Restriction map for
pFN21AE1414
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KspI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 620 GGTACC G^GTACC
AccII 21 CGCG CG^CG
AlwNI 185 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 620 GGTACC G^GTACC
AvaI 69 CCCGGG C^[CT]CG[GA]G
BbsI 376 GTCTTC GAAGAC(2/6)
BbvCI 109 CCTCAGC CCTCAGC(-5/-2)
BcgI 392 GCAACTACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 316 CTAG C^TAG
BglI 66 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 574 GCTCAGC GC^T[ACGT]AGC
BpmI 156 CTCCAG CTGGAG(16/14)
Bpu1102I 574 GCTCAGC GC^T[ACGT]AGC
BpuAI 376 GTCTTC GAAGAC(2/6)
BsaWI 212 TCCGGT [AT]^CCGG[AT]
BsoBI 69 CCCGGG C^[CT]CG[GA]G
BstAPI 184 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 565 GGTTACC G^GT[ACGT]ACC
BstPI 565 GGTTACC G^GT[ACGT]ACC
BstUI 21 CGCG CG^CG
CelII 574 GCTCAGC GC^T[ACGT]AGC
EaeI 176 CGGCCA [CT]^GGCC[GA]
EarI 294 CTCTTC CTCTTC(1/4)
EcoO65I 565 GGTTACC G^GT[ACGT]ACC
EcoT14I 422 CCTTGG C^C[AT][AT]GG
FauI 19 CCCGC CCCGC(4/6)
KpnI 620 GGTACC GGTAC^C
Ksp632I 294 CTCTTC CTCTTC(1/4)
MaeI 316 CTAG C^TAG
MaeIII 566 GTTAC ^GT[ACGT]AC
MvnI 21 CGCG CG^CG
PleI 292 GACTC GAGTC(4/5)
PstI 612 CTGCAG CTGCA^G
SfcI 612 CTGCAG C^T[GA][CT]AG
SmaI 69 CCCGGG CCC^GGG
SphI 85 GCATGC GCATG^C
StyI 422 CCTTGG C^C[AT][AT]GG
TaqI 401 TCGA T^CGA
TthHB8I 401 TCGA T^CGA
XcmI 456 CCATCTGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 69 CCCGGG C^CCGGG
XmaI 69 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 357 GGTCC G^G[AT]CC
414 GGACC
BanI 607 GGCACC G^G[CT][GA]CC
620 GGTACC
BanII 9 GAGCCC G[GA]GC[CT]^C
403 GAGCCC
BmyI 9 GAGCCC G[AGT]GC[ACT]^C
403 GAGCCC
BsgI 182 GTGCAG GTGCAG(16/14)
427 GTGCAG
Bsp1286I 9 GAGCCC G[AGT]GC[ACT]^C
403 GAGCCC
BstF5I 381 CATCC GGATG(2/0)
264 GGATG
DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
413 AGGACCT
EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
413 AGGACCT
FokI 381 CATCC GGATG(9/13)
264 GGATG
HincII 601 GTTGAC GT[CT]^[GA]AC
655 GTTGAC
HindII 601 GTTGAC GT[CT]^[GA]AC
655 GTTGAC
HphI 79 GGTGA GGTGA(8/7)
479 TCACC
MboII 295 TCTTC GAAGA(8/7)
377 TCTTC
MspA1I 90 CAGCTG C[AC]G^C[GT]G
615 CAGCTG
NlaIII 86 CATG CATG^
598 CATG
NspI 85 GCATGC [GA]CATG^[CT]
597 GCATGT
PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT]
413 AGGACCT
PvuII 90 CAGCTG CAG^CTG
615 CAGCTG
TfiI 141 GATTC G^A[AT]TC
444 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BsaJI BsiYI BslI BspMI BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MnlI MspI MvaI MwoI NciI
NlaIV RsaI Sau96I ScrFI TseI
TspRI