Restriction map for
pFN21AE9574
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsgI BsiHKAI BsiWI
BsmBI BsmFI BsoBI BspDI BspHI
BspMI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MseI MspA1I MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AluI 839 AGCT AG^CT
AlwI 461 GGATC GGATC(4/5)
Asp700I 3 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 428 GGTGCC G^G[CT][GA]CC
BciVI 51 GGATAC GTATCC(6/5)
BglI 448 GCCGGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 34 GGGCAC G[AGT]GC[ACT]^C
BpmI 802 CTGGAG CTGGAG(16/14)
BsaAI 898 TACGTG [CT]AC^GT[GA]
BsiEI 396 CGACCG CG[GA][CT]^CG
BsmAI 856 GTCTC GTCTC(1/5)
BsmI 187 GCATTC GAATGC(1/-1)
Bsp1286I 34 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 288 TGTACA T^GTACA
BspLU11I 356 ACATGT A^CATGT
BsrDI 161 GCAATG GCAATG(2/0)
BsrGI 288 TGTACA T^GTACA
DpnII 462 GATC ^GATC
DrdI 969 GACTGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 973 GAAGAG CTCTTC(1/4)
EcoT14I 119 CCTTGG C^C[AT][AT]GG
FseI 906 GGCCGGCC GGCCGG^CC
HincII 966 GTTGAC GT[CT]^[GA]AC
HindII 966 GTTGAC GT[CT]^[GA]AC
Ksp632I 973 GAAGAG CTCTTC(1/4)
MboI 462 GATC ^GATC
NdeII 462 GATC ^GATC
NlaIII 357 CATG CATG^
NspI 356 ACATGT [GA]CATG^[CT]
PstI 324 CTGCAG CTGCA^G
Sau3AI 462 GATC ^GATC
ScaI 539 AGTACT AGT^ACT
SexAI 773 ACCAGGT A^CC[AT]GGT
SfiI 447 GGCCGGCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 721 CTCAAG C^T[CT][GA]AG
SspBI 288 TGTACA T^GTACA
StyI 119 CCTTGG C^C[AT][AT]GG
TaqI 191 TCGA T^CGA
Tsp509I 13 AATT ^AATT
TthHB8I 191 TCGA T^CGA
XmnI 3 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 704 CGCG CG^CG
1007 CGCG
AccIII 680 TCCGGA T^CCGGA
956 TCCGGA
AflIII 356 ACATGT A^C[GA][CT]GT
962 ACGTGT
BbsI 1035 GAAGAC GAAGAC(2/6)
139 GTCTTC
BcnI 522 CCGGG CC^[GC]GG
531 CCGGG
BpuAI 1035 GAAGAC GAAGAC(2/6)
139 GTCTTC
BsaWI 680 TCCGGA [AT]^CCGG[AT]
956 TCCGGA
BseAI 680 TCCGGA T^CCGGA
956 TCCGGA
BseRI 952 CTCCTC GAGGAG(10/8)
535 GAGGAG
BspEI 680 TCCGGA T^CCGGA
956 TCCGGA
BsrFI 448 GCCGGC [GA]^CCGG[CT]
907 GCCGGC
BsrI 302 ACTGG ACTGG(1/-1)
746 CCAGT
BstUI 704 CGCG CG^CG
1007 CGCG
Cfr10I 448 GCCGGC [GA]^CCGG[CT]
907 GCCGGC
FauI 314 GCGGG CCCGC(4/6)
591 GCGGG
HaeII 261 GGCGCT [GA]GCGC^[CT]
391 AGCGCC
HinfI 844 GACTC G^A[ACGT]TC
976 GAGTC
MroI 680 TCCGGA T^CCGGA
956 TCCGGA
MvnI 704 CGCG CG^CG
1007 CGCG
NaeI 448 GCCGGC GCC^GGC
907 GCCGGC
NciI 522 CCGGG CC^[GC]GG
531 CCGGG
NgoMIV 448 GCCGGC G^CCGGC
907 GCCGGC
PleI 844 GACTC GAGTC(4/5)
976 GAGTC
SfcI 324 CTGCAG C^T[GA][CT]AG
573 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AvaII BalI BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DsaI
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboII MluNI
MnlI MscI MslI MspI MvaI
MwoI NlaIV RsaI Sau96I ScrFI
SfaNI TseI Tsp45I TspRI XcmI