Restriction map for
pFN21AE4514
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccII AccIII AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 681 GTCTAC GT^[AC][GT]AC
AclI 1079 AACGTT AA^CGTT
AcsI 424 GAATTT [GA]^AATT[CT]
AcyI 588 GGCGTC G[GA]^CG[CT]C
AlwNI 450 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 424 GAATTT [GA]^AATT[CT]
AspI 517 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 35 CTCGGG C^[CT]CG[GA]G
BamHI 156 GGATCC G^GATCC
BanI 476 GGCACC G^G[CT][GA]CC
BbsI 321 GAAGAC GAAGAC(2/6)
BfaI 511 CTAG C^TAG
BglI 1010 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1005 CTGGAG CTGGAG(16/14)
BpuAI 321 GAAGAC GAAGAC(2/6)
BsaHI 588 GGCGTC G[GA]^CG[CT]C
BsaI 829 GAGACC GGTCTC(1/5)
BsmBI 590 CGTCTC CGTCTC(1/5)
BsmI 647 GCATTC GAATGC(1/-1)
BsoBI 35 CTCGGG C^[CT]CG[GA]G
BspHI 1536 TCATGA T^CATGA
BsrFI 569 ACCGGC [GA]^CCGG[CT]
BstAPI 567 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 867 GGTGACC G^GT[ACGT]ACC
BstPI 867 GGTGACC G^GT[ACGT]ACC
CfoI 987 GCGC GCG^C
Cfr10I 569 ACCGGC [GA]^CCGG[CT]
EcoNI 1588 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 867 GGTGACC G^GT[ACGT]ACC
EcoT22I 237 ATGCAT ATGCA^T
HaeII 986 GGCGCT [GA]GCGC^[CT]
HgaI 589 GCGTC GACGC(5/10)
HhaI 987 GCGC GCG^C
Hin1I 588 GGCGTC G[GA]^CG[CT]C
HinP1I 987 GCGC G^CGC
MaeI 511 CTAG C^TAG
NsiI 237 ATGCAT ATGCA^T
PflFI 517 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 831 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1079 AACGTT AA^CGTT
PstI 574 CTGCAG CTGCA^G
RcaI 1536 TCATGA T^CATGA
SacI 1318 GAGCTC GAGCT^C
SfcI 574 CTGCAG C^T[GA][CT]AG
Tth111I 517 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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AfaI 1261 GTAC GT^AC
1452 GTAC
BglII 411 AGATCT A^GATCT
1137 AGATCT
BspMI 875 ACCTGC ACCTGC(4/8)
1541 ACCTGC
HinfI 517 GACTC G^A[ACGT]TC
935 GAGTC
PflMI 1202 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1486 CCAGTGCTTGG
PleI 517 GACTC GAGTC(4/5)
935 GAGTC
PvuII 450 CAGCTG CAG^CTG
923 CAGCTG
RsaI 1261 GTAC GT^AC
1452 GTAC
Van91I 1202 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1486 CCAGTGCTTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI ApaI AspHI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII