Restriction map for
pFN21AE3905
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I AspEI AspI AvaII
AvrII BalI BamHI BbeI BbrPI
BcgI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KspI MamI MluI
MluNI MroI MscI MslI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 1203 CCGC CCGC(-3/-1)
AflIII 1237 ACGTGT A^C[GA][CT]GT
AseI 616 ATTAAT AT^TAAT
AsnI 616 ATTAAT AT^TAAT
AspHI 1111 GAGCTC G[AT]GC[AT]^C
AvaI 187 CCCGAG C^[CT]CG[GA]G
AviII 1017 TGCGCA TGC^GCA
BanII 1111 GAGCTC G[GA]GC[CT]^C
BbvCI 909 CCTCAGC CCTCAGC(-5/-2)
BciVI 50 GTATCC GTATCC(6/5)
BclI 890 TGATCA T^GATCA
BglII 1190 AGATCT A^GATCT
BsaAI 856 TACGTA [CT]AC^GT[GA]
BsiHKAI 1111 GAGCTC G[AT]GC[AT]^C
BsoBI 187 CCCGAG C^[CT]CG[GA]G
Bsp1407I 777 TGTACA T^GTACA
BsrDI 1047 CATTGC GCAATG(2/0)
BsrFI 34 ACCGGC [GA]^CCGG[CT]
BsrGI 777 TGTACA T^GTACA
Cac8I 823 GCTCGC GC[ACGT]^[ACGT]GC
Cfr10I 34 ACCGGC [GA]^CCGG[CT]
Cfr13I 882 GGGCC G^G[ACGT]CC
EarI 206 GAAGAG CTCTTC(1/4)
EcoRV 991 GATATC GAT^ATC
FauI 1202 CCCGC CCCGC(4/6)
FbaI 890 TGATCA T^GATCA
FspI 1017 TGCGCA TGC^GCA
HapII 35 CCGG C^CGG
HincII 220 GTTGAC GT[CT]^[GA]AC
HindII 220 GTTGAC GT[CT]^[GA]AC
HpaII 35 CCGG C^CGG
Ksp632I 206 GAAGAG CTCTTC(1/4)
MfeI 1124 CAATTG C^AATTG
MspA1I 256 CAGCTG C[AC]G^C[GT]G
MspI 35 CCGG C^CGG
MunI 1124 CAATTG C^AATTG
MwoI 905 GCATCCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 679 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 190 GAGTC GAGTC(4/5)
PshBI 616 ATTAAT AT^TAAT
PvuII 256 CAGCTG CAG^CTG
SacI 1111 GAGCTC GAGCT^C
Sau96I 882 GGGCC G^G[ACGT]CC
SfcI 1052 CTATAG C^T[GA][CT]AG
SnaBI 856 TACGTA TAC^GTA
SspBI 777 TGTACA T^GTACA
Van91I 679 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 233 CCAAGTTCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 739 GGTACC G^GTACC
1161 GGTACC
Asp718I 739 GGTACC G^GTACC
1161 GGTACC
BbsI 492 GAAGAC GAAGAC(2/6)
1330 GAAGAC
BfaI 386 CTAG C^TAG
430 CTAG
BmyI 30 GGGCAC G[AGT]GC[ACT]^C
1111 GAGCTC
BpuAI 492 GAAGAC GAAGAC(2/6)
1330 GAAGAC
BseRI 7 GAGGAG GAGGAG(10/8)
119 GAGGAG
BsiYI 109 CCTTACTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
679 CCACTCTATGG
BslI 109 CCTTACTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
679 CCACTCTATGG
BsmAI 209 GAGAC GTCTC(1/5)
1231 GAGAC
Bsp1286I 30 GGGCAC G[AGT]GC[ACT]^C
1111 GAGCTC
BsrI 212 ACTGG ACTGG(1/-1)
553 ACTGG
BstF5I 906 CATCC GGATG(2/0)
1268 GGATG
BstYI 459 AGATCC [GA]^GATC[CT]
1190 AGATCT
CfoI 1018 GCGC GCG^C
1305 GCGC
DraI 166 TTTAAA TTT^AAA
304 TTTAAA
FokI 906 CATCC GGATG(9/13)
1268 GGATG
HhaI 1018 GCGC GCG^C
1305 GCGC
HinP1I 1018 GCGC G^CGC
1305 GCGC
HphI 888 GGTGA GGTGA(8/7)
181 TCACC
KpnI 739 GGTACC GGTAC^C
1161 GGTACC
MaeI 386 CTAG C^TAG
430 CTAG
MflI 459 AGATCC [GA]^GATC[CT]
1190 AGATCT
NdeI 94 CATATG CA^TATG
1208 CATATG
NlaIII 559 CATG CATG^
1005 CATG
TfiI 228 GATTC G^A[AT]TC
640 GAATC
XhoII 459 AGATCC [GA]^GATC[CT]
1190 AGATCT
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BanI BbvI BsaJI BssKI BstNI
DdeI DpnII EcoRII EcoT14I Fnu4HI
HaeIII HinfI ItaI MaeII MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIV RsaI Sau3AI ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I