Restriction map for
pFN21AE4522
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AocI Aor51HI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiWI BsmBI BsoBI BspEI BspHI
BspLU11I BspMI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraI DraIII EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI Hin1I
KasI KpnI Ksp632I MluI MluNI
MroI MscI NarI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 493 GTAGAC GT^[AC][GT]AC
AgeI 1141 ACCGGT A^CCGGT
AhdI 342 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 617 GTGCAC G^TGCAC
AlwNI 920 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 363 GGGCCC GGGCC^C
ApaLI 617 GTGCAC G^TGCAC
AspEI 342 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 724 GGTGCC G^G[CT][GA]CC
BanII 363 GGGCCC G[GA]GC[CT]^C
BbsI 1501 GTCTTC GAAGAC(2/6)
BbvCI 1303 GCTGAGG CCTCAGC(-5/-2)
BcgI 56 CGAGCGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 295 GTATCC GTATCC(6/5)
BglII 656 AGATCT A^GATCT
BpuAI 1501 GTCTTC GAAGAC(2/6)
BsaBI 868 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1141 ACCGGT [AT]^CCGG[AT]
BsgI 1667 CTGCAC GTGCAG(16/14)
BsmI 956 GCATTC GAATGC(1/-1)
Bsp1407I 1547 TGTACA T^GTACA
BspDI 1552 ATCGAT AT^CGAT
BsrGI 1547 TGTACA T^GTACA
ClaI 1552 ATCGAT AT^CGAT
EagI 186 CGGCCG C^GGCCG
Eam1105I 342 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 186 CGGCCG C^GGCCG
Eco52I 186 CGGCCG C^GGCCG
EcoT14I 498 CCTTGG C^C[AT][AT]GG
EcoT22I 673 ATGCAT ATGCA^T
HaeII 94 AGCGCC [GA]GCGC^[CT]
HincII 745 GTTAAC GT[CT]^[GA]AC
HindII 745 GTTAAC GT[CT]^[GA]AC
HindIII 578 AAGCTT A^AGCTT
HpaI 745 GTTAAC GTT^AAC
KspI 984 CCGCGG CCGC^GG
MaeII 1571 ACGT A^CGT
MamI 868 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 458 CAATTG C^AATTG
MunI 458 CAATTG C^AATTG
NaeI 144 GCCGGC GCC^GGC
NgoMIV 144 GCCGGC G^CCGGC
NsiI 673 ATGCAT ATGCA^T
NspI 1001 GCATGC [GA]CATG^[CT]
PflMI 1435 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1141 ACCGGT A^CCGGT
PleI 922 GACTC GAGTC(4/5)
PshAI 975 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1645 CTGCAG CTGCA^G
SacII 984 CCGCGG CCGC^GG
SfcI 1645 CTGCAG C^T[GA][CT]AG
SphI 1001 GCATGC GCATG^C
SspBI 1547 TGTACA T^GTACA
SspI 394 AATATT AAT^ATT
StyI 498 CCTTGG C^C[AT][AT]GG
Van91I 1435 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 164 CCACGCTGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 33 GTAC GT^AC
1548 GTAC
AspHI 617 GTGCAC G[AT]GC[AT]^C
1016 GTGCTC
BfaI 1034 CTAG C^TAG
1204 CTAG
BpmI 416 CTCCAG CTGGAG(16/14)
760 CTGGAG
BsaI 820 GAGACC GGTCTC(1/5)
1060 GAGACC
BseRI 1126 GAGGAG GAGGAG(10/8)
1291 GAGGAG
BsiEI 186 CGGCCG CG[GA][CT]^CG
249 CGGTCG
BsiHKAI 617 GTGCAC G[AT]GC[AT]^C
1016 GTGCTC
BsmAI 820 GAGAC GTCTC(1/5)
1060 GAGAC
BsrBI 57 GAGCGG CCGCTC(-3/-3)
1512 GAGCGG
BsrDI 936 CATTGC GCAATG(2/0)
1623 CATTGC
BssSI 54 CACGAG CACGAG(-5/-1)
1008 CACGAG
BstYI 656 AGATCT [GA]^GATC[CT]
704 AGATCC
DrdI 822 GACCATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1591 GACCCCCAGGTC
DsaI 137 CCGTGG C^C[GA][CT]GG
984 CCGCGG
EaeI 186 CGGCCG [CT]^GGCC[GA]
1260 TGGCCG
HgaI 1099 GACGC GACGC(5/10)
1661 GACGC
MaeI 1034 CTAG C^TAG
1204 CTAG
MaeIII 13 GTGAC ^GT[ACGT]AC
350 GTCAC
MflI 656 AGATCT [GA]^GATC[CT]
704 AGATCC
MslI 165 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1153 CACATCCGTG
PpuMI 408 AGGTCCT [GA]G^G[AT]CC[CT]
1589 AGGACCC
PvuII 806 CAGCTG CAG^CTG
1642 CAGCTG
RsaI 33 GTAC GT^AC
1548 GTAC
SexAI 812 ACCTGGT A^CC[AT]GGT
1276 ACCAGGT
Tsp45I 13 GTGAC ^GT[GC]AC
350 GTCAC
XhoII 656 AGATCT [GA]^GATC[CT]
704 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI Asp700I AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XmnI