Restriction map for
pFN21AE4551
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BbrPI BbvCI BcgI BclI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HinfI HpaI KspI MaeII MamI
MluI MroI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1156 GGTACC G^GTACC
AcyI 1017 GGCGCC G[GA]^CG[CT]C
AflII 1389 CTTAAG C^TTAAG
Asp718I 1156 GGTACC G^GTACC
AspHI 1110 GAGCAC G[AT]GC[AT]^C
BalI 972 TGGCCA TGG^CCA
BamHI 577 GGATCC G^GATCC
BbeI 1017 GGCGCC GGCGC^C
BbsI 459 GAAGAC GAAGAC(2/6)
BfrI 1389 CTTAAG C^TTAAG
BglII 1580 AGATCT A^GATCT
BpuAI 459 GAAGAC GAAGAC(2/6)
BsaHI 1017 GGCGCC G[GA]^CG[CT]C
BsaWI 1510 TCCGGT [AT]^CCGG[AT]
BseRI 1963 CTCCTC GAGGAG(10/8)
BsiHKAI 1110 GAGCAC G[AT]GC[AT]^C
BspDI 1433 ATCGAT AT^CGAT
BsrDI 529 GCAATG GCAATG(2/0)
BstXI 1721 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1433 ATCGAT AT^CGAT
DsaI 548 CCATGG C^C[GA][CT]GG
EaeI 972 TGGCCA [CT]^GGCC[GA]
EcoT22I 1523 ATGCAT ATGCA^T
HaeII 1017 GGCGCC [GA]GCGC^[CT]
HgaI 1914 GCGTC GACGC(5/10)
Hin1I 1017 GGCGCC G[GA]^CG[CT]C
HindIII 441 AAGCTT A^AGCTT
KasI 1017 GGCGCC G^GCGCC
KpnI 1156 GGTACC GGTAC^C
MfeI 1217 CAATTG C^AATTG
MluNI 972 TGGCCA TGG^CCA
MscI 972 TGGCCA TGG^CCA
MseI 1390 TTAA T^TAA
MunI 1217 CAATTG C^AATTG
NarI 1017 GGCGCC GG^CGCC
NcoI 548 CCATGG C^CATGG
NsiI 1523 ATGCAT ATGCA^T
SfoI 1017 GGCGCC GGC^GCC
StuI 1773 AGGCCT AGG^CCT
TaqI 1434 TCGA T^CGA
Tru9I 1390 TTAA T^TAA
Tsp509I 1218 AATT ^AATT
TthHB8I 1434 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 492 GGGCCC GGGCC^C
1362 GGGCCC
AspI 1462 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1536 GACTGTGTC
AvaI 812 CTCGGG C^[CT]CG[GA]G
1683 CTCGGG
BciVI 144 GTATCC GTATCC(6/5)
363 GTATCC
BcnI 573 CCGGG CC^[GC]GG
581 CCCGG
BglI 291 GCCAGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
413 GCCCATCTGGC
BlpI 507 GCTGAGC GC^T[ACGT]AGC
611 GCTCAGC
Bpu1102I 507 GCTGAGC GC^T[ACGT]AGC
611 GCTCAGC
BsaI 1273 GAGACC GGTCTC(1/5)
428 GGTCTC
BsoBI 812 CTCGGG C^[CT]CG[GA]G
1683 CTCGGG
BstAPI 1497 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1925 GCATCTTCTGC
CelII 507 GCTGAGC GC^T[ACGT]AGC
611 GCTCAGC
CfoI 1018 GCGC GCG^C
1851 GCGC
EarI 435 GAAGAG CTCTTC(1/4)
1401 GAAGAG
EcoNI 128 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1776 CCTTGGGCAGG
HhaI 1018 GCGC GCG^C
1851 GCGC
HinP1I 1018 GCGC G^CGC
1851 GCGC
HincII 313 GTCAAC GT[CT]^[GA]AC
1176 GTCAAC
HindII 313 GTCAAC GT[CT]^[GA]AC
1176 GTCAAC
Ksp632I 435 GAAGAG CTCTTC(1/4)
1401 GAAGAG
NciI 573 CCGGG CC^[GC]GG
581 CCCGG
PflFI 1462 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1536 GACTGTGTC
PflMI 122 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
496 CCAGTGTCTGG
SbfI 873 CCTGCAGG CCTGCA^GG
1494 CCTGCAGG
SexAI 632 ACCAGGT A^CC[AT]GGT
1008 ACCTGGT
Sse8387I 873 CCTGCAGG CCTGCA^GG
1494 CCTGCAGG
Tth111I 1462 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1536 GACTGTGTC
Van91I 122 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
496 CCAGTGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AvaII BanI BanII BbvI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TseI Tsp45I TspRI XcmI
XhoII