Restriction map for
pFN21AE4581
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# NO CUT ENZYMES ( 75 enzymes) #
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AclI AflII AgeI AscI AseI
AsnI AvaI AviII AvrII BbvCI
BcgI BciVI BfaI BfrI BlnI
BsaBI BsaI BsiWI BsmI BsoBI
Bsp1407I BspDI BsrDI BsrGI Bst1107I
BstZ17I ClaI CpoI DraI DrdI
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KspI MaeI MamI
MfeI MluI MseI MunI NheI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SfaNI SfiI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI Tru9I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1783 GACGTC GACGT^C
Acc65I 2366 GGTACC G^GTACC
AccI 1486 GTCTAC GT^[AC][GT]AC
AccIII 356 TCCGGA T^CCGGA
AcsI 1014 GAATTT [GA]^AATT[CT]
AhdI 1957 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2196 GTGCAC G^TGCAC
Aor51HI 374 AGCGCT AGC^GCT
ApaI 2285 GGGCCC GGGCC^C
ApaLI 2196 GTGCAC G^TGCAC
ApoI 1014 GAATTT [GA]^AATT[CT]
Asp718I 2366 GGTACC G^GTACC
AspEI 1957 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 859 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BamHI 401 GGATCC G^GATCC
BclI 683 TGATCA T^GATCA
BglII 821 AGATCT A^GATCT
BlpI 1950 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1950 GCTGAGC GC^T[ACGT]AGC
BseAI 356 TCCGGA T^CCGGA
BspEI 356 TCCGGA T^CCGGA
BspLU11I 1043 ACATGT A^CATGT
BssHII 133 GCGCGC G^CGCGC
BstBI 1205 TTCGAA TT^CGAA
CelII 1950 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1957 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 374 AGCGCT AGC^GCT
FbaI 683 TGATCA T^GATCA
HincII 795 GTCAAC GT[CT]^[GA]AC
HindII 795 GTCAAC GT[CT]^[GA]AC
KpnI 2366 GGTACC GGTAC^C
MluNI 2 TGGCCA TGG^CCA
MroI 356 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
NaeI 1574 GCCGGC GCC^GGC
NdeI 172 CATATG CA^TATG
NgoMIV 1574 GCCGGC G^CCGGC
NspV 1205 TTCGAA TT^CGAA
PflFI 859 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2395 GAGCTC GAGCT^C
SapI 1413 GAAGAGC GCTCTTC(1/4)
ScaI 830 AGTACT AGT^ACT
SexAI 2303 ACCTGGT A^CC[AT]GGT
SfuI 1205 TTCGAA TT^CGAA
SphI 1995 GCATGC GCATG^C
StuI 1065 AGGCCT AGG^CCT
Tsp509I 1015 AATT ^AATT
Tth111I 859 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AflIII 1043 ACATGT A^C[GA][CT]GT
2174 ACGTGT
AocI 1911 CCTTAGG CC^T[ACGT]AGG
2115 CCTGAGG
Asp700I 957 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2124 GAAGGACTTC
BbeI 60 GGCGCC GGCGC^C
2111 GGCGCC
BbrPI 658 CACGTG CAC^GTG
2173 CACGTG
BglI 1264 GCCGTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2013 GCCTGCCCGGC
BsaAI 658 CACGTG [CT]AC^GT[GA]
2173 CACGTG
BsaWI 356 TCCGGA [AT]^CCGG[AT]
694 TCCGGT
BsgI 1840 CTGCAC GTGCAG(16/14)
1361 GTGCAG
BsiEI 33 CGGCCG CG[GA][CT]^CG
881 CGGCCG
BspMI 1906 ACCTGC ACCTGC(4/8)
1236 GCAGGT
BstAPI 871 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1824 GCAGAGTGTGC
BstEII 1857 GGTCACC G^GT[ACGT]ACC
2343 GGTGACC
BstPI 1857 GGTCACC G^GT[ACGT]ACC
2343 GGTGACC
Bsu36I 1911 CCTTAGG CC^T[ACGT]AGG
2115 CCTGAGG
EagI 33 CGGCCG C^GGCCG
881 CGGCCG
EarI 1429 CTCTTC CTCTTC(1/4)
1413 GAAGAG
EclXI 33 CGGCCG C^GGCCG
881 CGGCCG
Eco52I 33 CGGCCG C^GGCCG
881 CGGCCG
Eco81I 1911 CCTTAGG CC^T[ACGT]AGG
2115 CCTGAGG
EcoNI 321 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1907 CCTGCCTTAGG
EcoO65I 1857 GGTCACC G^GT[ACGT]ACC
2343 GGTGACC
KasI 60 GGCGCC G^GCGCC
2111 GGCGCC
Ksp632I 1429 CTCTTC CTCTTC(1/4)
1413 GAAGAG
NarI 60 GGCGCC GG^CGCC
2111 GGCGCC
PleI 1090 GACTC GAGTC(4/5)
164 GAGTC
PmaCI 658 CACGTG CAC^GTG
2173 CACGTG
PmlI 658 CACGTG CAC^GTG
2173 CACGTG
SbfI 1233 CCTGCAGG CCTGCA^GG
1320 CCTGCAGG
SfoI 60 GGCGCC GGC^GCC
2111 GGCGCC
Sse8387I 1233 CCTGCAGG CCTGCA^GG
1320 CCTGCAGG
TfiI 614 GAATC G^A[AT]TC
1208 GAATC
XmnI 957 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2124 GAAGGACTTC
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# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspHI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII