCG
PstI 667 CTGCAG CTGCA^G
692 CTGCAG
StyI 143 CCTTGG C^C[AT][AT]GG
794 CCTTGG
TfiI 136 GATTC G^A[AT]TC
208 GATTC
Tru9I 293 TTAA T^TAA
1034 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIV
PleI PpuMI PshAI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI TseI Tsp45I TspRI XhoII
XmaCI XmaI