Restriction map for
pFN21AE3619
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRII EcoRV EcoT22I FseI FspI
HapII Hin1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeII MaeIII MamI MfeI
MflI MluI MroI MseI MspI
MunI MvaI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tsp45I Tsp509I TthHB8I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 154 GTAC GT^AC
AocI 46 CCTCAGG CC^T[ACGT]AGG
Aor51HI 476 AGCGCT AGC^GCT
AspHI 341 GAGCAC G[AT]GC[AT]^C
AspI 85 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 570 CCTAGG C^CTAGG
BalI 251 TGGCCA TGG^CCA
BanII 496 GAGCCC G[GA]GC[CT]^C
BbvI 448 GCAGC GCAGC(8/12)
BclI 74 TGATCA T^GATCA
BlnI 570 CCTAGG C^CTAGG
BseRI 487 GAGGAG GAGGAG(10/8)
BsiHKAI 341 GAGCAC G[AT]GC[AT]^C
BsmFI 321 GGGAC GGGAC(10/14)
BsrI 501 CCAGT ACTGG(1/-1)
Bsu36I 46 CCTCAGG CC^T[ACGT]AGG
DpnII 75 GATC ^GATC
DraII 321 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 251 TGGCCA [CT]^GGCC[GA]
EarI 493 GAAGAG CTCTTC(1/4)
Eco47III 476 AGCGCT AGC^GCT
Eco81I 46 CCTCAGG CC^T[ACGT]AGG
EcoO109I 321 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 74 TGATCA T^GATCA
Fnu4HI 448 GCAGC GC^[ACGT]GC
HaeII 476 AGCGCT [GA]GCGC^[CT]
ItaI 448 GCAGC GC^[ACGT]GC
Ksp632I 493 GAAGAG CTCTTC(1/4)
MboI 75 GATC ^GATC
MluNI 251 TGGCCA TGG^CCA
MscI 251 TGGCCA TGG^CCA
MspA1I 255 CAGCGG C[AC]G^C[GT]G
NcoI 408 CCATGG C^CATGG
NdeII 75 GATC ^GATC
PflFI 85 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 418 GACTC GAGTC(4/5)
PpuMI 321 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 154 GTAC GT^AC
SapI 493 GAAGAGC GCTCTTC(1/4)
Sau3AI 75 GATC ^GATC
SfaNI 292 GATGC GCATC(5/9)
SfcI 525 CTATAG C^T[GA][CT]AG
StuI 567 AGGCCT AGG^CCT
TseI 448 GCAGC G^C[AT]GC
Tth111I 85 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 396 CCGC CCGC(-3/-1)
257 GCGG
AlwNI 7 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
232 CAGAGGCTG
AvaII 322 GGACC G^G[AT]CC
574 GGACC
BfaI 67 CTAG C^TAG
571 CTAG
BmyI 341 GAGCAC G[AGT]GC[ACT]^C
496 GAGCCC
Bsp1286I 341 GAGCAC G[AGT]GC[ACT]^C
496 GAGCCC
CfoI 477 GCGC GCG^C
507 GCGC
DdeI 47 CTCAG C^T[ACGT]AG
133 CTAAG
DsaI 369 CCGTGG C^C[GA][CT]GG
408 CCATGG
EcoT14I 408 CCATGG C^C[AT][AT]GG
570 CCTAGG
FauI 395 CCCGC CCCGC(4/6)
257 GCGGG
HgaI 85 GACGC GACGC(5/10)
312 GACGC
HhaI 477 GCGC GCG^C
507 GCGC
HinP1I 477 GCGC G^CGC
507 GCGC
MaeI 67 CTAG C^TAG
571 CTAG
MslI 286 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
327 CACCAGAGTG
StyI 408 CCATGG C^C[AT][AT]GG
570 CCTAGG
XcmI 501 CCAGTTGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
546 CCACTATGTAGCTGG
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
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AluI BpmI BsaJI BsiYI BslI
BstF5I Cac8I Cfr13I FokI HaeIII
HinfI MboII MnlI MwoI NlaIII
NlaIV Sau96I TfiI TspRI