Restriction map for
pFN21AE5115
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MboII
MluI MluNI MroI MscI MseI
MslI MspA1I MspI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I TseI Tsp45I Tth111I
Van91I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 290 CGCG CG^CG
AcsI 130 GAATTT [GA]^AATT[CT]
AfaI 72 GTAC GT^AC
AlwI 93 GATCC GGATC(4/5)
ApaI 83 GGGCCC GGGCC^C
ApoI 130 GAATTT [GA]^AATT[CT]
AspHI 308 GAGCTC G[AT]GC[AT]^C
AvaI 212 CTCGAG C^[CT]CG[GA]G
BanI 207 GGTGCC G^G[CT][GA]CC
BfaI 256 CTAG C^TAG
BsaJI 34 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 308 GAGCTC G[AT]GC[AT]^C
BsmAI 259 GTCTC GTCTC(1/5)
BsoBI 212 CTCGAG C^[CT]CG[GA]G
BspMI 64 ACCTGC ACCTGC(4/8)
BssKI 34 CCTGG ^CC[ACGT]GG
BstF5I 244 GGATG GGATG(2/0)
BstNI 34 CCTGG CC^[AT]GG
BstUI 290 CGCG CG^CG
BstYI 92 AGATCC [GA]^GATC[CT]
DdeI 97 CTCAG C^T[ACGT]AG
DraII 82 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 82 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 34 CCTGG ^CC[AT]GG
EcoRV 139 GATATC GAT^ATC
Fnu4HI 291 GCGGC GC^[ACGT]GC
FokI 244 GGATG GGATG(9/13)
HaeIII 84 GGCC GG^CC
HgaI 288 GACGC GACGC(5/10)
ItaI 291 GCGGC GC^[ACGT]GC
MaeI 256 CTAG C^TAG
MfeI 103 CAATTG C^AATTG
MflI 92 AGATCC [GA]^GATC[CT]
MunI 103 CAATTG C^AATTG
MvaI 34 CCTGG CC^[AT]GG
MvnI 290 CGCG CG^CG
PaeR7I 212 CTCGAG C^TCGAG
PleI 215 GAGTC GAGTC(4/5)
RsaI 72 GTAC GT^AC
SacI 308 GAGCTC GAGCT^C
ScrFI 34 CCTGG CC^[ACGT]GG
SfaNI 245 GATGC GCATC(5/9)
TfiI 168 GATTC G^A[AT]TC
TspRI 249 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 212 CTCGAG C^TCGAG
XhoII 92 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 242 GCGG CCGC(-3/-1)
291 GCGG
AluI 188 AGCT AG^CT
309 AGCT
BanII 83 GGGCCC G[GA]GC[CT]^C
308 GAGCTC
BmyI 83 GGGCCC G[AGT]GC[ACT]^C
308 GAGCTC
Bsp1286I 83 GGGCCC G[AGT]GC[ACT]^C
308 GAGCTC
Cfr13I 83 GGGCC G^G[ACGT]CC
84 GGCCC
DpnII 93 GATC ^GATC
253 GATC
HinfI 168 GATTC G^A[ACGT]TC
215 GAGTC
MboI 93 GATC ^GATC
253 GATC
NdeII 93 GATC ^GATC
253 GATC
NlaIV 83 GGGCCC GG[ACGT]^[ACGT]CC
207 GGTGCC
PstI 75 CTGCAG CTGCA^G
313 CTGCAG
Sau3AI 93 GATC ^GATC
253 GATC
Sau96I 83 GGGCC G^G[ACGT]CC
84 GGCCC
SfcI 75 CTGCAG C^T[GA][CT]AG
313 CTGCAG
SmlI 5 CTCAAG C^T[CT][GA]AG
212 CTCGAG
TaqI 137 TCGA T^CGA
213 TCGA
Tsp509I 104 AATT ^AATT
131 AATT
TthHB8I 137 TCGA T^CGA
213 TCGA
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
MnlI MwoI