Restriction map for
pFN21AE1124
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AspI
AviII AvrII BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI MamI MluI MroI
MseI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 818 GGTACC G^GTACC
AcsI 793 AAATTT [GA]^AATT[CT]
AhdI 460 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1205 GTGCAC G^TGCAC
ApaLI 1205 GTGCAC G^TGCAC
ApoI 793 AAATTT [GA]^AATT[CT]
Asp718I 818 GGTACC G^GTACC
AspEI 460 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1025 TGGCCA TGG^CCA
BamHI 147 GGATCC G^GATCC
BbeI 1305 GGCGCC GGCGC^C
BbrPI 1174 CACGTG CAC^GTG
BfaI 130 CTAG C^TAG
BglI 229 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 773 TCCGGT [AT]^CCGG[AT]
BseRI 1227 CTCCTC GAGGAG(10/8)
BsrFI 316 GCCGGC [GA]^CCGG[CT]
BssSI 427 CACGAG CACGAG(-5/-1)
Cfr10I 316 GCCGGC [GA]^CCGG[CT]
DraIII 855 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 460 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 308 CCATGG C^C[AT][AT]GG
HgaI 375 GCGTC GACGC(5/10)
KasI 1305 GGCGCC G^GCGCC
KpnI 818 GGTACC GGTAC^C
KspI 1238 CCGCGG CCGC^GG
MaeI 130 CTAG C^TAG
MfeI 138 CAATTG C^AATTG
MluNI 1025 TGGCCA TGG^CCA
MscI 1025 TGGCCA TGG^CCA
MunI 138 CAATTG C^AATTG
NaeI 316 GCCGGC GCC^GGC
NarI 1305 GGCGCC GG^CGCC
NcoI 308 CCATGG C^CATGG
NgoMIV 316 GCCGGC G^CCGGC
NspI 617 ACATGC [GA]CATG^[CT]
PaeR7I 1381 CTCGAG C^TCGAG
PmaCI 1174 CACGTG CAC^GTG
PmlI 1174 CACGTG CAC^GTG
PpuMI 302 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 979 GAGCTC GAGCT^C
SacII 1238 CCGCGG CCGC^GG
SfoI 1305 GGCGCC GGC^GCC
SmlI 1381 CTCGAG C^T[CT][GA]AG
StyI 308 CCATGG C^C[AT][AT]GG
XhoI 1381 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 374 GGCGTC G[GA]^CG[CT]C
1305 GGCGCC
BanII 54 GAGCCC G[GA]GC[CT]^C
979 GAGCTC
BcnI 406 CCGGG CC^[GC]GG
1328 CCGGG
BsaAI 1174 CACGTG [CT]AC^GT[GA]
1294 TACGTG
BsaHI 374 GGCGTC G[GA]^CG[CT]C
1305 GGCGCC
BsiEI 1325 CGGCCG CG[GA][CT]^CG
1340 CGGCCG
BsmFI 1092 GGGAC GGGAC(10/14)
304 GTCCC
BspMI 108 ACCTGC ACCTGC(4/8)
1038 GCAGGT
BsrBI 754 CCGCTC CCGCTC(-3/-3)
441 GAGCGG
BstAPI 632 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1005 GCAGGCCCTGC
DraII 302 AGGTCCC [GA]G^G[ACGT]CC[CT]
1007 AGGCCCT
EagI 1325 CGGCCG C^GGCCG
1340 CGGCCG
EarI 682 CTCTTC CTCTTC(1/4)
535 GAAGAG
EclXI 1325 CGGCCG C^GGCCG
1340 CGGCCG
Eco52I 1325 CGGCCG C^GGCCG
1340 CGGCCG
EcoO109I 302 AGGTCCC [GA]G^G[ACGT]CC[CT]
1007 AGGCCCT
HaeII 660 GGCGCT [GA]GCGC^[CT]
1305 GGCGCC
Hin1I 374 GGCGTC G[GA]^CG[CT]C
1305 GGCGCC
Ksp632I 682 CTCTTC CTCTTC(1/4)
535 GAAGAG
MslI 387 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
390 CATTGTGGTG
NciI 406 CCGGG CC^[GC]GG
1328 CCGGG
PflMI 882 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1170 CCATCACGTGG
PleI 460 GACTC GAGTC(4/5)
841 GACTC
PstI 1198 CTGCAG CTGCA^G
1351 CTGCAG
TaqI 839 TCGA T^CGA
1382 TCGA
TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^
1310 CAGTG
TthHB8I 839 TCGA T^CGA
1382 TCGA
Van91I 882 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1170 CCATCACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI TseI
Tsp45I Tsp509I XhoII