Restriction map for
pFN21AE6443
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MluNI MscI
MseI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 906 TCCGGA T^CCGGA
AfaI 435 GTAC GT^AC
AhdI 1068 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1281 GATCC GGATC(4/5)
AlwNI 932 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 384 CCTGAGG CC^T[ACGT]AGG
AspEI 1068 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 463 GAGCAC G[AT]GC[AT]^C
BfaI 358 CTAG C^TAG
BglII 771 AGATCT A^GATCT
BsaWI 906 TCCGGA [AT]^CCGG[AT]
BseAI 906 TCCGGA T^CCGGA
BsgI 256 CTGCAC GTGCAG(16/14)
BsiHKAI 463 GAGCAC G[AT]GC[AT]^C
BsmAI 766 GTCTC GTCTC(1/5)
BsmBI 765 CGTCTC CGTCTC(1/5)
BspEI 906 TCCGGA T^CCGGA
BsrFI 1262 ACCGGC [GA]^CCGG[CT]
BstAPI 1157 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 378 GGTGACC G^GT[ACGT]ACC
BstPI 378 GGTGACC G^GT[ACGT]ACC
Bsu36I 384 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1262 ACCGGC [GA]^CCGG[CT]
DrdI 1023 GACAGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 312 CGGCCA [CT]^GGCC[GA]
Eam1105I 1068 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1213 GAAGAG CTCTTC(1/4)
Eco81I 384 CCTGAGG CC^T[ACGT]AGG
EcoO65I 378 GGTGACC G^GT[ACGT]ACC
EcoT14I 1034 CCATGG C^C[AT][AT]GG
Ksp632I 1213 GAAGAG CTCTTC(1/4)
KspI 820 CCGCGG CCGC^GG
MaeI 358 CTAG C^TAG
MroI 906 TCCGGA T^CCGGA
NcoI 1034 CCATGG C^CATGG
PflMI 1120 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 337 GGGACCT [GA]G^G[AT]CC[CT]
PstI 8 CTGCAG CTGCA^G
PvuII 552 CAGCTG CAG^CTG
RsaI 435 GTAC GT^AC
SacII 820 CCGCGG CCGC^GG
SfcI 8 CTGCAG C^T[GA][CT]AG
StyI 1034 CCATGG C^C[AT][AT]GG
Tsp509I 470 AATT ^AATT
Van91I 1120 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1001 CCAGCAGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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ApaI 31 GGGCCC GGGCC^C
847 GGGCCC
BanI 976 GGCACC G^G[CT][GA]CC
1129 GGTGCC
BbsI 241 GTCTTC GAAGAC(2/6)
289 GTCTTC
BglI 516 GCCCAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
968 GCCGGACAGGC
BpuAI 241 GTCTTC GAAGAC(2/6)
289 GTCTTC
BsaAI 139 TACGTG [CT]AC^GT[GA]
1147 TACGTG
BspMI 949 ACCTGC ACCTGC(4/8)
1109 ACCTGC
BstYI 771 AGATCT [GA]^GATC[CT]
1280 AGATCC
DpnII 772 GATC ^GATC
1281 GATC
DsaI 820 CCGCGG C^C[GA][CT]GG
1034 CCATGG
MaeII 140 ACGT A^CGT
1148 ACGT
MboI 772 GATC ^GATC
1281 GATC
MflI 771 AGATCT [GA]^GATC[CT]
1280 AGATCC
NdeII 772 GATC ^GATC
1281 GATC
NlaIII 1035 CATG CATG^
1173 CATG
Sau3AI 772 GATC ^GATC
1281 GATC
SexAI 374 ACCAGGT A^CC[AT]GGT
584 ACCAGGT
SmaI 543 CCCGGG CCC^GGG
720 CCCGGG
TaqI 245 TCGA T^CGA
605 TCGA
TfiI 130 GAATC G^A[AT]TC
922 GAATC
TthHB8I 245 TCGA T^CGA
605 TCGA
XhoII 771 AGATCT [GA]^GATC[CT]
1280 AGATCC
XmaCI 543 CCCGGG C^CCGGG
720 CCCGGG
XmaI 543 CCCGGG C^CCGGG
720 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AspI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV PflFI PleI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI
Tth111I