Restriction map for
pFN21AE6720
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BanII
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BlnI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI Bst1107I BstZ17I CelII
ClaI CpoI Eam1105I Eco47III EcoNI
EcoRV FauI FbaI FseI FspI
HgaI HpaI MluI MroI NciI
NdeI NheI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SalI SapI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI SspBI StuI SwaI Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AcsI 660 GAATTC [GA]^AATT[CT]
AcyI 139 GGCGCC G[GA]^CG[CT]C
AflIII 283 ACATGT A^C[GA][CT]GT
ApoI 660 GAATTC [GA]^AATT[CT]
AspHI 1183 GTGCTC G[AT]GC[AT]^C
AvaI 155 CTCGAG C^[CT]CG[GA]G
BbeI 139 GGCGCC GGCGC^C
BbsI 1083 GAAGAC GAAGAC(2/6)
BglI 37 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1083 GAAGAC GAAGAC(2/6)
BsaBI 75 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 139 GGCGCC G[GA]^CG[CT]C
BsgI 2137 GTGCAG GTGCAG(16/14)
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsiHKAI 1183 GTGCTC G[AT]GC[AT]^C
BsmFI 1486 GTCCC GGGAC(10/14)
BsoBI 155 CTCGAG C^[CT]CG[GA]G
BspHI 1764 TCATGA T^CATGA
BspLU11I 283 ACATGT A^CATGT
BsrBI 152 CCGCTC CCGCTC(-3/-3)
BsrFI 62 GCCGGC [GA]^CCGG[CT]
BstAPI 988 GCAGGTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 888 GGTAACC G^GT[ACGT]ACC
BstPI 888 GGTAACC G^GT[ACGT]ACC
BstXI 425 CCATTTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 62 GCCGGC [GA]^CCGG[CT]
DraI 484 TTTAAA TTT^AAA
DraII 1877 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 936 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1879 GACCCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 35 CGGCCG C^GGCCG
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
EcoO109I 1877 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 888 GGTAACC G^GT[ACGT]ACC
EcoRI 660 GAATTC G^AATTC
EcoT22I 16 ATGCAT ATGCA^T
Hin1I 139 GGCGCC G[GA]^CG[CT]C
HincII 2004 GTCAAC GT[CT]^[GA]AC
HindII 2004 GTCAAC GT[CT]^[GA]AC
HindIII 1825 AAGCTT A^AGCTT
KasI 139 GGCGCC G^GCGCC
KspI 38 CCGCGG CCGC^GG
MamI 75 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1856 CAATTG C^AATTG
MspA1I 38 CCGCGG C[AC]G^C[GT]G
MunI 1856 CAATTG C^AATTG
NaeI 62 GCCGGC GCC^GGC
NarI 139 GGCGCC GG^CGCC
NgoMIV 62 GCCGGC G^CCGGC
NotI 34 GCGGCCGC GC^GGCCGC
NruI 11 TCGCGA TCG^CGA
NsiI 16 ATGCAT ATGCA^T
PaeR7I 155 CTCGAG C^TCGAG
PpuMI 1877 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1490 CTGCAG CTGCA^G
RcaI 1764 TCATGA T^CATGA
SacII 38 CCGCGG CCGC^GG
SbfI 1489 CCTGCAGG CCTGCA^GG
SfoI 139 GGCGCC GGC^GCC
Sse8387I 1489 CCTGCAGG CCTGCA^GG
SspI 463 AATATT AAT^ATT
Tsp45I 934 GTCAC ^GT[GC]AC
XhoI 155 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 496 GTAGAC GT^[AC][GT]AC
1351 GTAGAC
AocI 1657 CCTCAGG CC^T[ACGT]AGG
1726 CCTGAGG
Asp700I 1627 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2246 GAAACTGTTC
AvaII 112 GGTCC G^G[AT]CC
1878 GGACC
BalI 262 TGGCCA TGG^CCA
1505 TGGCCA
BglII 405 AGATCT A^GATCT
1227 AGATCT
BmyI 872 GTGCCC G[AGT]GC[ACT]^C
1183 GTGCTC
BpmI 253 CTCCAG CTGGAG(16/14)
605 CTGGAG
Bsp1286I 872 GTGCCC G[AGT]GC[ACT]^C
1183 GTGCTC
BspMI 988 GCAGGT ACCTGC(4/8)
1207 GCAGGT
BsrDI 1989 CATTGC GCAATG(2/0)
370 GCAATG
BstF5I 1662 GGATG GGATG(2/0)
1959 GGATG
Bsu36I 1657 CCTCAGG CC^T[ACGT]AGG
1726 CCTGAGG
EarI 1672 CTCTTC CTCTTC(1/4)
1933 GAAGAG
Eco81I 1657 CCTCAGG CC^T[ACGT]AGG
1726 CCTGAGG
FokI 1662 GGATG GGATG(9/13)
1959 GGATG
HapII 63 CCGG C^CGG
84 CCGG
HpaII 63 CCGG C^CGG
84 CCGG
Ksp632I 1672 CTCTTC CTCTTC(1/4)
1933 GAAGAG
MaeII 1087 ACGT A^CGT
2061 ACGT
MaeIII 889 GTAAC ^GT[ACGT]AC
934 GTCAC
MluNI 262 TGGCCA TGG^CCA
1505 TGGCCA
MscI 262 TGGCCA TGG^CCA
1505 TGGCCA
MspI 63 CCGG C^CGG
84 CCGG
NcoI 563 CCATGG C^CATGG
2073 CCATGG
NspI 283 ACATGT [GA]CATG^[CT]
524 ACATGC
XcmI 458 CCATGAATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1952 CCATGAAGGATGTGG
XmnI 1627 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2246 GAAACTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI Asp718I BanI BbvI BfaI
BsaJI BseRI BsiYI BslI BsmAI
BsmI BsrI BssKI BstBI BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI KpnI
MaeI MboI MboII MflI MnlI
MseI MslI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII