Restriction map for
pFN21AE1356
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# NO CUT ENZYMES ( 201 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FokI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AciI 53 CCGC CCGC(-3/-1)
AvaI 72 CCCGAG C^[CT]CG[GA]G
BanII 176 GGGCTC G[GA]GC[CT]^C
BclI 153 TGATCA T^GATCA
BglII 65 AGATCT A^GATCT
BlpI 178 GCTCAGC GC^T[ACGT]AGC
BmyI 176 GGGCTC G[AGT]GC[ACT]^C
BpmI 117 CTCCAG CTGGAG(16/14)
Bpu1102I 178 GCTCAGC GC^T[ACGT]AGC
BseRI 100 CTCCTC GAGGAG(10/8)
BsoBI 72 CCCGAG C^[CT]CG[GA]G
Bsp1286I 176 GGGCTC G[AGT]GC[ACT]^C
BssKI 24 CCTGG ^CC[ACGT]GG
BstNI 24 CCTGG CC^[AT]GG
BstYI 65 AGATCT [GA]^GATC[CT]
CelII 178 GCTCAGC GC^T[ACGT]AGC
DdeI 179 CTCAG C^T[ACGT]AG
DsaI 134 CCATGG C^C[GA][CT]GG
EcoRII 24 CCTGG ^CC[AT]GG
EcoT14I 134 CCATGG C^C[AT][AT]GG
FbaI 153 TGATCA T^GATCA
MboII 186 GAAGA GAAGA(8/7)
MflI 65 AGATCT [GA]^GATC[CT]
MseI 233 TTAA T^TAA
MspA1I 181 CAGCTG C[AC]G^C[GT]G
MvaI 24 CCTGG CC^[AT]GG
MwoI 183 GCTGAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 134 CCATGG C^CATGG
PvuII 181 CAGCTG CAG^CTG
ScrFI 24 CCTGG CC^[ACGT]GG
SfaNI 227 GCATC GCATC(5/9)
StyI 134 CCATGG C^C[AT][AT]GG
TaqI 92 TCGA T^CGA
Tru9I 233 TTAA T^TAA
TthHB8I 92 TCGA T^CGA
XhoII 65 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BsaJI 23 CCCTGG C^C[ACGT][ACGT]GG
134 CCATGG
BsiYI 18 CCTCACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
99 CCTCCTCATGG
BslI 18 CCTCACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
99 CCTCCTCATGG
BsrI 33 ACTGG ACTGG(1/-1)
119 CCAGT
DpnII 66 GATC ^GATC
154 GATC
HphI 14 TCACC GGTGA(8/7)
20 TCACC
MaeIII 13 GTCAC ^GT[ACGT]AC
28 GTCAC
MboI 66 GATC ^GATC
154 GATC
NdeII 66 GATC ^GATC
154 GATC
PstI 45 CTGCAG CTGCA^G
59 CTGCAG
Sau3AI 66 GATC ^GATC
154 GATC
SfcI 45 CTGCAG C^T[GA][CT]AG
59 CTGCAG
Tsp45I 13 GTCAC ^GT[GC]AC
28 GTCAC
TspRI 32 CACTG CA[GC]TG[ACGT][ACGT]^
263 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI BbvI Fnu4HI ItaI MnlI
NlaIII TseI