Restriction map for
pFN21AB7117
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# NO CUT ENZYMES ( 153 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmAI BsmBI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MflI
MluI MroI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tru9I Tsp45I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 505 GACGTC GACGT^C
AccI 508 GTCTAC GT^[AC][GT]AC
AcyI 505 GACGTC G[GA]^CG[CT]C
AvaI 696 CTCGGG C^[CT]CG[GA]G
BalI 272 TGGCCA TGG^CCA
BanI 802 GGCACC G^G[CT][GA]CC
BbrPI 280 CACGTG CAC^GTG
BsaAI 280 CACGTG [CT]AC^GT[GA]
BsaHI 505 GACGTC G[GA]^CG[CT]C
BsiEI 831 CGACCG CG[GA][CT]^CG
BsmI 45 GCATTC GAATGC(1/-1)
BsoBI 696 CTCGGG C^[CT]CG[GA]G
Bsp1407I 17 TGTACA T^GTACA
BsrBI 669 CCGCTC CCGCTC(-3/-3)
BsrFI 41 ACCGGC [GA]^CCGG[CT]
BsrGI 17 TGTACA T^GTACA
BsrI 5 ACTGG ACTGG(1/-1)
BssSI 268 CTCGTG CACGAG(-5/-1)
Cfr10I 41 ACCGGC [GA]^CCGG[CT]
DpnII 741 GATC ^GATC
DraII 335 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 360 GAAGAG CTCTTC(1/4)
EcoNI 348 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 335 GGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 665 AGCGCC [GA]GCGC^[CT]
Hin1I 505 GACGTC G[GA]^CG[CT]C
HinfI 95 GAATC G^A[ACGT]TC
Ksp632I 360 GAAGAG CTCTTC(1/4)
MboI 741 GATC ^GATC
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MspA1I 834 CCGCTG C[AC]G^C[GT]G
NdeII 741 GATC ^GATC
NheI 582 GCTAGC G^CTAGC
PmaCI 280 CACGTG CAC^GTG
PmlI 280 CACGTG CAC^GTG
PpuMI 335 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 399 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 741 GATC ^GATC
SmlI 322 CTTGAG C^T[CT][GA]AG
SspBI 17 TGTACA T^GTACA
TfiI 95 GAATC G^A[AT]TC
Tsp509I 200 AATT ^AATT
XcmI 482 CCATGGTGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 18 GTAC GT^AC
630 GTAC
BanII 659 GAGCCC G[GA]GC[CT]^C
693 GGGCTC
BcnI 642 CCGGG CC^[GC]GG
806 CCGGG
BfaI 247 CTAG C^TAG
583 CTAG
BpmI 166 CTCCAG CTGGAG(16/14)
349 CTGGAG
BseRI 265 CTCCTC GAGGAG(10/8)
352 GAGGAG
BstXI 275 CCATGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
521 CCAACACAGTGG
CfoI 666 GCGC GCG^C
789 GCGC
DsaI 330 CCATGG C^C[GA][CT]GG
482 CCATGG
EcoT14I 330 CCATGG C^C[AT][AT]GG
482 CCATGG
HhaI 666 GCGC GCG^C
789 GCGC
HinP1I 666 GCGC G^CGC
789 GCGC
MaeI 247 CTAG C^TAG
583 CTAG
MaeII 281 ACGT A^CGT
506 ACGT
NciI 642 CCGGG CC^[GC]GG
806 CCGGG
NcoI 330 CCATGG C^CATGG
482 CCATGG
PstI 116 CTGCAG CTGCA^G
235 CTGCAG
RsaI 18 GTAC GT^AC
630 GTAC
SfaNI 596 GCATC GCATC(5/9)
602 GCATC
SfcI 116 CTGCAG C^T[GA][CT]AG
235 CTGCAG
StyI 330 CCATGG C^C[AT][AT]GG
482 CCATGG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AvaII BbsI BbvI
BmyI BpuAI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MboII MnlI
MslI MspI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TseI TspRI