##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT22I     FbaI        
FokI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvaI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          223  GTCTAC               GT^[AC][GT]AC
AciI           67  CCGC                 CCGC(-3/-1)
AfaI            3  GTAC                 GT^AC
BbvI          327  GCAGC                GCAGC(8/12)
BcgI           43  GCATGGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          217  GTGCCC               G[AGT]GC[ACT]^C
BsaJI         269  CCATGG               C^C[ACGT][ACGT]GG
BsaWI         260  ACCGGA               [AT]^CCGG[AT]
BsiYI         312  CCTCTACATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          312  CCTCTACATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         289  GGGAC                GGGAC(10/14)
Bsp1286I      217  GTGCCC               G[AGT]GC[ACT]^C
CfoI          274  GCGC                 GCG^C
DpnII         306  GATC                 ^GATC
DsaI          269  CCATGG               C^C[GA][CT]GG
EcoT14I       269  CCATGG               C^C[AT][AT]GG
FauI           66  CCCGC                CCCGC(4/6)
Fnu4HI        327  GCAGC                GC^[ACGT]GC
HaeII         273  GGCGCT               [GA]GCGC^[CT]
HapII         261  CCGG                 C^CGG
HhaI          274  GCGC                 GCG^C
HinP1I        274  GCGC                 G^CGC
HinfI         142  GATTC                G^A[ACGT]TC
HpaII         261  CCGG                 C^CGG
ItaI          327  GCAGC                GC^[ACGT]GC
MboI          306  GATC                 ^GATC
MseI           76  TTAA                 T^TAA
MspI          261  CCGG                 C^CGG
MwoI           49  GCTTCGGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          269  CCATGG               C^CATGG
NdeII         306  GATC                 ^GATC
NlaIV         289  GGGACC               GG[ACGT]^[ACGT]CC
RsaI            3  GTAC                 GT^AC
Sau3AI        306  GATC                 ^GATC
SfcI           60  CTATAG               C^T[GA][CT]AG
StyI          269  CCATGG               C^C[AT][AT]GG
TfiI          142  GATTC                G^A[AT]TC
Tru9I          76  TTAA                 T^TAA
TseI          327  GCAGC                G^C[AT]GC
TspRI         149  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          201  CCAGAAAGCTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AluI          185  AGCT                 AG^CT
              207  AGCT                
AvaII         263  GGACC                G^G[AT]CC
              290  GGACC               
Cac8I          65  GCCCGC               GC[ACGT]^[ACGT]GC
              239  GCCTGC              
Cfr13I        263  GGACC                G^G[ACGT]CC
              290  GGACC               
HaeIII         57  GGCC                 GG^CC
              238  GGCC                
Sau96I        263  GGACC                G^G[ACGT]CC
              290  GGACC               
StuI           56  AGGCCT               AGG^CCT
              237  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MnlI        NlaIII