Restriction map for
pFN21AE6409
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 872 GTCTAC GT^[AC][GT]AC
AccII 1098 CGCG CG^CG
AcsI 289 AAATTT [GA]^AATT[CT]
AlwI 1366 GATCC GGATC(4/5)
ApoI 289 AAATTT [GA]^AATT[CT]
Asp700I 226 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1396 GGACC G^G[AT]CC
AvrII 1122 CCTAGG C^CTAGG
BanI 649 GGCACC G^G[CT][GA]CC
BbvI 619 GCTGC GCAGC(8/12)
BcnI 710 CCCGG CC^[GC]GG
BfaI 1123 CTAG C^TAG
BlnI 1122 CCTAGG C^CTAGG
BsaBI 679 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 369 CCAATGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 369 CCAATGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 615 GAATGC GAATGC(1/-1)
BspHI 659 TCATGA T^CATGA
BsrBI 1073 CCGCTC CCGCTC(-3/-3)
BsrDI 731 GCAATG GCAATG(2/0)
BsrI 1374 ACTGG ACTGG(1/-1)
BstUI 1098 CGCG CG^CG
BstYI 1365 AGATCC [GA]^GATC[CT]
Cfr13I 1396 GGACC G^G[ACGT]CC
FauI 1072 CCCGC CCCGC(4/6)
HapII 711 CCGG C^CGG
HgaI 421 GACGC GACGC(5/10)
HpaII 711 CCGG C^CGG
MaeI 1123 CTAG C^TAG
MaeII 1279 ACGT A^CGT
MamI 679 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1365 AGATCC [GA]^GATC[CT]
MseI 1028 TTAA T^TAA
MspA1I 749 CAGCTG C[AC]G^C[GT]G
MspI 711 CCGG C^CGG
MvnI 1098 CGCG CG^CG
MwoI 1184 GCTTGTGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 710 CCCGG CC^[GC]GG
PshAI 321 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 749 CAGCTG CAG^CTG
RcaI 659 TCATGA T^CATGA
Sau96I 1396 GGACC G^G[ACGT]CC
SfcI 902 CTACAG C^T[GA][CT]AG
Tru9I 1028 TTAA T^TAA
TseI 619 GCTGC G^C[AT]GC
XcmI 795 CCATGAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1365 AGATCC [GA]^GATC[CT]
XmnI 226 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 736 GTAC GT^AC
847 GTAC
AspHI 913 GAGCTC G[AT]GC[AT]^C
1312 GAGCTC
BalI 366 TGGCCA TGG^CCA
441 TGGCCA
BsaI 1047 GGTCTC GGTCTC(1/5)
1108 GGTCTC
BseRI 113 GAGGAG GAGGAG(10/8)
955 GAGGAG
BsiHKAI 913 GAGCTC G[AT]GC[AT]^C
1312 GAGCTC
DpnII 295 GATC ^GATC
1366 GATC
EcoT14I 688 CCTTGG C^C[AT][AT]GG
1122 CCTAGG
Fnu4HI 518 GCCGC GC^[ACGT]GC
619 GCTGC
HincII 1089 GTCAAC GT[CT]^[GA]AC
1297 GTTGAC
HindII 1089 GTCAAC GT[CT]^[GA]AC
1297 GTTGAC
ItaI 518 GCCGC GC^[ACGT]GC
619 GCTGC
MboI 295 GATC ^GATC
1366 GATC
MluNI 366 TGGCCA TGG^CCA
441 TGGCCA
MscI 366 TGGCCA TGG^CCA
441 TGGCCA
NdeII 295 GATC ^GATC
1366 GATC
PleI 694 GACTC GAGTC(4/5)
1385 GACTC
RsaI 736 GTAC GT^AC
847 GTAC
SacI 913 GAGCTC GAGCT^C
1312 GAGCTC
Sau3AI 295 GATC ^GATC
1366 GATC
SfaNI 531 GCATC GCATC(5/9)
557 GCATC
SmlI 76 CTTGAG C^T[CT][GA]AG
811 CTTGAG
SspI 266 AATATT AAT^ATT
506 AATATT
StyI 688 CCTTGG C^C[AT][AT]GG
1122 CCTAGG
TfiI 780 GAATC G^A[AT]TC
1018 GATTC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BanII BmyI BsaJI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I DdeI EaeI EcoRII
FokI HaeIII HinfI HphI MaeIII
MboII MnlI MslI MvaI NlaIII
NlaIV ScrFI Tsp45I Tsp509I TspRI