Restriction map for
pFN21AE6430
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvrII
BbeI BbrPI BbsI BciVI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT22I FseI HaeII Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspA1I MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SwaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 1386 CGCG CG^CG
AcsI 994 GAATTC [GA]^AATT[CT]
AflIII 1045 ACATGT A^C[GA][CT]GT
AlwNI 1114 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 994 GAATTC [GA]^AATT[CT]
Asp700I 1636 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1229 TGCGCA TGC^GCA
BalI 1248 TGGCCA TGG^CCA
BamHI 1413 GGATCC G^GATCC
BanI 1510 GGTGCC G^G[CT][GA]CC
BanII 1174 GAGCTC G[GA]GC[CT]^C
BcgI 1266 GCAGGACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 872 TGATCA T^GATCA
BlpI 319 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 319 GCTAAGC GC^T[ACGT]AGC
BseRI 187 GAGGAG GAGGAG(10/8)
BsgI 936 GTGCAG GTGCAG(16/14)
Bsp1407I 1048 TGTACA T^GTACA
BspLU11I 1045 ACATGT A^CATGT
BspMI 1490 GCAGGT ACCTGC(4/8)
BsrGI 1048 TGTACA T^GTACA
BstUI 1386 CGCG CG^CG
CelII 319 GCTAAGC GC^T[ACGT]AGC
CfoI 1230 GCGC GCG^C
DsaI 1682 CCATGG C^C[GA][CT]GG
EaeI 1248 TGGCCA [CT]^GGCC[GA]
EcoRI 994 GAATTC G^AATTC
EcoRV 667 GATATC GAT^ATC
FbaI 872 TGATCA T^GATCA
FspI 1229 TGCGCA TGC^GCA
HgaI 988 GACGC GACGC(5/10)
HhaI 1230 GCGC GCG^C
HinP1I 1230 GCGC G^CGC
HincII 1422 GTCAAC GT[CT]^[GA]AC
HindII 1422 GTCAAC GT[CT]^[GA]AC
MboII 1836 GAAGA GAAGA(8/7)
MluNI 1248 TGGCCA TGG^CCA
MscI 1248 TGGCCA TGG^CCA
MslI 1626 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1386 CGCG CG^CG
NcoI 1682 CCATGG C^CATGG
NspI 1045 ACATGT [GA]CATG^[CT]
PaeR7I 1396 CTCGAG C^TCGAG
SacI 1174 GAGCTC GAGCT^C
SbfI 1540 CCTGCAGG CCTGCA^GG
ScaI 827 AGTACT AGT^ACT
SexAI 611 ACCAGGT A^CC[AT]GGT
SmaI 1754 CCCGGG CCC^GGG
Sse8387I 1540 CCTGCAGG CCTGCA^GG
SspBI 1048 TGTACA T^GTACA
StuI 483 AGGCCT AGG^CCT
Tsp45I 599 GTGAC ^GT[GC]AC
XbaI 1362 TCTAGA T^CTAGA
XcmI 1673 CCAAAAAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1396 CTCGAG C^TCGAG
XmaCI 1754 CCCGGG C^CCGGG
XmaI 1754 CCCGGG C^CCGGG
XmnI 1636 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 1174 GAGCTC G[AT]GC[AT]^C
1393 GTGCTC
AvaI 1396 CTCGAG C^[CT]CG[GA]G
1754 CCCGGG
BbvCI 310 GCTGAGG CCTCAGC(-5/-2)
715 GCTGAGG
BmyI 1174 GAGCTC G[AGT]GC[ACT]^C
1393 GTGCTC
BsaI 1106 GAGACC GGTCTC(1/5)
145 GGTCTC
BsiHKAI 1174 GAGCTC G[AT]GC[AT]^C
1393 GTGCTC
BsoBI 1396 CTCGAG C^[CT]CG[GA]G
1754 CCCGGG
Bsp1286I 1174 GAGCTC G[AGT]GC[ACT]^C
1393 GTGCTC
DraIII 67 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
956 CACATGGTG
EcoT14I 1343 CCAAGG C^C[AT][AT]GG
1682 CCATGG
HphI 1074 GGTGA GGTGA(8/7)
1625 TCACC
MaeII 476 ACGT A^CGT
707 ACGT
MaeIII 218 GTTAC ^GT[ACGT]AC
599 GTGAC
PflMI 612 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1793 CCAAAGCATGG
SmlI 1396 CTCGAG C^T[CT][GA]AG
1605 CTTGAG
SspI 435 AATATT AAT^ATT
457 AATATT
StyI 1343 CCAAGG C^C[AT][AT]GG
1682 CCATGG
TaqI 1397 TCGA T^CGA
1737 TCGA
TthHB8I 1397 TCGA T^CGA
1737 TCGA
Van91I 612 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1793 CCAAAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AspI
AvaII BbvI BcnI BfaI BpmI
BsaJI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeI MboI MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflFI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp509I TspRI Tth111I XhoII