Restriction map for
pFN21AE3618
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
BalI BamHI BcgI BciVI BclI
BfrI BglII BlpI BpmI Bpu1102I
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 590 GGTACC G^GTACC
AcyI 208 GGCGCC G[GA]^CG[CT]C
AfaI 591 GTAC GT^AC
AlwI 162 GATCC GGATC(4/5)
Aor51HI 83 AGCGCT AGC^GCT
Asp718I 590 GGTACC G^GTACC
AspI 481 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 313 CCCGAG C^[CT]CG[GA]G
AviII 878 TGCGCA TGC^GCA
AvrII 117 CCTAGG C^CTAGG
BbeI 208 GGCGCC GGCGC^C
BbrPI 148 CACGTG CAC^GTG
BbsI 550 GAAGAC GAAGAC(2/6)
BbvCI 301 GCTGAGG CCTCAGC(-5/-2)
BfaI 118 CTAG C^TAG
BlnI 117 CCTAGG C^CTAGG
BpuAI 550 GAAGAC GAAGAC(2/6)
BsaAI 148 CACGTG [CT]AC^GT[GA]
BsaHI 208 GGCGCC G[GA]^CG[CT]C
BsoBI 313 CCCGAG C^[CT]CG[GA]G
BspMI 130 ACCTGC ACCTGC(4/8)
BsrBI 96 CCGCTC CCGCTC(-3/-3)
BstAPI 755 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 161 AGATCC [GA]^GATC[CT]
DpnII 162 GATC ^GATC
DraIII 490 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 83 AGCGCT AGC^GCT
EcoT14I 117 CCTAGG C^C[AT][AT]GG
FspI 878 TGCGCA TGC^GCA
Hin1I 208 GGCGCC G[GA]^CG[CT]C
HinfI 504 GACTC G^A[ACGT]TC
KasI 208 GGCGCC G^GCGCC
KpnI 590 GGTACC GGTAC^C
MaeI 118 CTAG C^TAG
MboI 162 GATC ^GATC
MflI 161 AGATCC [GA]^GATC[CT]
MslI 148 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 331 GCCGGC GCC^GGC
NarI 208 GGCGCC GG^CGCC
NdeII 162 GATC ^GATC
NgoMIV 331 GCCGGC G^CCGGC
NspI 429 GCATGC [GA]CATG^[CT]
PflFI 481 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 513 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 504 GACTC GAGTC(4/5)
PmaCI 148 CACGTG CAC^GTG
PmlI 148 CACGTG CAC^GTG
RsaI 591 GTAC GT^AC
Sau3AI 162 GATC ^GATC
SexAI 806 ACCTGGT A^CC[AT]GGT
SfoI 208 GGCGCC GGC^GCC
SmlI 691 CTTGAG C^T[CT][GA]AG
SphI 429 GCATGC GCATG^C
StuI 120 AGGCCT AGG^CCT
StyI 117 CCTAGG C^C[AT][AT]GG
Tth111I 481 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 513 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 161 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BglI 559 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
670 GCCCAGAGGGC
BsaWI 464 TCCGGT [AT]^CCGG[AT]
546 ACCGGA
BsgI 28 CTGCAC GTGCAG(16/14)
496 GTGCAG
BsmAI 479 GAGAC GTCTC(1/5)
502 GAGAC
BsrFI 61 GCCGGT [GA]^CCGG[CT]
331 GCCGGC
BstF5I 153 GGATG GGATG(2/0)
711 GGATG
BstXI 147 CCACGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
707 CCAGGGATGTGG
Cfr10I 61 GCCGGT [GA]^CCGG[CT]
331 GCCGGC
DsaI 33 CCGTGG C^C[GA][CT]GG
535 CCGTGG
EaeI 289 TGGCCG [CT]^GGCC[GA]
801 CGGCCA
EarI 196 GAAGAG CTCTTC(1/4)
476 GAAGAG
FauI 95 CCCGC CCCGC(4/6)
407 GCGGG
FokI 153 GGATG GGATG(9/13)
711 GGATG
HaeII 83 AGCGCT [GA]GCGC^[CT]
208 GGCGCC
HgaI 553 GACGC GACGC(5/10)
267 GCGTC
HphI 64 GGTGA GGTGA(8/7)
363 GGTGA
Ksp632I 196 GAAGAG CTCTTC(1/4)
476 GAAGAG
MaeII 149 ACGT A^CGT
751 ACGT
NlaIII 249 CATG CATG^
430 CATG
PpuMI 3 GGGACCT [GA]G^G[AT]CC[CT]
530 GGGACCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EcoNI EcoO109I EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV PstI
PvuII Sau96I ScrFI SfcI TseI
TspRI