Restriction map for
pFN21AE1368
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BbeI BbvCI
BcgI BciVI BfrI BglII BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FspI HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MseI MunI NarI NdeI
NheI NotI NruI NspI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SfoI SfuI SmlI
SnaBI SpeI SphI SspI StuI
SwaI TaqI TfiI Tru9I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 1096 AAATTC [GA]^AATT[CT]
AocI 924 CCTCAGG CC^T[ACGT]AGG
ApoI 1096 AAATTC [GA]^AATT[CT]
AspI 268 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1303 CCTAGG C^CTAGG
BalI 1496 TGGCCA TGG^CCA
BamHI 779 GGATCC G^GATCC
BbrPI 123 CACGTG CAC^GTG
BbsI 1453 GTCTTC GAAGAC(2/6)
BclI 656 TGATCA T^GATCA
BfaI 1304 CTAG C^TAG
BglI 1483 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1303 CCTAGG C^CTAGG
BpuAI 1453 GTCTTC GAAGAC(2/6)
BsaAI 123 CACGTG [CT]AC^GT[GA]
BsiEI 1353 CGACCG CG[GA][CT]^CG
Bsp1407I 1130 TGTACA T^GTACA
BsrDI 1134 CATTGC GCAATG(2/0)
BsrGI 1130 TGTACA T^GTACA
BstEII 363 GGTGACC G^GT[ACGT]ACC
BstPI 363 GGTGACC G^GT[ACGT]ACC
BstXI 598 CCAGGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 924 CCTCAGG CC^T[ACGT]AGG
DraIII 1335 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1354 GACCGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 924 CCTCAGG CC^T[ACGT]AGG
EcoO65I 363 GGTGACC G^GT[ACGT]ACC
EcoT22I 1346 ATGCAT ATGCA^T
FbaI 656 TGATCA T^GATCA
FseI 969 GGCCGGCC GGCCGG^CC
KspI 62 CCGCGG CCGC^GG
MaeI 1304 CTAG C^TAG
MaeII 124 ACGT A^CGT
MluNI 1496 TGGCCA TGG^CCA
MscI 1496 TGGCCA TGG^CCA
MslI 1998 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 474 CCATGG C^CATGG
NsiI 1346 ATGCAT ATGCA^T
PflFI 268 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 123 CACGTG CAC^GTG
PmlI 123 CACGTG CAC^GTG
PshAI 646 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 62 CCGCGG CCGC^GG
SbfI 1855 CCTGCAGG CCTGCA^GG
SexAI 269 ACCAGGT A^CC[AT]GGT
SgrAI 23 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1855 CCTGCAGG CCTGCA^GG
SspBI 1130 TGTACA T^GTACA
Tsp509I 1097 AATT ^AATT
Tth111I 268 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 90 GTCTAC GT^[AC][GT]AC
460 GTCTAC
AcyI 21 GACGCC G[GA]^CG[CT]C
46 GACGCC
AfaI 312 GTAC GT^AC
1131 GTAC
ApaI 1184 GGGCCC GGGCC^C
1650 GGGCCC
BlpI 672 GCTCAGC GC^T[ACGT]AGC
1800 GCTGAGC
Bpu1102I 672 GCTCAGC GC^T[ACGT]AGC
1800 GCTGAGC
BsaHI 21 GACGCC G[GA]^CG[CT]C
46 GACGCC
BsaI 1110 GGTCTC GGTCTC(1/5)
1989 GGTCTC
BstAPI 198 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1334 GCACCGCGTGC
BstYI 779 GGATCC [GA]^GATC[CT]
1150 AGATCC
CelII 672 GCTCAGC GC^T[ACGT]AGC
1800 GCTGAGC
EarI 76 CTCTTC CTCTTC(1/4)
249 GAAGAG
HaeII 1020 GGCGCT [GA]GCGC^[CT]
1480 AGCGCC
Hin1I 21 GACGCC G[GA]^CG[CT]C
46 GACGCC
Ksp632I 76 CTCTTC CTCTTC(1/4)
249 GAAGAG
MflI 779 GGATCC [GA]^GATC[CT]
1150 AGATCC
PvuII 628 CAGCTG CAG^CTG
660 CAGCTG
RsaI 312 GTAC GT^AC
1131 GTAC
SapI 249 GAAGAGC GCTCTTC(1/4)
75 GCTCTTC
XcmI 1316 CCATGCCCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1484 CCATCAGGGCAGTGG
XhoII 779 GGATCC [GA]^GATC[CT]
1150 AGATCC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TseI Tsp45I
TspRI Van91I XmaCI XmaI