Restriction map for
pFN21AE1431
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# NO CUT ENZYMES ( 102 enzymes) #
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AccII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspEI BsrDI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII CfoI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoT22I FseI FspI
HaeII HhaI HinP1I HpaI KasI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1111 GACGTC GACGT^C
AccI 1933 GTCTAC GT^[AC][GT]AC
AcyI 1111 GACGTC G[GA]^CG[CT]C
AseI 1681 ATTAAT AT^TAAT
AsnI 1681 ATTAAT AT^TAAT
AspHI 246 GAGCAC G[AT]GC[AT]^C
BamHI 103 GGATCC G^GATCC
BanII 414 GGGCTC G[GA]GC[CT]^C
BbvCI 739 GCTGAGG CCTCAGC(-5/-2)
BclI 1736 TGATCA T^GATCA
BglI 1072 GCCCTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1111 GACGTC G[GA]^CG[CT]C
BsiHKAI 246 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1085 TGTACA T^GTACA
BspDI 1405 ATCGAT AT^CGAT
BspHI 1157 TCATGA T^CATGA
BspLU11I 1350 ACATGT A^CATGT
BsrFI 446 GCCGGT [GA]^CCGG[CT]
BsrGI 1085 TGTACA T^GTACA
BssSI 1522 CACGAG CACGAG(-5/-1)
BstEII 2025 GGTCACC G^GT[ACGT]ACC
BstPI 2025 GGTCACC G^GT[ACGT]ACC
BstXI 435 CCAAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 446 GCCGGT [GA]^CCGG[CT]
ClaI 1405 ATCGAT AT^CGAT
EarI 1858 GAAGAG CTCTTC(1/4)
EcoNI 1117 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2025 GGTCACC G^GT[ACGT]ACC
EcoRI 1703 GAATTC G^AATTC
EcoRV 1879 GATATC GAT^ATC
FbaI 1736 TGATCA T^GATCA
HgaI 557 GCGTC GACGC(5/10)
Hin1I 1111 GACGTC G[GA]^CG[CT]C
HincII 880 GTCAAC GT[CT]^[GA]AC
HindII 880 GTCAAC GT[CT]^[GA]AC
HindIII 695 AAGCTT A^AGCTT
Ksp632I 1858 GAAGAG CTCTTC(1/4)
NdeI 938 CATATG CA^TATG
NspI 1350 ACATGT [GA]CATG^[CT]
PaeR7I 1811 CTCGAG C^TCGAG
PshBI 1681 ATTAAT AT^TAAT
RcaI 1157 TCATGA T^CATGA
SapI 1858 GAAGAGC GCTCTTC(1/4)
ScaI 1553 AGTACT AGT^ACT
SexAI 815 ACCTGGT A^CC[AT]GGT
SmaI 501 CCCGGG CCC^GGG
SspBI 1085 TGTACA T^GTACA
TfiI 2092 GATTC G^A[AT]TC
XhoI 1811 CTCGAG C^TCGAG
XmaCI 501 CCCGGG C^CCGGG
XmaI 501 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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Acc65I 1459 GGTACC G^GTACC
2199 GGTACC
AlwNI 166 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1417 CAGTCTCTG
Asp718I 1459 GGTACC G^GTACC
2199 GGTACC
BalI 1102 TGGCCA TGG^CCA
1764 TGGCCA
BanI 1459 GGTACC G^G[CT][GA]CC
2199 GGTACC
BsaAI 178 TACGTG [CT]AC^GT[GA]
218 CACGTA
BsaI 718 GAGACC GGTCTC(1/5)
350 GGTCTC
BsrBI 482 GAGCGG CCGCTC(-3/-3)
633 GAGCGG
DsaI 79 CCATGG C^C[GA][CT]GG
1480 CCATGG
KpnI 1459 GGTACC GGTAC^C
2199 GGTACC
MluNI 1102 TGGCCA TGG^CCA
1764 TGGCCA
MscI 1102 TGGCCA TGG^CCA
1764 TGGCCA
MseI 886 TTAA T^TAA
1682 TTAA
MslI 388 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1152 CATCCTCATG
NcoI 79 CCATGG C^CATGG
1480 CCATGG
PstI 225 CTGCAG CTGCA^G
570 CTGCAG
PvuII 1962 CAGCTG CAG^CTG
2151 CAGCTG
SfcI 225 CTGCAG C^T[GA][CT]AG
570 CTGCAG
Tru9I 886 TTAA T^TAA
1682 TTAA
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspI AvaI AvaII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII