Restriction map for
pFN21AE3606
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI BstBI
BstEII BstPI CelII ClaI DraI
DraIII Eam1105I EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeII MamI MfeI
MluI MroI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI SacI SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 640 GTATAC GT^[AC][GT]AC
AfaI 629 GTAC GT^AC
AocI 427 CCTCAGG CC^T[ACGT]AGG
Aor51HI 365 AGCGCT AGC^GCT
BamHI 318 GGATCC G^GATCC
BanI 90 GGTGCC G^G[CT][GA]CC
BsaI 655 GAGACC GGTCTC(1/5)
BsgI 1321 CTGCAC GTGCAG(16/14)
BspMI 87 GCAGGT ACCTGC(4/8)
Bst1107I 640 GTATAC GTA^TAC
BstAPI 864 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 587 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 640 GTATAC GTA^TAC
Bsu36I 427 CCTCAGG CC^T[ACGT]AGG
CpoI 104 CGGTCCG CG^G[AT]CCG
DrdI 544 GACTGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 122 CGGCCG C^GGCCG
EclXI 122 CGGCCG C^GGCCG
Eco47III 365 AGCGCT AGC^GCT
Eco52I 122 CGGCCG C^GGCCG
Eco81I 427 CCTCAGG CC^T[ACGT]AGG
EcoNI 555 CCTTAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1161 GATATC GAT^ATC
HphI 1002 GGTGA GGTGA(8/7)
KspI 263 CCGCGG CCGC^GG
MaeIII 542 GTGAC ^GT[ACGT]AC
NaeI 124 GCCGGC GCC^GGC
NgoMIV 124 GCCGGC G^CCGGC
PstI 508 CTGCAG CTGCA^G
RsaI 629 GTAC GT^AC
RsrII 104 CGGTCCG CG^G[AT]CCG
SacII 263 CCGCGG CCGC^GG
SapI 466 GAAGAGC GCTCTTC(1/4)
SpeI 18 ACTAGT A^CTAGT
StuI 1295 AGGCCT AGG^CCT
Tsp45I 542 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BalI 356 TGGCCA TGG^CCA
647 TGGCCA
BbsI 448 GTCTTC GAAGAC(2/6)
1377 GTCTTC
BglII 1143 AGATCT A^GATCT
1277 AGATCT
BpuAI 448 GTCTTC GAAGAC(2/6)
1377 GTCTTC
BsiEI 122 CGGCCG CG[GA][CT]^CG
162 CGACCG
BsmBI 7 GAGACG CGTCTC(1/5)
1354 GAGACG
BsrDI 441 CATTGC GCAATG(2/0)
929 GCAATG
BsrFI 124 GCCGGC [GA]^CCGG[CT]
860 ACCGGC
BstF5I 909 CATCC GGATG(2/0)
1063 CATCC
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
860 ACCGGC
DsaI 227 CCGTGG C^C[GA][CT]GG
263 CCGCGG
FauI 33 GCGGG CCCGC(4/6)
1269 GCGGG
FokI 909 CATCC GGATG(9/13)
1063 CATCC
HaeII 73 GGCGCT [GA]GCGC^[CT]
365 AGCGCT
MluNI 356 TGGCCA TGG^CCA
647 TGGCCA
MscI 356 TGGCCA TGG^CCA
647 TGGCCA
NlaIII 1266 CATG CATG^
1430 CATG
PpuMI 422 AGGACCC [GA]G^G[AT]CC[CT]
1196 AGGACCT
SfcI 508 CTGCAG C^T[GA][CT]AG
666 CTATAG
SfiI 252 GGCCGCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
348 GGCCGAGCTGGCC
SmaI 94 CCCGGG CCC^GGG
194 CCCGGG
XmaCI 94 CCCGGG C^CCGGG
194 CCCGGG
XmaI 94 CCCGGG C^CCGGG
194 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI Asp700I AvaI AvaII BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV PflMI PleI Sau3AI
Sau96I ScrFI SfaNI TaqI Tru9I
TseI TspRI TthHB8I Van91I XhoII
XmnI