Restriction map for
pFN21AE3652
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeII MamI MfeI MflI MluI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 132 CGCG CG^CG
AccIII 756 TCCGGA T^CCGGA
AlwI 836 GGATC GGATC(4/5)
AocI 625 CCTGAGG CC^T[ACGT]AGG
Asp700I 79 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 179 GAAGAC GAAGAC(2/6)
BbvCI 316 GCTGAGG CCTCAGC(-5/-2)
BfaI 454 CTAG C^TAG
BglI 307 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 614 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 614 GCTCAGC GC^T[ACGT]AGC
BpuAI 179 GAAGAC GAAGAC(2/6)
BsaI 722 GGTCTC GGTCTC(1/5)
BsaWI 756 TCCGGA [AT]^CCGG[AT]
BseAI 756 TCCGGA T^CCGGA
BspEI 756 TCCGGA T^CCGGA
BspMI 610 ACCTGC ACCTGC(4/8)
BsrFI 765 ACCGGC [GA]^CCGG[CT]
BstAPI 9 GCACCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 135 GGTGACC G^GT[ACGT]ACC
BstPI 135 GGTGACC G^GT[ACGT]ACC
BstUI 132 CGCG CG^CG
Bsu36I 625 CCTGAGG CC^T[ACGT]AGG
CelII 614 GCTCAGC GC^T[ACGT]AGC
Cfr10I 765 ACCGGC [GA]^CCGG[CT]
DpnII 837 GATC ^GATC
DrdI 154 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 625 CCTGAGG CC^T[ACGT]AGG
EcoO65I 135 GGTGACC G^GT[ACGT]ACC
FauI 130 CCCGC CCCGC(4/6)
HgaI 692 GACGC GACGC(5/10)
HinfI 161 GAGTC G^A[ACGT]TC
KspI 131 CCGCGG CCGC^GG
MaeI 454 CTAG C^TAG
MboI 837 GATC ^GATC
MroI 756 TCCGGA T^CCGGA
MvnI 132 CGCG CG^CG
NdeII 837 GATC ^GATC
PleI 161 GAGTC GAGTC(4/5)
PstI 745 CTGCAG CTGCA^G
SacII 131 CCGCGG CCGC^GG
SapI 18 GCTCTTC GCTCTTC(1/4)
Sau3AI 837 GATC ^GATC
SfcI 745 CTGCAG C^T[GA][CT]AG
SmaI 74 CCCGGG CCC^GGG
XmaCI 74 CCCGGG C^CCGGG
XmaI 74 CCCGGG C^CCGGG
XmnI 79 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 608 GTAC GT^AC
843 GTAC
AflIII 338 ACATGT A^C[GA][CT]GT
1014 ACATGT
AvaI 74 CCCGGG C^[CT]CG[GA]G
220 CCCGAG
BalI 89 TGGCCA TGG^CCA
542 TGGCCA
BpmI 64 CTGGAG CTGGAG(16/14)
158 CTGGAG
BseRI 229 GAGGAG GAGGAG(10/8)
319 GAGGAG
BsoBI 74 CCCGGG C^[CT]CG[GA]G
220 CCCGAG
BspLU11I 338 ACATGT A^CATGT
1014 ACATGT
BstXI 296 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
497 CCATGTCTTTGG
CfoI 594 GCGC GCG^C
738 GCGC
DraIII 38 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
668 CACCCAGTG
DsaI 131 CCGCGG C^C[GA][CT]GG
485 CCGTGG
HhaI 594 GCGC GCG^C
738 GCGC
HinP1I 594 GCGC G^CGC
738 GCGC
MluNI 89 TGGCCA TGG^CCA
542 TGGCCA
MscI 89 TGGCCA TGG^CCA
542 TGGCCA
PpuMI 152 GGGACCC [GA]G^G[AT]CC[CT]
480 AGGACCC
RsaI 608 GTAC GT^AC
843 GTAC
SfaNI 115 GATGC GCATC(5/9)
268 GATGC
StuI 456 AGGCCT AGG^CCT
751 AGGCCT
XcmI 632 CCACGCCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
864 CCACTACTGTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaII
BanII BbvI BcnI BmyI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI Ksp632I MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV NspI PflMI PvuII
Sau96I ScrFI StyI TseI Tsp45I
TspRI Van91I