Restriction map for
pFN21AE1440
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsiEI
BsiWI BsmAI BsmBI BspDI BspLU11I
BspMI BsrBI BssHII Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MscI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 55 GTCTAC GT^[AC][GT]AC
AccIII 62 TCCGGA T^CCGGA
AcyI 169 GACGCC G[GA]^CG[CT]C
Alw44I 624 GTGCAC G^TGCAC
AlwNI 903 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 624 GTGCAC G^TGCAC
AvaI 506 CCCGGG C^[CT]CG[GA]G
BanI 862 GGCACC G^G[CT][GA]CC
BanII 286 GAGCTC G[GA]GC[CT]^C
BbsI 274 GTCTTC GAAGAC(2/6)
BglI 337 GCCGGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 574 CTGGAG CTGGAG(16/14)
BpuAI 274 GTCTTC GAAGAC(2/6)
BsaHI 169 GACGCC G[GA]^CG[CT]C
BseAI 62 TCCGGA T^CCGGA
BsmFI 428 GGGAC GGGAC(10/14)
BsmI 522 GAATGC GAATGC(1/-1)
BsoBI 506 CCCGGG C^[CT]CG[GA]G
BspEI 62 TCCGGA T^CCGGA
BspHI 536 TCATGA T^CATGA
BssSI 621 CTCGTG CACGAG(-5/-1)
BstAPI 1007 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 846 GGTGACC G^GT[ACGT]ACC
BstPI 846 GGTGACC G^GT[ACGT]ACC
DraII 839 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 607 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 237 TGGCCG [CT]^GGCC[GA]
EcoO109I 839 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 846 GGTGACC G^GT[ACGT]ACC
HaeII 17 GGCGCT [GA]GCGC^[CT]
HgaI 169 GACGC GACGC(5/10)
Hin1I 169 GACGCC G[GA]^CG[CT]C
HinfI 531 GACTC G^A[ACGT]TC
MroI 62 TCCGGA T^CCGGA
MslI 1026 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 788 CCATGG C^CATGG
PleI 531 GACTC GAGTC(4/5)
PpuMI 839 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 211 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 359 CAGCTG CAG^CTG
RcaI 536 TCATGA T^CATGA
SacI 286 GAGCTC GAGCT^C
SexAI 800 ACCTGGT A^CC[AT]GGT
SgrAI 336 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 506 CCCGGG CCC^GGG
SmlI 682 CTCAAG C^T[CT][GA]AG
StuI 126 AGGCCT AGG^CCT
TaqI 812 TCGA T^CGA
TthHB8I 812 TCGA T^CGA
XcmI 488 CCAAGGTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 506 CCCGGG C^CCGGG
XmaI 506 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1010 ACACGT A^C[GA][CT]GT
1037 ACACGT
BcgI 269 GCACCGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
476 GCATCGGCTTCG
BsaWI 5 ACCGGA [AT]^CCGG[AT]
62 TCCGGA
BsgI 254 CTGCAC GTGCAG(16/14)
1014 GTGCAG
Bsp1407I 806 TGTACA T^GTACA
1034 TGTACA
BsrDI 330 CATTGC GCAATG(2/0)
233 GCAATG
BsrGI 806 TGTACA T^GTACA
1034 TGTACA
BstF5I 465 CATCC GGATG(2/0)
501 GGATG
BstYI 119 AGATCC [GA]^GATC[CT]
569 AGATCC
DsaI 606 CCACGG C^C[GA][CT]GG
788 CCATGG
EcoT14I 488 CCAAGG C^C[AT][AT]GG
788 CCATGG
FauI 956 CCCGC CCCGC(4/6)
144 GCGGG
FokI 465 CATCC GGATG(9/13)
501 GGATG
HphI 846 GGTGA GGTGA(8/7)
317 TCACC
MboII 756 GAAGA GAAGA(8/7)
275 TCTTC
MflI 119 AGATCC [GA]^GATC[CT]
569 AGATCC
NaeI 161 GCCGGC GCC^GGC
337 GCCGGC
NgoMIV 161 GCCGGC G^CCGGC
337 GCCGGC
SspBI 806 TGTACA T^GTACA
1034 TGTACA
StyI 488 CCAAGG C^C[AT][AT]GG
788 CCATGG
Tsp45I 847 GTGAC ^GT[GC]AC
911 GTGAC
XhoII 119 AGATCC [GA]^GATC[CT]
569 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BbrPI BbvI BcnI
BmyI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MaeIII MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PmaCI PmlI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI