Restriction map for
pFN21AE5608
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SbfI ScaI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 1368 GAATTC [GA]^AATT[CT]
AcyI 329 GGCGCC G[GA]^CG[CT]C
AocI 187 CCTGAGG CC^T[ACGT]AGG
ApoI 1368 GAATTC [GA]^AATT[CT]
BanII 972 GGGCTC G[GA]GC[CT]^C
BbeI 329 GGCGCC GGCGC^C
BbsI 955 GTCTTC GAAGAC(2/6)
BglII 143 AGATCT A^GATCT
BpuAI 955 GTCTTC GAAGAC(2/6)
BsaHI 329 GGCGCC G[GA]^CG[CT]C
BsaI 1051 GGTCTC GGTCTC(1/5)
BsiEI 783 CGATCG CG[GA][CT]^CG
BsmBI 829 GAGACG CGTCTC(1/5)
BsmI 742 GCATTC GAATGC(1/-1)
Bsp1407I 701 TGTACA T^GTACA
BspLU11I 448 ACATGT A^CATGT
BsrGI 701 TGTACA T^GTACA
BsrI 864 ACTGG ACTGG(1/-1)
BstAPI 862 GCACTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 649 GGTGACC G^GT[ACGT]ACC
BstPI 649 GGTGACC G^GT[ACGT]ACC
Bsu36I 187 CCTGAGG CC^T[ACGT]AGG
DraIII 1009 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 835 CTCTTC CTCTTC(1/4)
Eco81I 187 CCTGAGG CC^T[ACGT]AGG
EcoNI 333 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 649 GGTGACC G^GT[ACGT]ACC
EcoRI 1368 GAATTC G^AATTC
EcoRV 1134 GATATC GAT^ATC
HaeII 329 GGCGCC [GA]GCGC^[CT]
Hin1I 329 GGCGCC G[GA]^CG[CT]C
KasI 329 GGCGCC G^GCGCC
Ksp632I 835 CTCTTC CTCTTC(1/4)
MseI 512 TTAA T^TAA
MslI 718 CACCGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 329 GGCGCC GG^CGCC
PaeR7I 554 CTCGAG C^TCGAG
PflMI 1411 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1397 GGGTCCC [GA]G^G[AT]CC[CT]
PvuI 783 CGATCG CGAT^CG
SapI 834 GCTCTTC GCTCTTC(1/4)
SexAI 317 ACCAGGT A^CC[AT]GGT
SfiI 39 GGCCTCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 329 GGCGCC GGC^GCC
SspBI 701 TGTACA T^GTACA
StuI 1113 AGGCCT AGG^CCT
Tru9I 512 TTAA T^TAA
Tsp509I 1369 AATT ^AATT
Van91I 1411 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 554 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 448 ACATGT A^C[GA][CT]GT
921 ACGTGT
AlwI 390 GATCC GGATC(4/5)
933 GATCC
AspHI 1006 GAGCAC G[AT]GC[AT]^C
1327 GAGCAC
AvaI 554 CTCGAG C^[CT]CG[GA]G
937 CCCGAG
AvaII 315 GGACC G^G[AT]CC
1398 GGTCC
BbvCI 404 CCTCAGC CCTCAGC(-5/-2)
292 GCTGAGG
BfaI 172 CTAG C^TAG
259 CTAG
BglI 40 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
965 GCCACCAGGGC
BsaAI 823 TACGTG [CT]AC^GT[GA]
920 TACGTG
BsgI 221 CTGCAC GTGCAG(16/14)
860 CTGCAC
BsiHKAI 1006 GAGCAC G[AT]GC[AT]^C
1327 GAGCAC
BsmAI 829 GAGAC GTCTC(1/5)
1052 GTCTC
BsoBI 554 CTCGAG C^[CT]CG[GA]G
937 CCCGAG
BsrFI 36 GCCGGC [GA]^CCGG[CT]
67 GCCGGC
Cfr10I 36 GCCGGC [GA]^CCGG[CT]
67 GCCGGC
DraII 395 AGGCCCC [GA]G^G[ACGT]CC[CT]
1397 GGGTCCC
EcoO109I 395 AGGCCCC [GA]G^G[ACGT]CC[CT]
1397 GGGTCCC
EcoT14I 116 CCAAGG C^C[AT][AT]GG
178 CCAAGG
HgaI 831 GACGC GACGC(5/10)
850 GACGC
HphI 649 GGTGA GGTGA(8/7)
461 TCACC
MaeI 172 CTAG C^TAG
259 CTAG
NaeI 36 GCCGGC GCC^GGC
67 GCCGGC
NgoMIV 36 GCCGGC G^CCGGC
67 GCCGGC
NspI 448 ACATGT [GA]CATG^[CT]
543 GCATGT
PvuII 407 CAGCTG CAG^CTG
1412 CAGCTG
StyI 116 CCAAGG C^C[AT][AT]GG
178 CCAAGG
TaqI 555 TCGA T^CGA
980 TCGA
TfiI 549 GATTC G^A[AT]TC
1352 GAATC
TthHB8I 555 TCGA T^CGA
980 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
BalI BanI BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TseI Tsp45I TspRI XhoII