Restriction map for
pFN21AE3670
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 384 ACATGT A^C[GA][CT]GT
AlwNI 486 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1461 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1309 GGACC G^G[AT]CC
BalI 1250 TGGCCA TGG^CCA
BanI 211 GGTGCC G^G[CT][GA]CC
BanII 728 GAGCTC G[GA]GC[CT]^C
BcgI 736 CGAGTACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 115 TACGTG [CT]AC^GT[GA]
BsmAI 541 GTCTC GTCTC(1/5)
BsmI 1233 GAATGC GAATGC(1/-1)
BspHI 1190 TCATGA T^CATGA
BspLU11I 384 ACATGT A^CATGT
BssSI 1168 CACGAG CACGAG(-5/-1)
CfoI 633 GCGC GCG^C
Cfr13I 1309 GGACC G^G[ACGT]CC
DraIII 831 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 701 CCATGG C^C[GA][CT]GG
EaeI 1250 TGGCCA [CT]^GGCC[GA]
EcoT14I 701 CCATGG C^C[AT][AT]GG
HaeII 632 GGCGCT [GA]GCGC^[CT]
HhaI 633 GCGC GCG^C
HinP1I 633 GCGC G^CGC
HindIII 1131 AAGCTT A^AGCTT
HphI 408 TCACC GGTGA(8/7)
MaeII 116 ACGT A^CGT
MaeIII 1025 GTTAC ^GT[ACGT]AC
MluNI 1250 TGGCCA TGG^CCA
MscI 1250 TGGCCA TGG^CCA
MseI 287 TTAA T^TAA
NcoI 701 CCATGG C^CATGG
NdeI 1286 CATATG CA^TATG
PaeR7I 359 CTCGAG C^TCGAG
RcaI 1190 TCATGA T^CATGA
SacI 728 GAGCTC GAGCT^C
Sau96I 1309 GGACC G^G[ACGT]CC
ScaI 1091 AGTACT AGT^ACT
SfcI 1228 CTACAG C^T[GA][CT]AG
StyI 701 CCATGG C^C[AT][AT]GG
Tru9I 287 TTAA T^TAA
XcmI 675 CCAGCTGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 359 CTCGAG C^TCGAG
XmnI 1461 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 818 TCCGGA T^CCGGA
1306 TCCGGA
AlwI 163 GATCC GGATC(4/5)
1211 GGATC
AvaI 166 CCCGAG C^[CT]CG[GA]G
359 CTCGAG
BlpI 236 GCTTAGC GC^T[ACGT]AGC
1342 GCTCAGC
BpmI 673 CTCCAG CTGGAG(16/14)
1348 CTGGAG
Bpu1102I 236 GCTTAGC GC^T[ACGT]AGC
1342 GCTCAGC
BsaWI 818 TCCGGA [AT]^CCGG[AT]
1306 TCCGGA
BseAI 818 TCCGGA T^CCGGA
1306 TCCGGA
BseRI 169 GAGGAG GAGGAG(10/8)
1446 GAGGAG
BsoBI 166 CCCGAG C^[CT]CG[GA]G
359 CTCGAG
BspEI 818 TCCGGA T^CCGGA
1306 TCCGGA
BstXI 128 CCAGCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1241 CCAAGCTCTTGG
CelII 236 GCTTAGC GC^T[ACGT]AGC
1342 GCTCAGC
EcoT22I 1289 ATGCAT ATGCA^T
1426 ATGCAT
HaeIII 462 GGCC GG^CC
1251 GGCC
HapII 819 CCGG C^CGG
1307 CCGG
HpaII 819 CCGG C^CGG
1307 CCGG
MroI 818 TCCGGA T^CCGGA
1306 TCCGGA
MslI 409 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
997 CATGGCAGTG
MspI 819 CCGG C^CGG
1307 CCGG
NsiI 1289 ATGCAT ATGCA^T
1426 ATGCAT
NspI 384 ACATGT [GA]CATG^[CT]
935 ACATGC
SfaNI 154 GATGC GCATC(5/9)
1425 GATGC
SmlI 359 CTCGAG C^T[CT][GA]AG
1084 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AspHI BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI FokI HinfI
ItaI Ksp632I MaeI MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI ScrFI TaqI TfiI TseI
Tsp509I TspRI TthHB8I