##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DpnII       DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tsp45I      Tsp509I     
TspRI       Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AciI          264  GCGG                 CCGC(-3/-1)
AcyI           94  GACGCC               G[GA]^CG[CT]C
AspI          289  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          312  GAAGAC               GAAGAC(2/6)
BpuAI         312  GAAGAC               GAAGAC(2/6)
BsaHI          94  GACGCC               G[GA]^CG[CT]C
BsaJI          41  CCAAGG               C^C[ACGT][ACGT]GG
BsiYI         100  CCTGCTAACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          100  CCTGCTAACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          92  GAGAC                GTCTC(1/5)
BsmBI          92  GAGACG               CGTCTC(1/5)
BsmI          111  GAATGC               GAATGC(1/-1)
DdeI          245  CTGAG                C^T[ACGT]AG
EcoT14I        41  CCAAGG               C^C[AT][AT]GG
FauI          264  GCGGG                CCCGC(4/6)
Hin1I          94  GACGCC               G[GA]^CG[CT]C
HinfI         247  GAGTC                G^A[ACGT]TC
MseI           49  TTAA                 T^TAA
MspA1I        269  CAGCTG               C[AC]G^C[GT]G
MwoI          244  GCTGAGTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI         289  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          247  GAGTC                GAGTC(4/5)
PvuII         269  CAGCTG               CAG^CTG
StyI           41  CCAAGG               C^C[AT][AT]GG
TaqI          296  TCGA                 T^CGA
Tru9I          49  TTAA                 T^TAA
Tth111I       289  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       296  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AccI           16  GTAGAC               GT^[AC][GT]AC
              154  GTAGAC              
BbvI           10  GCAGC                GCAGC(8/12)
              268  GCAGC               
BseRI         187  GAGGAG               GAGGAG(10/8)
              190  GAGGAG              
Cac8I         260  GCAAGC               GC[ACGT]^[ACGT]GC
              264  GCGGGC              
Fnu4HI         10  GCAGC                GC^[ACGT]GC
              268  GCAGC               
HgaI            7  GACGC                GACGC(5/10)
               94  GACGC               
ItaI           10  GCAGC                GC^[ACGT]GC
              268  GCAGC               
TseI           10  GCAGC                G^C[AT]GC
              268  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        BstF5I      EarI        FokI        HphI        
Ksp632I     MboII       MnlI