Restriction map for
pFN21AE1544
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cac8I CelII
CfoI ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI HinP1I KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 675 GACGCC G[GA]^CG[CT]C
AflII 961 CTTAAG C^TTAAG
AflIII 732 ACGTGT A^C[GA][CT]GT
AlwI 957 GGATC GGATC(4/5)
AlwNI 153 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 258 CCTCAGG CC^T[ACGT]AGG
Asp700I 310 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 372 TGATCA T^GATCA
BfaI 727 CTAG C^TAG
BfrI 961 CTTAAG C^TTAAG
BsaAI 972 TACGTG [CT]AC^GT[GA]
BsaHI 675 GACGCC G[GA]^CG[CT]C
BsaWI 436 TCCGGT [AT]^CCGG[AT]
BseRI 256 CTCCTC GAGGAG(10/8)
BsmI 214 GAATGC GAATGC(1/-1)
BspMI 797 GCAGGT ACCTGC(4/8)
BsrFI 67 ACCGGC [GA]^CCGG[CT]
BstYI 957 GGATCT [GA]^GATC[CT]
Bsu36I 258 CCTCAGG CC^T[ACGT]AGG
Cfr10I 67 ACCGGC [GA]^CCGG[CT]
DrdI 514 GACCTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 258 CCTCAGG CC^T[ACGT]AGG
EcoNI 391 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 586 CCTTGG C^C[AT][AT]GG
FbaI 372 TGATCA T^GATCA
HgaI 675 GACGC GACGC(5/10)
Hin1I 675 GACGCC G[GA]^CG[CT]C
HincII 124 GTTAAC GT[CT]^[GA]AC
HindII 124 GTTAAC GT[CT]^[GA]AC
HindIII 949 AAGCTT A^AGCTT
HpaI 124 GTTAAC GTT^AAC
HphI 944 TCACC GGTGA(8/7)
MaeI 727 CTAG C^TAG
MflI 957 GGATCT [GA]^GATC[CT]
MslI 714 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 1223 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 843 GAGTC GAGTC(4/5)
PpuMI 799 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 828 GAGCTC GAGCT^C
StyI 586 CCTTGG C^C[AT][AT]GG
TaqI 1144 TCGA T^CGA
TthHB8I 1144 TCGA T^CGA
Van91I 1223 CCATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 957 GGATCT [GA]^GATC[CT]
XmnI 310 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 550 GCGG CCGC(-3/-1)
1329 GCGG
AcsI 91 GAATTT [GA]^AATT[CT]
664 GAATTT
AfaI 858 GTAC GT^AC
1281 GTAC
ApaI 582 GGGCCC GGGCC^C
1274 GGGCCC
ApoI 91 GAATTT [GA]^AATT[CT]
664 GAATTT
AspHI 442 GTGCTC G[AT]GC[AT]^C
828 GAGCTC
AspI 331 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
937 GACATCGTC
BalI 536 TGGCCA TGG^CCA
1220 TGGCCA
BsiHKAI 442 GTGCTC G[AT]GC[AT]^C
828 GAGCTC
BsmFI 1046 GGGAC GGGAC(10/14)
801 GTCCC
BstXI 713 CCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
947 CCAAGCTTCTGG
EaeI 536 TGGCCA [CT]^GGCC[GA]
1220 TGGCCA
MaeII 732 ACGT A^CGT
973 ACGT
MluNI 536 TGGCCA TGG^CCA
1220 TGGCCA
MscI 536 TGGCCA TGG^CCA
1220 TGGCCA
MspA1I 577 CAGCTG C[AC]G^C[GT]G
1024 CAGCTG
NlaIII 720 CATG CATG^
1224 CATG
PflFI 331 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
937 GACATCGTC
PvuII 577 CAGCTG CAG^CTG
1024 CAGCTG
RsaI 858 GTAC GT^AC
1281 GTAC
Tsp45I 164 GTCAC ^GT[GC]AC
943 GTCAC
Tth111I 331 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
937 GACATCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AluI AvaII BanII BbsI BbvI
BmyI BpmI BpuAI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI SfaNI SmlI TfiI
Tru9I TseI Tsp509I TspRI