Restriction map for
pFN21AE5201
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HapII HhaI
HinP1I HpaI HpaII KasI KpnI
KspI MaeI MaeIII MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AciI 9 CCGC CCGC(-3/-1)
AcyI 19 GGCGTC G[GA]^CG[CT]C
AhdI 135 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 272 GATCC GGATC(4/5)
AspEI 135 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 199 GTGCTC G[AT]GC[AT]^C
AvaI 149 CTCGAG C^[CT]CG[GA]G
AvaII 500 GGACC G^G[AT]CC
BbvCI 85 GCTGAGG CCTCAGC(-5/-2)
BbvI 11 GCAGC GCAGC(8/12)
BmyI 199 GTGCTC G[AGT]GC[ACT]^C
BsaBI 601 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 19 GGCGTC G[GA]^CG[CT]C
BsiHKAI 199 GTGCTC G[AT]GC[AT]^C
BsiYI 443 CCTTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 443 CCTTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 335 GGGAC GGGAC(10/14)
BsoBI 149 CTCGAG C^[CT]CG[GA]G
Bsp1286I 199 GTGCTC G[AGT]GC[ACT]^C
BspMI 230 ACCTGC ACCTGC(4/8)
BstAPI 429 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 271 AGATCC [GA]^GATC[CT]
Cfr13I 500 GGACC G^G[ACGT]CC
DdeI 86 CTGAG C^T[ACGT]AG
DraII 499 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 506 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 135 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 306 GAAGAG CTCTTC(1/4)
EcoNI 443 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 499 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 443 CCTTGG C^C[AT][AT]GG
FauI 8 CCCGC CCCGC(4/6)
Fnu4HI 11 GCAGC GC^[ACGT]GC
HaeIII 175 GGCC GG^CC
HgaI 20 GCGTC GACGC(5/10)
Hin1I 19 GGCGTC G[GA]^CG[CT]C
HincII 327 GTCAAC GT[CT]^[GA]AC
HindII 327 GTCAAC GT[CT]^[GA]AC
HindIII 483 AAGCTT A^AGCTT
HinfI 606 GAATC G^A[ACGT]TC
HphI 492 GGTGA GGTGA(8/7)
ItaI 11 GCAGC GC^[ACGT]GC
Ksp632I 306 GAAGAG CTCTTC(1/4)
MaeII 141 ACGT A^CGT
MamI 601 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 271 AGATCC [GA]^GATC[CT]
MseI 373 TTAA T^TAA
NlaIV 500 GGACCC GG[ACGT]^[ACGT]CC
PaeR7I 149 CTCGAG C^TCGAG
PpuMI 499 AGGACCC [GA]G^G[AT]CC[CT]
SapI 306 GAAGAGC GCTCTTC(1/4)
Sau96I 500 GGACC G^G[ACGT]CC
SexAI 42 ACCAGGT A^CC[AT]GGT
SfcI 287 CTGTAG C^T[GA][CT]AG
StyI 443 CCTTGG C^C[AT][AT]GG
TaqI 150 TCGA T^CGA
TfiI 606 GAATC G^A[AT]TC
Tru9I 373 TTAA T^TAA
TseI 11 GCAGC G^C[AT]GC
TthHB8I 150 TCGA T^CGA
XhoI 149 CTCGAG C^TCGAG
XhoII 271 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 217 AAATTC [GA]^AATT[CT]
545 AAATTC
AluI 310 AGCT AG^CT
484 AGCT
ApoI 217 AAATTC [GA]^AATT[CT]
545 AAATTC
BsaJI 15 CCCTGG C^C[ACGT][ACGT]GG
443 CCTTGG
BssKI 16 CCTGG ^CC[ACGT]GG
43 CCAGG
BstNI 16 CCTGG CC^[AT]GG
43 CCAGG
Cac8I 481 GCAAGC GC[ACGT]^[ACGT]GC
485 GCTTGC
DpnII 272 GATC ^GATC
361 GATC
EcoRII 16 CCTGG ^CC[AT]GG
43 CCAGG
MboI 272 GATC ^GATC
361 GATC
MboII 306 GAAGA GAAGA(8/7)
400 GAAGA
MslI 276 CATTCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
610 CATGTTTGTG
MvaI 16 CCTGG CC^[AT]GG
43 CCAGG
MwoI 11 GCAGCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
429 GCAGACTTTGC
NdeII 272 GATC ^GATC
361 GATC
NlaIII 115 CATG CATG^
610 CATG
Sau3AI 272 GATC ^GATC
361 GATC
ScrFI 16 CCTGG CC^[ACGT]GG
43 CCAGG
SmlI 149 CTCGAG C^T[CT][GA]AG
202 CTCAAG
TspRI 251 CACTG CA[GC]TG[ACGT][ACGT]^
414 CACTG
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
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BsrI MnlI Tsp509I