Restriction map for
pFN21AB7783
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BamHI BanII BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII KpnI MluI MluNI
MroI MscI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RcaI RsrII SacI
SalI SbfI ScaI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI TaqI TthHB8I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 358 GTAGAC GT^[AC][GT]AC
AlwI 705 GGATC GGATC(4/5)
AocI 1179 CCTGAGG CC^T[ACGT]AGG
AspI 250 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 292 CCTCAGC CCTCAGC(-5/-2)
BcnI 139 CCCGG CC^[GC]GG
BfaI 709 CTAG C^TAG
BglII 645 AGATCT A^GATCT
BlpI 15 GCTGAGC GC^T[ACGT]AGC
BmyI 43 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC
BsaBI 646 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 462 GAGACC GGTCTC(1/5)
BsiEI 1246 CGATCG CG[GA][CT]^CG
Bsp1286I 43 GTGCCC G[AGT]GC[ACT]^C
BsrDI 742 GCAATG GCAATG(2/0)
BstEII 386 GGTTACC G^GT[ACGT]ACC
BstPI 386 GGTTACC G^GT[ACGT]ACC
Bsu36I 1179 CCTGAGG CC^T[ACGT]AGG
CelII 15 GCTGAGC GC^T[ACGT]AGC
DraI 1167 TTTAAA TTT^AAA
Eco81I 1179 CCTGAGG CC^T[ACGT]AGG
EcoO65I 386 GGTTACC G^GT[ACGT]ACC
EcoT14I 776 CCATGG C^C[AT][AT]GG
HincII 1045 GTTAAC GT[CT]^[GA]AC
HindII 1045 GTTAAC GT[CT]^[GA]AC
HpaI 1045 GTTAAC GTT^AAC
KspI 232 CCGCGG CCGC^GG
MaeI 709 CTAG C^TAG
MaeII 410 ACGT A^CGT
MamI 646 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 992 CAATTG C^AATTG
MunI 992 CAATTG C^AATTG
NciI 139 CCCGG CC^[GC]GG
NcoI 776 CCATGG C^CATGG
NdeI 1351 CATATG CA^TATG
NspI 839 GCATGC [GA]CATG^[CT]
PflFI 250 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1185 GACTC GAGTC(4/5)
PstI 818 CTGCAG CTGCA^G
PvuI 1246 CGATCG CGAT^CG
PvuII 770 CAGCTG CAG^CTG
SacII 232 CCGCGG CCGC^GG
SapI 132 GCTCTTC GCTCTTC(1/4)
SexAI 251 ACCTGGT A^CC[AT]GGT
SfcI 818 CTGCAG C^T[GA][CT]AG
SgrAI 53 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 88 CTCAAG C^T[CT][GA]AG
SphI 839 GCATGC GCATG^C
SspI 613 AATATT AAT^ATT
StuI 783 AGGCCT AGG^CCT
StyI 776 CCATGG C^C[AT][AT]GG
TfiI 111 GAATC G^A[AT]TC
Tsp45I 906 GTCAC ^GT[GC]AC
Tth111I 250 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 708 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 960 GAATTT [GA]^AATT[CT]
1023 AAATTT
AfaI 684 GTAC GT^AC
1214 GTAC
ApoI 960 GAATTT [GA]^AATT[CT]
1023 AAATTT
Asp700I 408 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
825 GAAACAATTC
BbsI 412 GTCTTC GAAGAC(2/6)
1158 GTCTTC
BpuAI 412 GTCTTC GAAGAC(2/6)
1158 GTCTTC
BsaWI 702 ACCGGA [AT]^CCGG[AT]
1271 ACCGGA
BseRI 533 CTCCTC GAGGAG(10/8)
145 GAGGAG
BsmAI 341 GAGAC GTCTC(1/5)
462 GAGAC
BsmFI 36 GGGAC GGGAC(10/14)
661 GTCCC
BstYI 645 AGATCT [GA]^GATC[CT]
705 GGATCT
DsaI 232 CCGCGG C^C[GA][CT]GG
776 CCATGG
EarI 133 CTCTTC CTCTTC(1/4)
1356 GAAGAG
HgaI 124 GACGC GACGC(5/10)
1109 GACGC
HinfI 111 GAATC G^A[ACGT]TC
1185 GACTC
Ksp632I 133 CTCTTC CTCTTC(1/4)
1356 GAAGAG
MflI 645 AGATCT [GA]^GATC[CT]
705 GGATCT
MspA1I 232 CCGCGG C[AC]G^C[GT]G
770 CAGCTG
NaeI 54 GCCGGC GCC^GGC
207 GCCGGC
NgoMIV 54 GCCGGC G^CCGGC
207 GCCGGC
PpuMI 36 GGGACCC [GA]G^G[AT]CC[CT]
248 AGGACCT
RsaI 684 GTAC GT^AC
1214 GTAC
SfaNI 951 GATGC GCATC(5/9)
1112 GCATC
XhoII 645 AGATCT [GA]^GATC[CT]
705 GGATCT
XmnI 408 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
825 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaII BanI BbeI BbvI BpmI
BsaHI BsaJI BsiYI BslI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MvnI MwoI NarI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfoI
Tru9I TseI Tsp509I TspRI