Restriction map for
pFN21AE1369
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AclI AcsI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII BamHI BbrPI
BbsI BcgI BclI BfrI BglII
BpuAI BsaAI BsaBI BsaI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrGI BssHII BssSI BstBI
BstEII BstPI BstXI Bsu36I ClaI
CpoI DraI DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
FbaI FseI FspI HincII HindII
HindIII HpaI Ksp632I MaeII MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 824 GGTACC G^GTACC
AccI 1345 GTATAC GT^[AC][GT]AC
AccIII 951 TCCGGA T^CCGGA
AgeI 868 ACCGGT A^CCGGT
AlwI 1239 GATCC GGATC(4/5)
AlwNI 1057 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 824 GGTACC G^GTACC
AvrII 130 CCTAGG C^CTAGG
BalI 1398 TGGCCA TGG^CCA
BbvCI 351 CCTCAGC CCTCAGC(-5/-2)
BciVI 690 GTATCC GTATCC(6/5)
BlnI 130 CCTAGG C^CTAGG
BseAI 951 TCCGGA T^CCGGA
BsgI 1388 GTGCAG GTGCAG(16/14)
BsiEI 45 CGGCCG CG[GA][CT]^CG
BsmAI 759 GAGAC GTCTC(1/5)
BsmBI 759 GAGACG CGTCTC(1/5)
BspEI 951 TCCGGA T^CCGGA
Bst1107I 1345 GTATAC GTA^TAC
BstYI 1238 AGATCC [GA]^GATC[CT]
BstZ17I 1345 GTATAC GTA^TAC
DpnII 1239 GATC ^GATC
EagI 45 CGGCCG C^GGCCG
EclXI 45 CGGCCG C^GGCCG
Eco52I 45 CGGCCG C^GGCCG
EcoRV 1446 GATATC GAT^ATC
EcoT22I 1284 ATGCAT ATGCA^T
HinfI 61 GAATC G^A[ACGT]TC
KpnI 824 GGTACC GGTAC^C
MboI 1239 GATC ^GATC
MflI 1238 AGATCC [GA]^GATC[CT]
MluNI 1398 TGGCCA TGG^CCA
MroI 951 TCCGGA T^CCGGA
MscI 1398 TGGCCA TGG^CCA
MslI 492 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 1239 GATC ^GATC
NsiI 1284 ATGCAT ATGCA^T
NspI 1571 ACATGC [GA]CATG^[CT]
PflMI 1056 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 868 ACCGGT A^CCGGT
PstI 471 CTGCAG CTGCA^G
Sau3AI 1239 GATC ^GATC
SexAI 936 ACCAGGT A^CC[AT]GGT
SfiI 957 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 867 CACCGGTG C[GA]^CCGG[CT]G
TfiI 61 GAATC G^A[AT]TC
Tsp509I 1372 AATT ^AATT
Van91I 1056 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 23 CCAGTCGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1238 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BlpI 214 GCTGAGC GC^T[ACGT]AGC
646 GCTCAGC
Bpu1102I 214 GCTGAGC GC^T[ACGT]AGC
646 GCTCAGC
BsaWI 868 ACCGGT [AT]^CCGG[AT]
951 TCCGGA
BseRI 149 GAGGAG GAGGAG(10/8)
928 GAGGAG
BsrDI 1046 GCAATG GCAATG(2/0)
1249 GCAATG
CelII 214 GCTGAGC GC^T[ACGT]AGC
646 GCTCAGC
DraIII 784 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1080 CACCCAGTG
HgaI 211 GACGC GACGC(5/10)
391 GACGC
MboII 1165 GAAGA GAAGA(8/7)
1236 GAAGA
NaeI 186 GCCGGC GCC^GGC
464 GCCGGC
NcoI 1451 CCATGG C^CATGG
1560 CCATGG
NgoMIV 186 GCCGGC G^CCGGC
464 GCCGGC
PvuII 1196 CAGCTG CAG^CTG
1504 CAGCTG
SacI 616 GAGCTC GAGCT^C
716 GAGCTC
SfcI 471 CTGCAG C^T[GA][CT]AG
1134 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI KasI KspI MaeI
MaeIII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NlaIII
NlaIV PpuMI RsaI SacII Sau96I
ScrFI SfaNI SfoI SmaI StyI
TseI Tsp45I TspRI XmaCI XmaI