Restriction map for
pFN21AE1312
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AviII BanI BbeI BbrPI
BbvCI BciVI BclI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 1222 GCGG CCGC(-3/-1)
AflII 637 CTTAAG C^TTAAG
Alw44I 1176 GTGCAC G^TGCAC
AlwNI 1285 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1176 GTGCAC G^TGCAC
AseI 333 ATTAAT AT^TAAT
AsnI 333 ATTAAT AT^TAAT
Asp700I 384 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 898 CCCGAG C^[CT]CG[GA]G
AvrII 750 CCTAGG C^CTAGG
BalI 879 TGGCCA TGG^CCA
BamHI 585 GGATCC G^GATCC
BanII 988 GGGCTC G[GA]GC[CT]^C
BbsI 492 GAAGAC GAAGAC(2/6)
BcgI 1168 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 75 CCGGG CC^[GC]GG
BfrI 637 CTTAAG C^TTAAG
BglI 941 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 750 CCTAGG C^CTAGG
BpuAI 492 GAAGAC GAAGAC(2/6)
BsaI 238 GAGACC GGTCTC(1/5)
BseRI 944 GAGGAG GAGGAG(10/8)
BsoBI 898 CCCGAG C^[CT]CG[GA]G
BspDI 769 ATCGAT AT^CGAT
BspHI 344 TCATGA T^CATGA
BsrDI 782 GCAATG GCAATG(2/0)
BstXI 870 CCAGATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 653 GCGC GCG^C
ClaI 769 ATCGAT AT^CGAT
DraII 113 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 879 TGGCCA [CT]^GGCC[GA]
EcoO109I 113 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1150 GAATTC G^AATTC
EcoT22I 856 ATGCAT ATGCA^T
HaeII 652 GGCGCT [GA]GCGC^[CT]
HapII 75 CCGG C^CGG
HhaI 653 GCGC GCG^C
HinP1I 653 GCGC G^CGC
HindIII 695 AAGCTT A^AGCTT
HpaII 75 CCGG C^CGG
MaeII 207 ACGT A^CGT
MaeIII 542 GTTAC ^GT[ACGT]AC
MluNI 879 TGGCCA TGG^CCA
MscI 879 TGGCCA TGG^CCA
MspA1I 1096 CAGCTG C[AC]G^C[GT]G
MspI 75 CCGG C^CGG
NciI 75 CCGGG CC^[GC]GG
NsiI 856 ATGCAT ATGCA^T
PpuMI 113 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 333 ATTAAT AT^TAAT
PvuII 1096 CAGCTG CAG^CTG
RcaI 344 TCATGA T^CATGA
SapI 738 GAAGAGC GCTCTTC(1/4)
SspI 1385 AATATT AAT^ATT
XcmI 954 CCAGAGTGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 384 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 838 AAATTC [GA]^AATT[CT]
1150 GAATTC
AflIII 1226 ACATGT A^C[GA][CT]GT
1346 ACATGT
ApoI 838 AAATTC [GA]^AATT[CT]
1150 GAATTC
AspHI 901 GAGCAC G[AT]GC[AT]^C
1176 GTGCAC
AvaII 114 GGACC G^G[AT]CC
396 GGACC
BfaI 751 CTAG C^TAG
1042 CTAG
BsiHKAI 901 GAGCAC G[AT]GC[AT]^C
1176 GTGCAC
BspLU11I 1226 ACATGT A^CATGT
1346 ACATGT
BsrI 444 ACTGG ACTGG(1/-1)
667 CCAGT
BstYI 585 GGATCC [GA]^GATC[CT]
1320 GGATCT
EcoNI 342 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1113 CCTCTTGGAGG
EcoT14I 750 CCTAGG C^C[AT][AT]GG
1376 CCAAGG
MaeI 751 CTAG C^TAG
1042 CTAG
MflI 585 GGATCC [GA]^GATC[CT]
1320 GGATCT
MseI 334 TTAA T^TAA
638 TTAA
NspI 1226 ACATGT [GA]CATG^[CT]
1346 ACATGT
PleI 978 GACTC GAGTC(4/5)
576 GAGTC
PstI 1270 CTGCAG CTGCA^G
1399 CTGCAG
SmlI 580 CTCAAG C^T[CT][GA]AG
637 CTTAAG
StyI 750 CCTAGG C^C[AT][AT]GG
1376 CCAAGG
TaqI 770 TCGA T^CGA
801 TCGA
Tru9I 334 TTAA T^TAA
638 TTAA
TspRI 668 CAGTG CA[GC]TG[ACGT][ACGT]^
1273 CAGTG
TthHB8I 770 TCGA T^CGA
801 TCGA
XhoII 585 GGATCC [GA]^GATC[CT]
1320 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI AlwI BbvI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI TseI
Tsp509I